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In vitro effect of children’s toothpaste on brushing abrasion of eroded primary enamel

  • Original Scientific Article
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Abstract

Purpose

Emerging data have suggested that acid erosion has become an increasing clinical problem in pediatric dentistry. This study aimed to investigate the abrasive effects of two commercial toothpastes on primary enamel eroded by orange juice using an in vitro model.

Methods

Thirty enamel slabs were obtained from primary teeth and then randomly assigned to three groups (n = 8) comprising two different toothpastes: G1-control-distilled water; G2-Dentifrice A (containing no fluoride); and G3-Dentifrice B (1.100 ppmF–NaF). Each slab had one half protected to provide a control side, and the other one was subjected to treatments. The slabs were submitted to daily erosive challenges (3×/day, 2 min) in concentrated orange juice (pH 3.38) associated with abrasive challenges using a tooth-brushing machine (150 brush movements for each cycle). During the experimental period, the slabs were kept in 37 °C artificial saliva, and the experiment was carried out for 5 days. The depths of the resulting eroded areas were measured by stylus profilometry. The data were analyzed using ANOVA and the Tukey–Kramer test (α = 5%).

Results

Tooth loss (µm, mean ± SD) was 2.46 ± 1.18 for G1, 3.32 ± 2.12 for G2 and 2.14 ± 1.03 for G3. Therefore, the NaF dentifrice (Dentifrice B) produced significantly less mineral loss (p = 0.04) than dentifrice A.

Conclusions

The findings suggest that fluoride toothpaste could protect primary enamel against erosion.

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References

  • Addy M, Shellis RP. Interaction between attrition, abrasion and erosion in tooth wear. Monogr Oral Sci. 2006;20:17–311.

    PubMed  Google Scholar 

  • Al-Batayneh OB, Jbarat RA, Al-Khateeb SN. Effect of application sequence of fluoride and CCP-ACP on remineralization of white spots lesions in primary teeth: An in-vitro study. Arch Oral Biol. 2017;83:236–40.

    PubMed  Google Scholar 

  • Assunção CM, Schlueter N, Rodrigues JA, Carvalho TS, Lussi A. Do fluoride toothpastes have similar preventive effect in permanente and primary teeth against erosive tooth wear? Int J Paediatr Dent. 2018. https://doi.org/10.1111/ipd.12449.

    Article  PubMed  Google Scholar 

  • Assunção CM, Lussi A, Rodrigues JA, Carvalho TS. Efficacy of toothpastes in the prevention of erosive tooth wear in permanent and deciduous teeth. Clin Oral Invest. 2019;23(1):273–84.

    Google Scholar 

  • Barbour ME, Rees GD. The role of erosion, erosion, abrasion and attrition in tooth wear. J Clin Dent. 2006;17:88–93.

    PubMed  Google Scholar 

  • Bardsley PF, Taylor S, Milosevik A. Epidemiological studies of tooth wear and dental erosion in 14-year-old children in North West England. Part 1: The relationship with water fluoridation and social deprivation. Br Dent J. 2004;197(7):413–6.

    PubMed  Google Scholar 

  • Barlett DW, Smith BG, Wilson RF. Comparison of the effect of fluoride and non-fluoride toothpaste on tooth wear in vitro and the influence of enamel fluoride concentration and hardness of enamel. Br Dent J. 1994;176(9):346–8.

    Google Scholar 

  • Carvalho TS, Bonecker M, Altenburger MJ, Buzalaf MA, Sampaio FC, Lussi A. Fluoride varnishes containing calcium glycerophosphate: fluoride uptake and the effect on in vitro enamel erosion. Clin Oral Investig. 2015a;19:1429–36.

    PubMed  Google Scholar 

  • Carvalho TS, Peters BG, Rios D, Magalhães AC, Sampaio FC, Buzalaf MA, Bonecker MJ. Fluoride varnishes with calcium glycerophosphate: fluoride release and effect on in vitro enamel demineralization. Braz Oral Res. 2015b;29:1–6.

    Google Scholar 

  • Cowie H, Sood S, Richardson G. Preventive oral health advice for children. Dental Nurs. 2010;6(1):p20–24.

    Google Scholar 

  • El Aidi H, Bronkhorst EM, Truin GJ. A longitudinal study of tooth erosion in adolescents. J Dent Res. 2008;87(8):731–5.

    PubMed  Google Scholar 

  • Ganss C, Lussi A, Grunau O, Klimek J, Schlueter N. Conventional and anti-erosion fluoride toothpastes: effect on enamel erosion and erosion-abrasion. Caries Res. 2011;45:581–9.

