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Effect of light on growth of green microalgae Scenedesmus quadricauda: influence of light intensity, light wavelength and photoperiods

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Abstract

Microalgae biomass is a potential source of biomass to be considered as a source of bioenergy, biochemicals and even food supply. The key to an economic and healthy algal cultivation is to optimize the growth conditions. The main objective of this research was to investigate the effects of light on the growth of green microalgae Scenedesmus quadricauda. This wild-type green microalgae Scenedesmus quadricauda obtained from the Porsuk River in the Eskisehir region of Turkey characterized by a high lipid content has been cultivated at different wavelengths, different light intensities and different photoperiod cycles. Our results show that for each light intensity, there is an optimal wavelength. The best performing light intensity was 500 μmol photons m−2 s−1 with a wavelength of 660 nm corresponding to specific growth rate of 0.621 d−1 and an average biomass productivity of 0.756 g/L following 10 days of growth. When the intensity of the light has been increased to 1000 μmol.m−2.s−1 photons, growth is inhibited by showing signs of photodamage. At an intensity of 100 μmol.m−2.s−1, the growth was either very slow for some wavelengths or no growth for majority of them. This suggests that there is a minimal intensity threshold for growth being specific for each wavelength. Variable growth performances under different light/dark cycles imply necessity of the factor optimization. In this case, a photoperiod of 1: 1 h light:dark mode, with a moderate intensity of 500 μmol of photons m−2 s−1, was the best for an efficient productivity.

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References

  1. Alqadi, M.A., et al.: Effect of photoperiod on the growth of Chlamydomonas Incerta and pollutant removal. Malays. J. Civil Eng., 29 (2017)

  2. Sadi, M.: Les micro algues: un défi prometteur pour des biocarburants propres. Revue Des Energies Renouvelables SIENR’12 Ghardaïa, 195–202 (2012)

  3. Wen, Z., Johnson M.B.: Microalgae as a feedstock for biofuel production. (2009)

  4. Lucchetti, A.: Modélisation et conception d’un système de culture de microalgues. ENMP, Paris (2014)

    Google Scholar 

  5. Deb, U.K., et al.: The effect of irradiance related temperature on microalgae growth in a tubular photo bioreactor for cleaner energy. Am. J. Comp. Math. 7(3), 371–384 (2017)

    Article  Google Scholar 

  6. Coelho, R.S., et al.: High cell density cultures of microalgae under fed-batch and continuous growth. Chem. Eng. Trans 38, 313–318 (2014)

    Google Scholar 

  7. Bernardi, A., et al.: An identifiable state model to describe light intensity influence on microalgae growth. Ind. Eng. Chem. Res. 53(16), 6738–6749 (2014)

    Article  Google Scholar 

  8. Straka, L., Rittmann, B.E.: Light-dependent kinetic model for microalgae experiencing photoacclimation, photodamage, and photodamage repair. Algal. Res. 31, 232–238 (2018)

    Article  Google Scholar 

  9. Tikkanen, M., Mekala, N.R., Aro, E.-M.: Photosystem II photoinhibition-repair cycle protects Photosystem I from irreversible damage. BBA-Bioenerg. 1837(1), 210–215 (2014)

    Article  Google Scholar 

  10. Neidhardt, J., et al.: Photosystem-II repair and chloroplast recovery from irradiance stress: relationship between chronic photoinhibition, light-harvesting chlorophyll antenna size and photosynthetic productivity in Dunaliella salina (green algae). Photosynth. Res. 56(2), 175–184 (1998)

    Article  Google Scholar 

  11. Sonoike, K.: Photoinhibition of photosystem I. Physiol. Plantarum 142(1), 56–64 (2011)

    Article  Google Scholar 

  12. Kim, T.-H., et al.: The effects of wavelength and wavelength mixing ratios on microalgae growth and nitrogen, phosphorus removal using Scenedesmus sp for wastewater treatment. Bioresour. Technol. 130, 75–80 (2013)

    Article  Google Scholar 

  13. Xu, Y., Ibrahim, I.M., Harvey, P.J.: The influence of photoperiod and light intensity on the growth and photosynthesis of Dunaliella salina (chlorophyta) CCAP 19/30. Plant Physiol. Bioch. 106, 305–315 (2016)

    Article  Google Scholar 

  14. Bose, A., Chakraborty, S.: Mathematical modelling of the effects of circadian rhythm on microalgal growth in phototrophic and mixotrophic cultures. Chem. Engineer. Trans. 52, 955–960 (2016)

