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Main gut bacterial composition differs between patients with type 1 and type 2 diabetes and non-diabetic adults

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Abstract

Background

Regarding the role of gut microbial dysbiosis in hyperglycemia, we aimed to compare the main gut bacterial composition among type 1 and type 2 diabetic patients and healthy non-diabetic adults.

Methods

A total of 110 adult subjects (49 patients diagnosed with type 2 diabetes, 21 patients diagnosed with type 1 diabetes and 40 healthy persons) were included in this case-control study. The intestinal microbiota composition was investigated by quantitative real-time polymerase chain reaction (qPCR) method targeting bacterial 16S rRNA gene. Comparison between three groups was done using one-way analysis of variance.

Results

The participants’ mean age in the type 1 diabetes, type 2 diabetes and control groups was 35.4, 57.2 and 38.0 years, respectively. Higher level of Escherichia, Prevotella and Lactobacillus was observed in both type 1 and type 2 diabetic patients compared with the healthy group (P ˂0.001). In contrast, bacterial load of Bifidobacterium, Roseburia and Bacteroides was higher in healthy control group (P < 0.05). Faecalibacterium was significantly lower in type 1 diabetic patients compared with the other two groups (P ˂0.001). No significant difference was found in Akkermansia level among three groups.

Conclusions

Gut microbial alterations have been observed among patients suffering from type 1 and type 2 diabetes mellitus and healthy control adults. Butyrate producing genera including Roseburia and Faecalibacterium decreased while Escherichia, Prevotella and Lactobacillus increased in diabetic patients compared to healthy subjects. Modulating approaches of gut microbiota composition could be helpful in diabetes management.

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Acknowledgements

We are so thankful of all the participants.

Funding

This work was supported by the National Institute for Medical Research Development (NIMAD) (grant number: 940604).

Author information

Author notes

  1. Hanieh-Sadat Ejtahed and Zahra Hoseini-Tavassol contributed equally to this work.

Authors and Affiliations

  1. Obesity and Eating Habits Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

    Hanieh-Sadat Ejtahed, Shirin Hasani-Ranjbar & Ahmad-Reza Soroush

  2. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

    Hanieh-Sadat Ejtahed, Seyed Davar Siadat & Bagher Larijani

  3. Department of Mycobacteriology and Pulmonary Research, Microbiology Research Center, Pasteur Institute of Iran, Tehran, Iran

    Zahra Hoseini-Tavassol, Ava Behrouzi, Sara Ahmadi Badi, Farzam Vaziri, Abolfazl Fateh & Seyed Davar Siadat

  4. Department of Biochemistry, Pasteur Institute of Iran, Tehran, Iran

    Shohre Khatami

  5. Department of Infectious Diseases, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

    Mehrangiz Zangeneh

  6. Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Arfa Moshiri

  7. Laboratory of Experimental Therapies in Oncology, IRCCS Istituto Giannina Gaslini, Genoa, Italy

    Arfa Moshiri

  8. Chemical Injuries Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran

    Mostafa Ghanei

  9. Molecular Biology Department, Microbiology Research Center, Pasteur Institute of Iran, Tehran, Iran

    Saeid Bouzari

  10. Bacteriology Department, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

    Shahin Najar-Peerayeh

Authors
  1. Hanieh-Sadat Ejtahed
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  2. Zahra Hoseini-Tavassol
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  9. Ahmad-Reza Soroush
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  15. Seyed Davar Siadat
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Correspondence to Seyed Davar Siadat.

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Ejtahed, HS., Hoseini-Tavassol, Z., Khatami, S. et al. Main gut bacterial composition differs between patients with type 1 and type 2 diabetes and non-diabetic adults. J Diabetes Metab Disord 19, 265–271 (2020). https://doi.org/10.1007/s40200-020-00502-7

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  • DOI: https://doi.org/10.1007/s40200-020-00502-7

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