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Proliferation and In Vitro Wound Healing Effects of the Microniosomes Containing Narcissus tazetta L. Bulb Extract on Primary Human Fibroblasts (HDFs)

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Abstract

Purpose

In Traditional Persian Medicine (TPM), different natural treatments have been suggested for skin damages such as Narcissus tazetta L. bulb application. New drug delivery systems such as niosomes have shown considerable increase transdermal drug delivery through stratum corneum, the main barrier against substances transport into skin. The aim of this study is preparation of niosomal formulations from N. tazetta bulb extract and evaluation of its in vitro wound healing effect.

Materials and methods

Non-ionic surfactant vesicles (NSVs or niosomes) were prepared by film hydration method from percolated extract of N. tazetta bulb. A number of 12 niosomal formulations (F1-F12) were prepared using different proportions of Span 60/Tween 60/cholesterol and 80% methanol-dissolved/aqueous PEN (percolation extract of N. tazetta) (30 and 50 mg/ml). Their morphology, particle size, physical and chemical stability and encapsulation efficiency was studied. In vitro wound healing effect of various concentrations of the best PEN niosomal formulation (F9) was evaluated in comparison to PEN on human dermal fibroblasts (HDFs).

Results

Increasing the aqueous/methanolic PEN concentration from 3 to 5% resulted size reduction of NSVs with statistically significant difference (p < 0.05). F9 showed the most physicochemical stability and was chosen for in vitro wound healing effect. This formulation exhibited significantly effects (p < 0.05) on cell proliferation in HDF cells at 1.562 and 3.125 μg/ml compared with the untreated cells using neutral red assay.

Conclusion

Formulation of PEN in niosome carrier significantly decreased the gap width on human dermal fibroblasts.

Schematic processes of proliferation effect of narcisus tazetta bulb on fibroblast cells.

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References

  1. Hess Ct. Clinical guide to skin and wound care. Philadelphia: Lippincott Williams & Wilkins; 2012.

  2. Pardakhty A, Moazeni E, Varshosaz J, Hajhashemi V, Rouholamini Najafabadi A. Pharmacokinetic study of niosome-loaded insulin in diabetic rats. Daru J Pharm Sci. 2011;19(6):404–11.

    CAS  Google Scholar 

  3. Pardakhty A. Non-ionic surfactant vesicles (Niosomes) as new drug delivery systems. In: Pharmaceutical sciences: breakthrough in research and practice. United States of American: IGI Global; 2016. p. 154–83.

    Google Scholar 

  4. Makeshwar KB, Wasankar SR. Niosome: a novel drug delivery system. Asian J Pharm Res. 2013;3(1):16–20.

    Google Scholar 

  5. Hamishehkar H, Rahimpour Y, Kouhsoltani M. Niosomes as a propitious carrier for topical drug delivery. Expert Opin Drug Deliv. 2013;10(2):261–72.

    Article  CAS  PubMed  Google Scholar 

  6. Zhang Y, Zhang K, Wu Z, Guo T, Ye B, Lu M, et al. Evaluation of transdermal salidroside delivery using niosomes via in vitro cellular uptake. Int J Pharm. 2015;478(1):138–46.

    Article  CAS  PubMed  Google Scholar 

  7. Aqil F, Munagala R, Jeyabalan J, Vadhanam MV. Bioavailability of phytochemicals and its enhancement by drug delivery systems. Cancer Lett. 2013;334(1):133–41.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Manca ML, Castangia I, Marco Z, Nacher A, Valenti D, Fernandez-Busquets X, et al. Development of curcumin loaded sodium hyaluronate immobilized vesicles (hyalurosomes) and their potential on skin inflammation and wound restoring. Biomaterials. 2015;71:100–9.

    Article  CAS  PubMed  Google Scholar 

  9. Motaal AAA, Ali M, El-Gazayerly ON. An in vivo study of Hypericum perforatum in topical drug delivery systems. Planta Med. 2016;82(S01):934.

  10. Azam Khan M. Exir azam (persian). Tehran: Institute of medical history, research institute for Islamic and complementary Medicine; 2008.