    Google Scholar 

  15. Wu, H.: Effect of different light qualities on growth, pigment content, chlorophyll fluorescence, and antioxidant enzyme activity in the red alga Pyropia haitanensis (Bangiales, Rhodophyta). Biomed RES INT, 2016 (2016)

  16. Thanh, N.T., et al.: The effect of aeration rate on the growth of Scenedesmus quadricauda in column photobioreactor. J. Jpn. I. Ener., 94(2), 177–180 (2015)

  17. Ilavarasi, A., et al.: Optimization of various growth media to freshwater microalgae for biomass production. Biotechnology 10(6), 540–545 (2011)

    Article  Google Scholar 

  18. Derakhshandeh, M., Atici, T., Un, U.T.: Evaluation of wild-type microalgae species biomass as carbon dioxide sink and renewable energy resource. Waste Biomass Valori. 12(1), 105–121 (2020)

    Article  Google Scholar 

  19. Rippka, R., et al.: Generic assignments, strain histories and properties of pure cultures of cyanobacteria. Microbiology 111(1), 1–61 (1979)

    Article  Google Scholar 

  20. Vass, I., Aro, E.-M.: Photoinhibition of photosynthetic electron transport. Primary Process. Photosynth.: Basic Princ. Appar. 1, 393–425 (2008)

    Google Scholar 

  21. Kendirlioglu, G., Cetin, A.K.: Effect of different wavelengths of light on growth, pigment content and protein amount of Chlorella vulgaris. Fresenius Environ. Bull 26, 7974–7980 (2017)

    Google Scholar 

  22. Asuthkar, M., et al.: Effect of different wavelengths of light on the growth of Chlorella pyrenoidosa. Int. J. Pharm. Sci. Res. 7(2), 847–851 (2016)

    Google Scholar 

  23. Gollan, P.J., et al.: Interaction between photosynthetic electron transport and chloroplast sinks triggers protection and signalling important for plant productivity. Philos. T R Soc. B 372(1730), 20160390 (2017)

    Article  Google Scholar 

  24. de Mooij, T., et al.: Impact of light color on photobioreactor productivity. Algal. Res. 15, 32–42 (2016)

    Article  Google Scholar 

  25. Wang, C.-Y., Fu, C.-C., Liu, Y.-C.: Effects of using light-emitting diodes on the cultivation of Spirulina platensis. Biochem. Eng. J. 37(1), 21–25 (2007)

    Article  Google Scholar 

  26. Mulders, K.J., et al.: Phototrophic pigment production with microalgae: biological constraints and opportunities. J. Phycol. 50(2), 229–242 (2014)

    Article  Google Scholar 

  27. Wahidin, S., Idris, A., Shaleh, S.R.M.: The influence of light intensity and photoperiod on the growth and lipid content of microalgae Nannochloropsis sp. Bioresour. Technol. 129, 7–11 (2013)

    Article  Google Scholar 

  28. Sager, J., McFarlane, J.: Radiation, Plant growth chamber handbook. (1997)

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Funding

This project was funded by Anadolu University through scientific research project No.1702F050. anadolu üniversitesi,1702F050,Ümran TEZCAN UN

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Authors and Affiliations

  1. Department of Process Engineering, Technology Faculty, Chlef Hassiba Ben Bouali University, 02180, Ouled Farès District, Chlef, Algeria

    Nadjiya Fettah & Larbi Mahmoudi

  2. Life Science and Biomedical Engineering Application and Research Center, Istanbul Gelisim University, 34310, Istanbul, Turkey

    Masoud Derakhshandeh

  3. Department of Environmental Engineering, Engineering Faculty, Eskisehir Technical University, 26555, Eskisehir, Turkey

    Umran Tezcan Un

Authors
  1. Nadjiya Fettah
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  2. Masoud Derakhshandeh
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  3. Umran Tezcan Un
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  4. Larbi Mahmoudi
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Correspondence to Masoud Derakhshandeh.

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Fettah, N., Derakhshandeh, M., Tezcan Un, U. et al. Effect of light on growth of green microalgae Scenedesmus quadricauda: influence of light intensity, light wavelength and photoperiods. Int J Energy Environ Eng 13, 703–712 (2022). https://doi.org/10.1007/s40095-021-00456-3

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  • DOI: https://doi.org/10.1007/s40095-021-00456-3

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