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Evaluation of Ephedrine Content and Identification of Elite Chemotype(s) of Ephedra gerardiana (Wall.) from Kashmir Himalayas

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Abstract

Ephedra gerardiana Wall. (Ephedraceae) is an evergreen xerophytic shrub and is used in Indian and Chinese folk medicine since ancient times. It is used in asthma, hay fever, rheumatism, syphilis and as CNS stimulant due to its bioactive metabolite ephedrine. In this study, a validated HPTLC method was developed for the quantification and identification of the elite chemotype of E. gerardiana from Kashmir Himalayas. Ten samples were collected from different regions and their botanical and pharmacognostical standards were established. HPTLC method was developed and validated on pre-coated silica gel 60 GF254 plates in a tertiary solvent system of methylene chloride–ammonia–propan-2-ol (0.5:1.5:8 v/v), and quantification was done after derivatization with ninhydrin reagent. The method developed for quantification of ephedrine was linearly calibrated and found to be specific, repeatable and robust. LOD and LOQ were 36.33 and 110.10 µg/spot. Precision studies (inter-day and intra-day) are within the standard limit of ICH guidelines, and RSD (%) is not more than 5%. Mean recovery of ephedrine at three spiking levels of 25, 50 and 75% was 103.17%. The quantification of ephedrine within the population varies from 0.31 ± 0.05 to 0.81 ± 0.02% dry wt. basis, and in cluster analysis, two elite chemotypes viz. EPH-1 and EPH-5 were identified. These findings will help in setting the standard parameter for this plant in herbal Pharmacopoeia. The study will aid in the identification of potential sources of high ephedrine containing germplasm which will be useful to industry for quality raw material in herbal drug development.

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References

  1. Chauhan NS (1999) Medicinal and aromatic plants of Himachal Pradesh. Indus Publishing, Delhi

    Google Scholar 

  2. Polunin O, Stainton A, Farrer A (1987) Concise flowers of the Himalaya. Oxford University Press, London

    Google Scholar 

  3. Dollery C (1991) Therapeutic drugs. Churchill Livingstone Inc, New York

    Google Scholar 

  4. Bruneton J (1995) Pharmacognosy, phytochemistry, medicinal plants. Lavoisier Publishing, Paris

    Google Scholar 

  5. Dewick PM (2002) Medicinal natural products: a biosynthetic approach. Wiley, Hoboken

    Google Scholar 

  6. https://www.accessdata.fda.gov/drugsatfda_docs/nda/2017/208943Orig1s000ClinPharmR.pdf

  7. Khatoon S, Srivastava M, Rawat A, Mehrotra S (2005) HPTLC method for chemical standardization of Sida species and estimation of the alkaloid ephedrine. JPC-J Planar Chromatogr-Modern TLC 18(105):364–367

    Article  CAS  Google Scholar 

  8. Luhua Z, Fang Y, Lili Y, Bingren X (2005) Chromatographic separation of (−)-Ephedrine and (+)-Pseudoephedrine in the traditional Chinese medicinal preparation jiketing granule. Chem Pharm Bull 53(11):1494–1497

    Article  CAS  Google Scholar 

  9. Anonymous (2004) Ayurvedic pharmacopoeia of India, First edition: part 1, vol 4. Government of India, Department of Health, Ministry of Health and Family Welfare, New Delhi, pp 159–160

    Google Scholar 

  10. Anonymous (2009) Ayurvedic pharmacopoeia of India, First edition: part 2, vol 2. Government of India, Department of Health, Ministry of Health and Family Welfare, New Delhi, pp 166–167

    Google Scholar 

  11. Bray HG, Thorpe WV (1954) Analysis of phenolic compounds of interest in metabolism. Methods Biochem Anal 1:27–52

    CAS  PubMed  Google Scholar 

  12. Woisky RG, Salatino A (1998) Analysis of propolis: some parameters and procedures for chemical quality control. J Apic Res 37(2):99–105

    Article  CAS  Google Scholar 

  13. Anonymous (1984) Official methods of Analysis of Association of Official Analytical Chemists (AOAC), Virginia, US

  14. ICH guidelines Q2R1 (1996/2005) Validation of analytical procedures: text and methodology, Geneva, Switzerland. http://www.ich.org

  15. Rungsung W, Dutta S, Ratha KK, Mondal DN, Hazra J (2015) Pharmacognostical and phytochemical study on the stem of ephedra gerardiana. JIRMPS 2(3):80–85

    Google Scholar 

  16. Carneiro GRA, Silva AMS, Cavalcante RM, Padilha MC, Neto FRA, Pereira HMG, Sardela VF (2018) Fast ephedrine quantification by gas chromatography mass spectrometry. J Braz Chem Soc 29(12):2514–2521

    CAS  Google Scholar 

  17. Ibragic S, Sofić E (2015) Chemical composition of various Ephedra species. Bosn J Basic Med Sci 15(3):21–27

    Article  PubMed  PubMed Central  Google Scholar 

  18. Lv M, Chen J, Gao Y, Sun J, Zhang Q, Zhang M et al (2015) Metabolomics based on liquid chromatography with mass spectrometry reveals the chemical difference in the stems and roots derived from Ephedra sinica. J Sep Sci 38:3331–3336

    Article  CAS  PubMed  Google Scholar 

  19. Lv D, Cao Y, Lou Z, Li S, Chen X, Chai Y, Lu F (2015) Rapid on-site detection of ephedrine and its analogues used as adulterants in slimming dietary supplements by TLC-SERS. Anal Bioanal Chem 407:1313–1325

    Article  CAS  PubMed  Google Scholar 

  20. Xu J, Yan R (2017) Determination of the metabolite of ephedrine, 4-hydroxyephedrine, by LC–MS-MS in rat urine and its application in excretion profiles after oral administration of ephedra sinica Stapf and processing ephedra sinica Stapf. J Chromatogr Sci 55(2):162–165

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

The authors are thankful to the Director, CSIR-National Botanical Research Institute, Lucknow, for providing necessary facilities during the course of the experiment. They are also thankful to UGC, New Delhi, India for financial support (Ref. No. 843/(SC) (CSIR-UGC NET DEC. 2016)).

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Authors and Affiliations

  1. Pharmacognosy Division, CSIR-National Botanical Research Institute, Lucknow, U.P., 226001, India

    Mridul Kant Chaudhary, Ankita Misra & Sharad Srivastava

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  1. Mridul Kant Chaudhary
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  2. Ankita Misra
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  3. Sharad Srivastava
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Correspondence to Sharad Srivastava.

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Significance’ Statement

The present study is to evaluate microscopic characters, pharmacognostical standards and quantification ephedrine in Ephedra gerardiana through HPTLC and to identify the elite chemotype in Ephedra gerardiana collected from Kashmir Himalayas.

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Chaudhary, M.K., Misra, A. & Srivastava, S. Evaluation of Ephedrine Content and Identification of Elite Chemotype(s) of Ephedra gerardiana (Wall.) from Kashmir Himalayas. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 90, 833–841 (2020). https://doi.org/10.1007/s40011-019-01153-2

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  • DOI: https://doi.org/10.1007/s40011-019-01153-2

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