  11. Razi MEZ. Al-Havi fil Tibb. Beirut: Dar Ehia Al-Turath al-‘Arabi; 2001.

  12. Jorjani SI. Zakhire Kharazmshahi. Qom: Institute for natural medicine recovery; 2012.

  13. Tonekaboni Mazandarani MM. Tohfat al momenin. Qom: Institute for Natural Medicine recovery; 2011.

  14. Avicenna (Ibn Sina). the persian translation of Al-Qanun fi al-Tibb (The canon of medicine). Translated from Arabic to Persian by Abdurrahman Sharaf-kandi. Tehran, Iran: Soroush publication; 1983.

  15. Mazhari N. Flora of Iran. Iran: research institute of forests and rangelands; 2004.

  16. Hanks GR. Narcissus and Daffodil: the genus narcissus. United States: CRC Press; 2003.

  17. Wang X, Dubois R, Adams D Jr. Alzheimer's disease prevention and use of traditional plant medicines. Lett Drug Des Discov. 2015;12(2):140–51.

    Article  CAS  Google Scholar 

  18. McNulty J, Nair JJ, Bastida J, Pandey S, Griffin C. Structure-activity studies on the lycorine pharmacophore: a potent inducer of apoptosis in human leukemia cells. Phytochemistry. 2009;70(7):913–9.

    Article  CAS  PubMed  Google Scholar 

  19. Lubbe A, Gude H, Verpoorte R, Choi YH. Seasonal accumulation of major alkaloids in organs of pharmaceutical crop Narcissus Carlton. Phytochemistry. 2013;88:43–53.

    Article  CAS  PubMed  Google Scholar 

  20. Ooi LS, Ng TB, Geng Y, Ooi VE. Lectins from bulbs of the Chinese daffodil Narcissus tazetta (family Amaryllidaceae). Biochem Cell Biol. 2000;78(4):463–8.

    Article  CAS  PubMed  Google Scholar 

  21. Aghili Shirazi M. Makhzan-al-advia Tehran: Tehran university of medical sciences; 2009.

  22. Gürdal B, Kültür Ş. An ethnobotanical study of medicinal plants in Marmaris (Muğla, Turkey). J Ethnopharmacol. 2013;146(1):113–26.

    Article  PubMed  Google Scholar 

  23. Talib WH, Mahasneh AM. Antimicrobial, cytotoxicity and phytochemical screening of Jordanian plants used in traditional medicine. Molecules. 2010;15(3):1811–24.

    Article  CAS  PubMed  Google Scholar 

  24. Rameshk M, Sharififar F, Mehrabani M, Pardakhty A, Farsinejad A. In vitro proliferation and wound healing effects of Narcissus tazetta L. bulb on primary human dermal fibroblasts. J Pharm Res Int. 2018;20(6):1–11.

    Article  Google Scholar 

  25. pharmacopoeia EcoIh. Iranian herbal pharmacopoeia. Tehran: Ministry of health and medical education/food and drug administration; 2002.

  26. Ansari M, Sharififar F, Kazemipour M, Sarhadinejad Z, Mahdavi H. Teucrium polium L. extract adsorbed on zinc oxide nanoparticles as a fortified sunscreen. Int J Pharm Investig. 2013;3(4):188–93.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Pardakhty A, Shakibaie M, Daneshvar H, Khamesipour A, Mohammadi-Khorsand T, Forootanfar H. Preparation and evaluation of niosomes containing autoclaved Leishmania major: a preliminary study. J Microencapsul. 2012;29(3):219–24.

    Article  CAS  PubMed  Google Scholar 

  28. Borenfreund E, Babich H, Martin-Alguacil N. Comparisons of two in vitro cytotoxicity assays—the neutral red (NR) and tetrazolium MTT tests. Toxicol in Vitro. 1988;2(1):1–6.

    Article  CAS  PubMed  Google Scholar 

  29. Dinda M, Mazumdar S, Das S, Ganguly D, Dasgupta UB, Dutta A, et al. The water fraction of Calendula officinalis hydroethanol extract stimulates in vitro and in vivo proliferation of dermal fibroblasts in wound healing. Phytother Res. 2016;30(10):1696–707.

    Article  CAS  PubMed  Google Scholar 

  30. Junyaprasert VB, Singhsa P, Suksiriworapong J, Chantasart D. Physicochemical properties and skin permeation of span 60/tween 60 niosomes of ellagic acid. Int J Pharm. 2012;423(2):303–11.

