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Role of metabolizing MTHFR gene polymorphism (rs1801133) and its mRNA expression among Type 2 Diabetes

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Abstract

Objective

Type 2 Diabetes is a glucose metabolic disorder occurred by insulin insensitivity in which folate metabolism plays an important role. it is believed that polymorphism of Methylenetetrahydrofolate reductase (MTHFR) C677T linked with type 2 diabetes mellitus. However, results are conflicted. therefore, in this study we re-examine the relationship between MTHFR C677T in type 2 diabetes mellitus patients.

Methods

Present research work included 100 newly diagnosed type 2 diabetic mellitus (T2DM) cases and 100 healthy individuals. After the blood sample collection all the biochemical parameters were evaluated among the T2DM cases and healthy individuals. DNA and RNA extraction from whole blood was done to study the MTHFR gene polymorphism by allele specific polymerase chain reaction method and its expression analysis was done by quantitative real time polymerase chain reaction method.

Results

The significant difference was observed in genotype distribution among case and control group (p=0.0002). Compared with wildtype CC genotype, CT heterozygous (OR=2.95, 95% Cl=1.62-5.38) and TT homozygous (OR=3.20, CI=1.79-5.73) suggest to have effect of MTHFR polymorphism on type 2 mellitus risk. Moreover, relative MTHFR mRNA expression was found for wild type CC genotype 3.02-fold, CT heterozygous genotype 2.57 fold and mutant TT homozygous genotype 0.50-fold which is down regulated (p<0.0001).

Conclusion

Our results indicates that the polymorphism in MTHFR C677T plays significant role in type II diabetes risk. MTHFR CT heterozygous and mutant TT genotype showed reduced mRNA expression among the T2DM patients. However, large scale case–control studies are needed to strengthen such conclusion in the future.

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Acknowledgments

Author would like to thanks the entire study participant for research work.

Author information

Author notes

  1. Divya Pathak, Dharmsheel Shrivastav and Amit K. Verma contributed equally.

Authors and Affiliations

  1. Central Drugs Standard Control Organisation, New Delhi, India

    Divya Pathak

  2. Amity Institute of Biotechnology, Amity University, Rajasthan, Jaipur, India

    Dharmsheel Shrivastav & Desh Deepak Singh

  3. Department of Zoology and Environmental Sciences, GKV, Haridwar, India

    Amit K. Verma

  4. Department of Clinical Nutrition, College of Applied Medical Sciences, Jazan University, Jazan, Saudi Arabia

    Abdulrahman A. Alsayegh

  5. Department of Biochemistry, All India Institute of Medical Sciences, Bhopal, Madhya Pradesh, India

    Prasant Yadav

  6. Faculty of Medicine, Alatoo International University, Bishkek, Kyrgyzstan

    Nawaid Hussain Khan & Mirza Masroor Ali Beg

  7. Department of Medical Laboratory, College of Applied Medical Sciences, Taibah University, Yanbu, Saudi Arabia

    Alhanouf I. Al-Harbi

  8. Department of Clinical Nutrition, College of Applied Health Sciences in Arras, Qassim University, Buraydah, Saudi Arabia

    Mohammad Idreesh Khan

  9. Department of Accident and Emergency, Lok Nayak Hospital, New Delhi, India

    Kapil Bihade

  10. Centre for promotion of Medical Research, Alatoo International University, Bishkek, Kyrgyzstan

    Mirza Masroor Ali Beg

Authors
  1. Divya Pathak
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  2. Dharmsheel Shrivastav
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  6. Nawaid Hussain Khan
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  11. Mirza Masroor Ali Beg
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Corresponding author

Correspondence to Mirza Masroor Ali Beg.

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This study was approved by the Institutional Ethical Committee of Gurukula Kangri University, Haridwar, India.

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Pathak, D., Shrivastav, D., Verma, A.K. et al. Role of metabolizing MTHFR gene polymorphism (rs1801133) and its mRNA expression among Type 2 Diabetes. J Diabetes Metab Disord 21, 511–516 (2022). https://doi.org/10.1007/s40200-022-01001-7

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  • DOI: https://doi.org/10.1007/s40200-022-01001-7

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