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Optimization of the Formulation and Preparation of Andrographolide Microemulsion by Box–Behnken Response Surface Methodology

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Abstract

Andrographis paniculata is a bitter herb used in Asian countries to treat inflammation. Andrographolide (AG) is a diterpenoid compound extracted from Andrographolide paniculata. It is the main active component of Andrographolide paniculata. However, due to its low solubility in water, its biological use is extremely low and it is not easily absorbed during cervical recovery, greatly limiting its application in edible beverages. In this study, emulsifiers and co-emulsifiers were used to miniaturize the andrographolide extracted solution and the process system was optimized by response surface methodology. The AG oil solution was a light yellow transparent liquid; the HLB value was 12.2, which met the requirements of self-emulsification to form O/W micro-emulsion after emulsification. The stability of the optimized AG oil solution showed that the appearance of the solution was light yellow, clear and transparent, and unchanged, and the drug content remained stable at normal temperature and natural conditions for 0, 1, 3 and 6 months.

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References

  1. Rajagopal S, Kumar RA, Deevi DS, Satyanarayana C, Rajagopalan R (2003) Andrographolide, a potential cancer therapeutic agent isolated from andrographis paniculata. J Exp Ther Oncol 3(3):147–158. https://doi.org/10.1046/j.1359-4117.2003.01090.x

    Article  CAS  PubMed  Google Scholar 

  2. Patil R, Jain V (2021) Andrographolide: a review of analytical methods. J Chromatogr Sci 59:119–203. https://doi.org/10.1093/chromsci/bmaa091

    Article  CAS  Google Scholar 

  3. Zhang L, Bao M, Liu B, Zhao H, Lu C (2020) Effect of andrographolide and its analogs on bacterial infection. J Chromatogr Sci 105(3–4):1–12. https://doi.org/10.1159/000503410

    Article  CAS  Google Scholar 

  4. Banerjee A, Ahmed H, Yang P, Czinn SJ, Blanchard TG (2016) Endoplasmic reticulum stress and ire-1 signaling cause apoptosis in colon cancer cells in response to andrographolide treatment. Oncotarget 7(27):41432–41444. https://doi.org/10.18632/oncotarget.9180

    Article  PubMed  PubMed Central  Google Scholar 

  5. Aishwarya B, Sathiyanarayanan L, Arulmozhi S, Kakasaheb M (2017) Herb-drug interaction of andrographis paniculata (nees) extract and andrographolide on pharmacokinetic and pharmacodynamic of naproxen in rats. J Ethnopharmacol 195:214–221. https://doi.org/10.1016/j.jep.2016.11.022

    Article  CAS  Google Scholar 

  6. Sharma V, Sharma T, Kaul S, Kapoor KK, Dhar MK (2017) Anticancer potential of labdane diterpenoid lactone “andrographolide” and its derivatives: a semi-synthetic approach. Phytochem Rev. https://doi.org/10.1007/s11101-016-9478-9

    Article  Google Scholar 

  7. Sanad AB, Abdel-Bar HM (2017) Chitosan–hyaluronic acid composite sponge scaffold enriched with andrographolide-loaded lipid nanoparticles for enhanced wound healing. Carbohydrate Polym 173:441–450. https://doi.org/10.1016/j.carbpol.2017.05.098

    Article  CAS  Google Scholar 

  8. Cametti C, Codastefano P, Tartaglia P, Rouch J, Chen SH (1990) Theory and experiment of electrical conductivity and percolation locus in water-in-oil microemulsions. Phys Rev Lett 64(12):1461–1464. https://doi.org/10.1103/PhysRevLett.64.1461

    Article  ADS  CAS  PubMed  Google Scholar 

  9. Bordi F, Cametti C, Chen SH, Rouch J, Sciortino F, Tartaglia P (1996) The static electrical conductivity of water-in-oil microemulsions below percolation threshold. Phys A Stat Mech Appl 231(1–3):161–167. https://doi.org/10.1016/0378-4371(96)00080-5

    Article  CAS  Google Scholar 

  10. Yerramilli M, Longmore N, Ghosh S (2018) Stability and bioavailability of curcumin in mixed sodium caseinate and pea protein isolate nanoemulsions. J Am Oil Chem Soc. https://doi.org/10.1002/aocs.12084

    Article  Google Scholar 

  11. Araiza-Calahorra A, Akhtar M, Sarkar A (2018) Recent advances in emulsion-based delivery approaches for curcumin: from encapsulation to bioaccessibility. Trends Food Sci Technol 71:155–169. https://doi.org/10.1016/j.tifs.2017.11.009

    Article  CAS  Google Scholar 

  12. Costa P, Pereira RC (2019) Phase diagram, scalar-pseudoscalar meson behavior and restoration of symmetries in (2+1) polyakov-nambu-jona-lasinio model. Symmetry 11(4):507. https://doi.org/10.3390/sym11040507

    Article  ADS  CAS  Google Scholar 

  13. Yang Y, Li X, Xuan Z, Chen Z, Zhou Q, Yong C (2018) Superhydrophobicity: 3d-printed biomimetic super-hydrophobic structure for microdroplet manipulation and oil/water separation. Adv Mater 30(9):1704912. https://doi.org/10.1002/adma.201704912

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported by the Commercial Ministry of Democratic People’s Republic of Korea (CF No. 3123416), the Science Foundation of DPRK (SU No. 980961C) and the Education Ministry of DPRK (ED No. KJ2016A061).

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Authors and Affiliations

  1. Department of Foreign Cooking, Faculty of Catering, Pyongyang Jang Chol Gu University of Commerce, Pyongyang, Korea

    KiBong Kim, SongRok Uh, IlJin Kim, KiSun Ri, KilNam Sin & YongSang Kim

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  1. KiBong Kim
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  2. SongRok Uh
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  3. IlJin Kim
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  4. KiSun Ri
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All authors either performed the assays or analyzed the data of their own laboratory; all authors approved the final version of the manuscript.

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Correspondence to KiBong Kim.

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Kim, K., Uh, S., Kim, I. et al. Optimization of the Formulation and Preparation of Andrographolide Microemulsion by Box–Behnken Response Surface Methodology. Natl. Acad. Sci. Lett. 46, 479–481 (2023). https://doi.org/10.1007/s40009-023-01228-9

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