    Article  CAS  PubMed  Google Scholar 

  31. Varshosaz J, Taymouri S, Pardakhty A, Asadi-Shekaari M, Babaee A. Niosomes of ascorbic acid and α-tocopherol in the cerebral ischemia-reperfusion model in male rats. Bio Med Res Int. 2014; 2014:1–9.

    Google Scholar 

  32. Ritwiset A, Krongsuk S, Johns JR. Molecular structure and dynamical properties of niosome bilayers with and without cholesterol incorporation: a molecular dynamics simulation study. Appl Surf Sci. 2016;380:23–31.

    Article  CAS  Google Scholar 

  33. Manconi M, Sinico C, Valenti D, Loy G, Fadda AM. Niosomes as carriers for tretinoin. I. Preparation and properties. Int J Pharm. 2002;234(1–2):237–48.

    Article  CAS  PubMed  Google Scholar 

  34. Vangala A, Kirby D, Rosenkrands I, Agger EM, Andersen P, Perrie Y. A comparative study of cationic liposome and niosome-based adjuvant systems for protein subunit vaccines: characterisation, environmental scanning electron microscopy and immunisation studies in mice. J Pharm Pharmacol. 2006;58(6):787–99.

    Article  CAS  PubMed  Google Scholar 

  35. Shalini M, Ali MH, Lakshmi P. Formulation and evaluation of elastic niosomes of eletriptan hydrobromide. Int J Pharm Sci Res. 2016;7(4):1679.

    CAS  Google Scholar 

  36. Moazeni E, Gilani K, Sotoudegan F, Pardakhty A, Najafabadi AR, Ghalandari R, et al. Formulation and in vitro evaluation of ciprofloxacin containing niosomes for pulmonary delivery. J Microencapsul. 2010;27(7):618–27.

    Article  CAS  PubMed  Google Scholar 

  37. Shehata T, Kimura T, Higaki K, Ogawara K-i. In-vivo disposition characteristics of PEG niosome and its interaction with serum proteins. Int J Pharm. 2016;512(1):322–8.

    Article  CAS  PubMed  Google Scholar 

  38. Lin T, Fang Q, Peng D, Huang X, Zhu T, Luo Q, et al. PEGylated non-ionic surfactant vesicles as drug delivery systems for Gambogenic acid. Drug Deliv. 2013;20(7):277–84.

    Article  CAS  PubMed  Google Scholar 

  39. Briuglia M-L, Rotella C, McFarlane A, Lamprou DA. Influence of cholesterol on liposome stability and on in vitro drug release. Drug Deliv Transl Res. 2015;5(3):231–42.

    Article  CAS  PubMed  Google Scholar 

  40. Nematollahi MH, Pardakhty A, Torkzadeh-Mahanai M, Mehrabani M, Asadikaram G. Changes in physical and chemical properties of niosome membrane induced by cholesterol: a promising approach for niosome bilayer intervention. RSC Adv. 2017;7(78):49463–72.

    Article  CAS  Google Scholar 

  41. Qumbar M, Imam SS, Ali J, Ahmad J, Ali A. Formulation and optimization of lacidipine loaded niosomal gel for transdermal delivery: in-vitro characterization and in-vivo activity. Biomed Pharmacother. 2017;93:255–66.

    Article  CAS  PubMed  Google Scholar 

  42. Basiri L, Rajabzadeh G, Bostan A. α-Tocopherol-loaded niosome prepared by heating method and its release behavior. Food Chem. 2017;221:620–8.

    Article  CAS  PubMed  Google Scholar 

  43. Samed N, Sharma V, Sundaramurthy A. Hydrogen bonded niosomes for encapsulation and release of hydrophilic and hydrophobic anti-diabetic drugs: an efficient system for oral anti-diabetic formulation. Appl Surf Sci. 2017;449:567–573.

    Article  CAS  Google Scholar 

  44. Kern D, Method to treat skin conditions with narcissus tazetta bulb extract. US patent App. 12/019163, 2008; 1–8.

  45. Wyganowska-Swiatkowska M, Urbaniak P, Szkaradkiewicz A, Jankun J, Kotwicka M. Effects of chlorhexidine, essential oils and herbal medicines (Salvia, chamomile, Calendula) on human fibroblast in vitro. Cent Eur J Immunol. 2016;41(2):125.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  46. Singh O, Khanam Z, Misra N, Srivastava MK. Chamomile (Matricaria chamomilla L.): an overview. Pharmacogn Rev. 2011;5(9):82–95.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  47. Bouchareb D, Bouraoui NK, Fernandez X, Michel T. Phytochemical characterization of Narcissus tazetta and evaluation of its bioactive potent. Planta Med. 2016;81(S 01):P59.

