Abstract
A screening of commercially available lipases for the synthesis of vitamin E succinate showed that lipase from Candida rugosa presented the highest yield. The synthesis of vitamin E succinate in organic solvents with different lgP values ranging from −1.3 to 3.5 was investigated. Of particular interest was that dimethyl sulfoxide (DMSO) with the lowest lgP exhibited the highest yield among all the organic solvents used. It suggests that lgP is incapable of satisfactorily predicting the biocompatibility of organic solvents due to the complexity of enzymatic reaction with hydrophilic and hydrophobic substrates in organic solvent. Effects of different operating conditions, such as molar ratio of substrate, enzyme concentration, reaction temperature, mass transfer, and reaction time were also studied. Under the optimum conditions of 10 g/L enzyme, a stirring rate of 100 r/min, a substrate molar ratio of 5:1 at 55 °C for 18 h, a satisfactory yield(46.95%) was obtained. The developed method has a potential to be used for efficient enzymatic production of vitamin E succinate.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Valentin H. E., Qi Q., Appl. Microbiol. Biotechnol., 2005, 68, 436
Barros L., Correia D. M., Ferreira I. C. F. R., Baptistaa P., Santos-Buelga C., Food Chem., 2008, 110, 1046
Israel K., Yu W., Sanders B. G., Kline K., Nutrition Cancer, 2000, 36, 90
Quin J., Engle D., Litwiller A., Peralta E., Grasch A., Boley T., Hazelrigg S., J. Surgical Res., 2005, 127, 139
Prasad K. N., Kumar B., Yan X. D., Hanson A. J., Cole W. C., J. Am. Coll. Nutrition, 2003, 22, 108
Bonrath W., Cirillo F., Process for the Preparation of Tocol Acylates and Tocopherol Acylates, US6444098B2, 2002
Torres P., Reyes D. D., Lopez C. N., Ferrer M., Ballesteros A., Plou F. J., Process Biochem., 2008, 43, 145
Xin J., Chen L., Zhang Y., Wen R. R., Zhao D. M., Xia C. G., Food Biotechnol., 2011, 25, 43
Yin C. H., Zhang C., Gao M., Chinese. J. Chem. Eng., 2011, 19, 135
Castillo E., Pezzotti F., Navarro A., López-Munguía A., J. Biotechnol., 2003, 102, 251
Kaewprapan K., Tuchinda P., Marie E., Durandc A., Inprakhon P., J. Mol. Catal. B: Enzymatic, 2007, 47, 135
Ferreira L., Gil M. H., Cabrita A., Dordick J. S., Biomaterials, 2005, 26, 4707
Ferreira L., Gil M. H., Dordick J. S., Biomaterials, 2002, 23, 3957
Laane C., Boeren S., Vos K., Veeger C., Biotechnol. Bioeng., 1987, 30, 81
Klibanov A. M., Nature, 2001, 409, 241
Carrea G., Riva S., Angew. Chem. Int. Ed., 2000, 39, 2226
Klibanov A. M., Acc. Chem. Res., 1990, 23, 114
Rubio E., Fernandez M. A., Klibanov A. M., J. Am. Chem. Soc., 1991, 113, 695
Gogoi P., Hazarika S., Dutta N. N., Rao P. G., Chem. Eng. J., 2009, 155, 810
Hazarika S., Goswami P., Dutta N. N., Chem. Eng. J., 2003, 94, 1
Lu J. K., Nie K. L., Wang F., Tan T. W., Bioresour. Technol., 2008, 99, 6070
Yang T., Rebsdorf M., Engelrud U., Xu X., J. Food Lipids, 2005, 12, 299
Duan Z. Q., Du W., Liu D. H., Process Biochem., 2010, 45, 1923
Wolff A., Zhu L., Wong Y., Straathof A., Jongejan J., Heijnen J., Biotechnol. Bioeng., 1999, 62, 125
Han S. Y., Pan Z. Y., Huang D. F., Ueda M., Wang X. N., J. Mol. Catal. B: Enzymatic, 2009, 59, 16
Author information
Authors and Affiliations
Corresponding author
Additional information
Supported by the National High-Tech Research and Development Program of China(No.2011AA02A209), the Key Project of National Natural Science Foundation of China(No.20936002) and the National Natural Science Foundation of China for Young Scholars(No.20906049).
Rights and permissions
About this article
Cite this article
Jiang, Xj., Hu, Y., Jiang, L. et al. Synthesis of vitamin E succinate from Candida rugosa lipase in organic medium. Chem. Res. Chin. Univ. 29, 223–226 (2013). https://doi.org/10.1007/s40242-013-2486-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s40242-013-2486-z