    PubMed  Google Scholar 

  • Ganss C, Marten J, Hara AT, Schlueter N. Toothpastes and enamel erosion/abrasion. Impact of active ingredients and the particulate fraction. J Dent. 2016;54:62–7.

    PubMed  Google Scholar 

  • Godoi FA, Carlos NR, Bridi EC, Amaral FLB, França FMG, Turssi CP, Kantovitz KR, Basting RT. Remineralizing effect of comercial fluoride varnishes on artificial enamel lesions. Braz Oral Res. 2019;33(44):1–10.

    Google Scholar 

  • Hughes J, West N, Addy M. The protective effect of fluoride treatments against enamel erosion in vitro. J Oral Rehabil. 2004;31:357–63.

    PubMed  Google Scholar 

  • Jaeggi T, Lussi A. Prevalence, incidence and distribution of erosion. Monogr Oral Sci. 2014;25:55–73.

    PubMed  Google Scholar 

  • Jones L, Lekkas D, Hunt D, McIntyre J, Rafir W. Studies on dental erosion: an in vivo-in vitro model of endogenous dental erosion—its application to testing protection by fluoride gel application. Aust Dent J. 2002;47:304–8.

    PubMed  Google Scholar 

  • Kato MT, Lancia M, Sales-Peres SHC, Buzalaf MAR. Preventive effect of commercial desensitizing toothpastes on bovine enamel erosion in vitro. Caries Res. 2010;44:85–9.

    PubMed  Google Scholar 

  • Lagerweij MD, Buchalla W, Kohnke S, Becker K, Lennon ÁM, Attin T. Prevention of erosion and abrasion by a high fluoride concentration gel applied at high frequencies. Caries Res. 2006;40:148–53.

    PubMed  Google Scholar 

  • Luo Y, Zeng XJ, Du MQ, Bedi R. The prevalence of dental erosion in preschool children in China. J Dent. 2005;33:115–21.

    PubMed  Google Scholar 

  • Lussi A, Carvalho TS. Erosive tooth weat: a multifactorial condition of growing concern an increasing knowledge. Monogr Oral Sci. 2014;25:1–15.

    PubMed  Google Scholar 

  • Lussi A, Jaeggi T, Zero D. The role of diet in the etiology of dental erosion. Caries Res. 2004;38:34–44.

    PubMed  Google Scholar 

  • Lussi A, Buzalaf MAR, Duangthip D, Anttonen V, Ganss C, João-Sousa SH, Baumann T, Carvalho TS. The use of fluoride for the prevention of dental erosion and erosive tooth wear in children and adolescents. Eur Arch Pediatr Dent. 2019. https://doi.org/10.1007/s40368-019-00420-0.

    Article  Google Scholar 

  • Mantonanaki M, Koletsi-Kounari H, Mamai-Homata E, Papaioan W. Dental erosion prevalence and associated risk indicators among preschool children in Athens, Greece. Clin Oral Investig. 2013;17(2):585–93.

    PubMed  Google Scholar 

  • Meurman JH, ten Cate JM. Pathogenesis and modifying factors of dental erosion. Eur J Oral Sci. 1996;104:199–206.

    PubMed  Google Scholar 

  • Milosevic A. Abrasion: a common dental problem revisited. Prim Dent J. 2017;28–6(1):32–6.

    Google Scholar 

  • Moimaz SA, Araujo PC, Chiba FY, Garbin CA, Saliba NA. Prevalence of deciduous tooth erosion in childhood. Int J Dent Hyg. 2013;11(3):226–30. https://doi.org/10.1111/idh.12020.

    Article  PubMed  Google Scholar 

  • Mok T, McIntyre J, Hunt D. Dental erosion: in vitro model of wine assessor’s erosion. Aust Dent J. 2001;46:263–8.

    PubMed  Google Scholar 

  • Moretto MJ, Magalhães AC, Sassaki KT, Delbem ACB, Martinhon CCR. Effect of different fluoride concentrations of experimental dentifrices on enamel erosion and abrasion. Caries res. 2010;44:135–40.

    PubMed  Google Scholar 

  • Murakami C, Bönecker M, Corrêa MSNP, Mendes FM, Rodrigues CRMD. Effect of fluoride varnish and gel on dental erosion in primary and permanent teeth. Arch Oral Biol. 2009;54:997–1001.

    PubMed  Google Scholar 

  • Murakami C, Tello G, Abanto J, Oliveira LB, Bonini GC, Bönecker M. Trends in the prevalence of erosive tooth wear in Brazilian preschool children. Int J Paediatr Dent. 2016;26:60–5.