    Google Scholar 

  48. Arabski M, Węgierek-Ciuk A, Czerwonka G, Lankoff A, Kaca W. Effects of Saponins against clinical E. coli strains and eukaryotic cell line. J Biomed Biotechnol. 2012;2012:286216.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  49. Kang TH, Pae HO, Yoo JC, Kim NY, Kim YC, Ko GI, et al. Antiproliferative effects of alkaloids from Sedum sarmentosum on murine and human hepatoma cell lines. J Ethnopharmacol. 2000;70(2):177–82.

    Article  CAS  PubMed  Google Scholar 

  50. Un RN, Barlas FB, Yavuz M, Ag Seleci D, Seleci M, Gumus ZP, et al. Phyto-niosomes: in vitro assessment of the novel nanovesicles containing marigold extract. Int J Polym Mater. 2015;64(17):927–37.

    Article  CAS  Google Scholar 

  51. Ioele G, Tavano L, De Luca M, Ragno G, Picci N, Muzzalupo R. Photostability and ex-vivo permeation studies on diclofenac in topical niosomal formulations. Int J Pharm. 2015;494(1):490–7.

    Article  CAS  PubMed  Google Scholar 

  52. Abdelbary AA, AbouGhaly MHH. Design and optimization of topical methotrexate loaded niosomes for enhanced management of psoriasis: application of box–Behnken design, in-vitro evaluation and in-vivo skin deposition study. Int J Pharm. 2015;485(1):235–43.

    Article  CAS  PubMed  Google Scholar 

  53. Ali A, Sarhan HA, Magdy T. Preparation and characterization of phenytoin sodium niosomes for enhanced closure of skin injuries. Int J Pharm Pharm Sci. 2014;6:542–6.

    Google Scholar 

  54. Puglia C, Lauro MR, Tirendi GG, Fassari GE, Carbone C, Bonina F, et al. Modern drug delivery strategies applied to natural active compounds. Expert Opin Drug Deliv. 2017;14(6):755–68.

    Article  CAS  PubMed  Google Scholar 

  55. Alexander A, Patel RJ, Saraf S, Saraf S. Recent expansion of pharmaceutical nanotechnologies and targeting strategies in the field of phytopharmaceuticals for the delivery of herbal extracts and bioactives. J Control Release. 2016;241:110–24.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Herbal and Traditional Medicines Research Center, Department of Pharmacognosy, Faculty of Pharmacy Kerman University of Medical Sciences, Haft Bagh-e Alavi Blvd, Kerman, Iran

    Maryam Rameshk, Fariba Sharififar & Mitra Mehrabani

  2. Faculty of Persian Medicine, Department of Traditional Pharmacy, Kerman University of Medical Sciences, Modiriat Street, Kerman, Iran

    Maryam Rameshk

  3. Pharmaceutics Research Center, Neuropharmacology Institute, Kerman University of Medical Sciences, Haft Bagh-e Alavi Blvd, Kerman, Iran

    Abbas Pardakhty

  4. Department of Hematology and Blood Banking, faculty of allied Medical Sciences, Kerman University of Medical Sciences, Kerman, Iran

    Alireza Farsinejad

  5. Afzalipour School of Medicine, University of Shahid Bahonar, Kerman, Iran

    Alireza Farsinejad

  6. Physiology Research Center; Neuropharmacology Institute, Kerman University of Medical Sciences, Ibne cina street, Kerman, Iran

    Mehrnaz Mehrabani

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  1. Maryam Rameshk
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  2. Fariba Sharififar
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Correspondence to Abbas Pardakhty.

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Rameshk, M., Sharififar, F., Mehrabani, M. et al. Proliferation and In Vitro Wound Healing Effects of the Microniosomes Containing Narcissus tazetta L. Bulb Extract on Primary Human Fibroblasts (HDFs). DARU J Pharm Sci 26, 31–42 (2018). https://doi.org/10.1007/s40199-018-0211-7

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