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Protective effects of Labisia pumila against neuropathy in a diabetic rat model

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Abstract

Purpose

Diabetes accelerates peripheral, distal symmetric polyneuropathy, small fiber predominant neuropathy, radiculoplexopathy, and autonomic neuropathy. This study investigated the neuroprotective effects of gallic acid and myricetin-rich Labisia pumila extract in a diabetic neuropathy rat model and evaluated the neuropathy correlationship with serum inflammatory biomarkers.

Methods

Thirty male rats were divided into 5 groups (n = 6), namely: healthy control; non-treated diabetic control; and diabetic-rats treated with 200 mg/kg metformin; Labisia pumila ethanol extract (LP) at 150 mg/kg or 300 mg/kg doses. Diabetes was induced by 60 mg streptozotocin /kg intraperitoneal injection. Rats were orally treated daily for ten weeks. Their fasting blood glucose (FBG), neurological functions (hot plate and tail immersion; thermal hyperalgesia; cold allodynia; motor walking function), biomarkers for inflammation and oxidative stress, the neuro-histopathological changes, and brain somatic index were measured.

Results

The extract significantly prevented abnormal increases in FBG and decreases in body weight gain. It attenuated behavioral dysfunctions (hot plate and tail immersion; thermal hyperalgesia; cold allodynia; motor walking function), systemic inflammation (serum TNF-α, prostaglandin-E2) oxidative tension (malondialdehyde), histological brain and sciatic nerve injuries in the diabetic-rats, better than Metformin.

Conclusion

LP mitigated neural dysfunction better than metformin partly by amending diabetic systemic inflammation, oxidative tension, and diabetic abnormalities. The nerve injuries were strongly correlated to serum prostaglandin-E2, TNF-α levels, and walking functions. The motor function was correlated to sensory neuronal functions, inflammation, and oxidation. The sensory neuronal functions were more affected by TNF-α than prostaglandin-E2 or oxidation. Diabetic brain and sciatic nerve deteriorations were influenced by serum TNF-α, PGE2, and MDA levels.

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Data availability

Available on request.

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Funding

From Universiti Putra Research grant [grant number 9578200].

Author information

Authors and Affiliations

  1. UPM-MAKNA Cancer Research Laboratory, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

    Nazmun Nahar & Suhaila Mohamed

  2. Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, Serdang, Malaysia

    Noordin Mohamed Mustapha & Lau Seng Fong

Authors
  1. Nazmun Nahar
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  2. Suhaila Mohamed
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  3. Noordin Mohamed Mustapha
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  4. Lau Seng Fong
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Contributions

Nazmun Nahar (planning, execution, data collection, and manuscript preparation), Seng Fong Lau and Noordin Mohamed Mustapha (attending veterinary clinician for animal studies), and Suhaila Mohamed (grant recipient, main supervisor, project planning coordinator, and manuscript writing/editing, principal researcher). All researchers approved the final manuscript.

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Correspondence to Suhaila Mohamed.

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Nazmun Nahar, Seng Fong Lau, Noordin M. Mustapha, and Suhaila Mohamed have no conflict of interest to declare.

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Institutional Animal Care and Use Committee registration number: (UPM/IACUC/AUP-R095/2017).

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Nahar, N., Mohamed, S., Mustapha, N.M. et al. Protective effects of Labisia pumila against neuropathy in a diabetic rat model. J Diabetes Metab Disord 21, 1–11 (2022). https://doi.org/10.1007/s40200-021-00905-0

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  • DOI: https://doi.org/10.1007/s40200-021-00905-0

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