    PubMed  Google Scholar 

  • Nayak SS, Ashokkumar BR, Ankola AV, Hebbal MI. Distribution and severity of erosion among 5-year-old children in a city in India. J Dent Child Chic Ill. 2010;77:152–7.

    Google Scholar 

  • Philip S, Abdulla M, Ganapathy S, Vaishnavi V, Rajeev V. Comparative evaluation of erosive potential of various frozen and unfrozen fruit juices on primary teeth enamel: an in vitro study. J Pharm BioAllied Sci. 2019;11:463–7.

    Google Scholar 

  • Prett IA. High fluoride concentration toothpastes for children and adolescents. Caries Res. 2016;50(1):9–14.

    Google Scholar 

  • Shroff P, Gondivkar SM, Kumbhare SP, Sarode S, Gadbail AR, Patil S. Analyses of the erosive potential of various soft drinks and packaged fruit juiceson teeth. J Contemp Dent Pract. 2018;19(12):1547–52.

    Google Scholar 

  • Tao D-Y, Hao G, Lu H-X, Tian Y, Feng X-P. Dental erosion among children aged 3–6 years and its associated indicators. J Public Health Dent. 2015;75:291–7.

    PubMed  Google Scholar 

  • Toumba KJ, Twetman S, Splieth C, Parnell C, van Loveren C, Lygidakis NA. Guidelines on the use fluorides for caries prevention in children: an Updated EAPD policy document. Eur Arch Paediatr Dent. 2019;20:507–16.

    PubMed  Google Scholar 

  • Tschammler C, Müller-Pflanz C, Attin T, Müller J, Wiegand A. Prevalence and risk factors of erosive tooth wear in 3–6 year old German kindergarten children. A comparison between 2004/05 and 2014/15. J Dent. 2016;52:45–9.

    PubMed  Google Scholar 

  • Walsh T, Worthington HV, Glenny AM, Appelbe P, Marinho VC, Shi X. Fluoride Toothpastes of different concentrations for preventing dental caries in children and adolescents. Cochrane Database Syst Rev. 2010;20(1):CD007868.

    Google Scholar 

  • West NX, Hughes JA, Addy M. Erosion of dentin enamel in vitro by dietary acids; the effect of temperature, acid character, concentration and exposure time. J Oral Rehabil. 2000;27:875–80.

    PubMed  Google Scholar 

  • West NX, He T, Hellin N, Claydon N, Seong J, Macdonald E, Farrell S, Eusebio R, Wildeberg A. Randomized in situ clinical trial evaluating erosion protection efficacy of a 0.454% stannous fluoride dentifrice. Int J Dent Hyg. 2019;17(3):261–7.

    PubMed  PubMed Central  Google Scholar 

  • Wiegand A, Müller J, Werner C, Attin T. Prevalence of erosive tooth wear and associated risk factors in 2-7-year-old German kindergarten children. Oral Dis. 2006;12:117–24.

    PubMed  Google Scholar 

  • Wong MC, Glenny AM, Tsang BW, Lo EC, Worthington HV, Marinho VC. Topical Fluoride as a cause of dental fluorosis in children. Cochrane Database Syst Rev. 2010;20(1):CD007693.

    Google Scholar 

  • Yu H, Wegehaupt FJ, Zaruba M, Becker K, Roos M, Attin T, Wiegand A. Erosion-inhibiting potential of a stannous chloride-containing fluoride solution under acid flow conditions in vitro. Arch Soforal Biol. 2010;55:702–5.

    Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Operative Dentistry, Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Cap. Francisco Pedro S/N-Rodolfo Teófilo, Fortaleza, CE, 60430-170, Brazil

    V. F. Passos, R. B. R. A. Sousa, M. A. S. de Melo & S. L. Santiago

  2. Departament of Academic Master’s Degree in Dental Sciences, Christus University Center–Unichristus, Fortaleza, CE, Brazil

    E. A. B. Gomes & J. P. M. Lima

  3. Odontology-Christus University Center–Unichristus, St. João Adolfo Gurgel, 133–Cocó, Fortaleza, CE, 60190-060, Brazil

    E. A. B. Gomes & J. P. M. Lima

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  1. V. F. Passos
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  2. R. B. R. A. Sousa
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  3. M. A. S. de Melo
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Correspondence to J. P. M. Lima.

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Passos, V.F., Sousa, R.B.R.A., de Melo, M.A.S. et al. In vitro effect of children’s toothpaste on brushing abrasion of eroded primary enamel. Eur Arch Paediatr Dent 22, 157–162 (2021). https://doi.org/10.1007/s40368-020-00536-8

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  • DOI: https://doi.org/10.1007/s40368-020-00536-8

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