Abstract
Background
One significant ethnomedicinal motivation behind Pleurotus tuberregium (PTT) and Anchomanes difformis (ADT) tubers is cardiovascular-related conditions treatment. This investigation is in this way planned for deciding the impact of PTT and ADT enhanced eating routine on key enzymes linked with hypercholesterolemia in elevated cholesterol fed rodents.
Methods
Rats were isolated into control group, hypercholesterolemic-prompted untreated group, hypercholesterolemic-treated groups with dietary routine containing PTT (5% and 10%), ADT (5% and 10%), 5% PTT and 5% ADT conbination and traditional medication, atorvastatin for 28 days. Ten rodents were utilized for every one of the groups.
Results
Feeding with PTT and ADT comprehensive eating regimen and their combination significantly (P < 0.05) diminished the AChE, HMG-CoA, ALT, AST, ALP, LDH and CK activities and levels of mevalonate, triglyceride (TG), total cholesterol (TC), low density lipoprotein (LDLch), atherogenic indices, MDA and ROS yet significantly increased the SOD, CAT, GPx activities, and level of HDL, GSH when contrasted with HC-initiated untreated rodents. Likewise, histopathological of liver and heart demonstrated no obsessive changes in all the treated groups when contrasted with healthy control group. HPLC fingerprinting of the PTT and ADT aqueous concentrates uncovered the nearness of ferulic acid, vanillic acid, catechin, quercetin, chlorogenic acid, ellagic acid and gallic acid. Notwithstanding, aqueous concentrate of ADT contained plentiful convergences of the polyphenolics when contrasted with PTT concentrate.
Conclusions
The tubers HMG-CoA reductase inhibitory activity could additionally support their antihypercholesterolemic use in folk medication. Accordingly, the tubers may in this way be valuable as restorative nourishment for helpful treatment of clinical conditions related hypercholesterolemia with the ADT diet holding more guarantee.
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Data availability
The data analyzed and materials utilized in this investigation are available from the comparing author on reasonable solicitation.
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Acknowledgements
We gratefully acknowledge Dr. Nnaemeka T. Asogwa, Central Research and Diagnostic Laboratory, Number 132, University Road, Tanke, Ilorin, Kwara State, Nigeria for his technical assistance in carrying out histopathological examination. This work was acted in partial fulfillment of the prerequisites for M.Sc of Ogundumi oluwatosin Adesola in Department of Biochemistry, Faculty of Science, Ekiti State University, Ado Ekiti, Nigeria.
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ASF and OOA carried out the analyses, analyzed and deciphered the data, and drafted the manuscript. OFL and ASF planned the investigation and participated in analysis and interpretation of data. OFL coordinated the investigation, overhauled the manuscript and approved the final form to be submitted for publication and aided in the analysis and interpretation of data. All authors read and approved the final manuscript.
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Oyetayo, F.L., Akomolafe, S.F. & Ogundumi, O.A. Anti-hypercholesterolemic potential of diet supplemented with Anchomanes difformis and Pleurotus tuberregium tubers in high cholesterol fed rats. J Diabetes Metab Disord 19, 1139–1155 (2020). https://doi.org/10.1007/s40200-020-00615-z
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DOI: https://doi.org/10.1007/s40200-020-00615-z