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HPTLC Fingerprinting of Cultivated Picrorhiza kurrooa Accessions in Uttarakhand, Western Himalaya

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Picrorhiza kurrooa Royle ex Benth (Kutki or Kadvi) belonging to family Plantaginaceae is a valuable herbaceous plant. The target accessions of species were cultivated at Herbal Nursery (Pothivasa, 2200 masl.) of High Altitude Plant Physiology Research Centre (HAPPRC) Srinagar Garhwal (Uttarakhand) India, and the results obtained on the basis of high-performance thin-layer liquid chromatography (HPTLC) showed a varied range of picrosides content, i.e., Picroside-I (0.138–5.677%), Picroside-II (0.697–7.048%), and total picrosides (2.154–7.444%). The plant leaves (Picroside-I 4.54%; Picroside-II 1.32%) and stolons (Picroside-I 0.46; Picroside-II 5.52%) were proved as a potent source (average value) of picrosides content based on NJ and PCA analysis. The outcomes suggested that market demands might be fulfilled by the supply of leaves, which will be helpful for conservation of species and elite accession will be further used for multiplication. Therefore, mass-scale cultivation of P. kurrooa should be promoted. Cultivation of species will be helpful for reducing pressure on wild populations, meet up the market demand, develop employment opportunities, and conserve the biodiversity.

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Acknowledgements

The authors are thankful to the Director, High Altitude Plant Physiology Research Centre (HAPPRC), HNB Garhwal University (A Central University), Srinagar, Garhwal for facilities and encouragement. Financial support received from UGC (Fellowship) is gratefully acknowledged.

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Authors and Affiliations

  1. High Altitude Plant Physiology Research Centre (HAPPRC), H.N.B. Garhwal University, Srinagar (Garhwal), Uttarakhand, 246174, India

    Dharam Chand Attri, Mohan Chandra Nautiyal, Vijaylaxmi Trivedi & Jyoti Sati

  2. Zandu Foundation for Health Care, Ambach, Valsad, Gujarat, 396145, India

    Vinod Kumar Bisht

  3. Veer Chandra Singh Garhwali Uttarakhand University of Horticulture and Forestry, Bharsar, Pauri Garhwal, Uttarakhand, 246123, India

    Bhagwati Prasad Nautiyal

  4. G. B. Pant National Institute of Himalayan Environment (NIHE), Kosi-Katarmal, Almora, Uttarakhand, 263643, India

    Amit Bahukhandi

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  1. Dharam Chand Attri
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  2. Mohan Chandra Nautiyal
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  3. Vinod Kumar Bisht
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  4. Bhagwati Prasad Nautiyal
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Correspondence to Dharam Chand Attri or Mohan Chandra Nautiyal.

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Significance statement: The study revealed that cultivated accession of P. kurrooa is a good source of iridoid glycosides (picrosides). Therefore, to accomplish the market demand, large scale cultivation and utilization of plant leaves will be helpful for reducing the pressure on wild populations.

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Attri, D.C., Nautiyal, M.C., Bisht, V.K. et al. HPTLC Fingerprinting of Cultivated Picrorhiza kurrooa Accessions in Uttarakhand, Western Himalaya. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 91, 821–828 (2021). https://doi.org/10.1007/s40011-021-01286-3

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  • DOI: https://doi.org/10.1007/s40011-021-01286-3

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