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High Frequency Organogenesis in Cotyledon and Hypocotyl Explants of Cabbage (Brassica oleracea L. var. capitata)

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Abstract

Morphogenetic potential of cotyledon and hypocotyl explants of cabbage (Brassica oleracea L. var. capitata) was studied to develop a reliable plant regeneration protocol. Nine days old aseptically grown seedlings of cabbage were used as source of explant for plant regeneration. Out of 36 combinations of growth regulators used in MS medium, high frequency shoot regeneration from cotyledon explant was obtained on MS medium supplemented with 1.5 mg/l BAP and 0.50 mg/l NAA and 2 mg/l BAP and 0.25 mg/l IAA respectively. High percentage root regeneration (90 %) in in vitro developed shoots were obtained on MS medium supplemented with 0.10 mg/l IBA. The regenerated complete plantlets were transferred to pots containing a mixture of sand and soil and acclimatized. A reproducible and efficient plant regeneration protocol has been standardized in cabbage cv. Pride of India.

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Abbreviations

BAP:

N 6-Benzylaminopurine

NAA:

Naphthalene acetic acid

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

MS:

Murashige and Skoog

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Acknowledgments

Authors thank to Professor and Head, Dept. of Vegetable Science of Dr. Y.S. Parmar University of Horticulture and Forestry, Solan, India for providing germplasm of cabbage cv. Pride of India.

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Authors and Affiliations

  1. Dept. of Biotechnology, Institute of Life Sciences and Business Management, Solan, Himachal Pradesh, India

    Shalini Sharma

  2. Department of Biotechnology, Dr Y.S. Parmar University of Horticulture and Forestry Nauni, Solan, 173230, Himachal Pradesh, India

    Shalini Sharma, Geetika Gambhir & D. K. Srivastava

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  1. Shalini Sharma
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  2. Geetika Gambhir
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  3. D. K. Srivastava
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Correspondence to D. K. Srivastava.

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Sharma, S., Gambhir, G. & Srivastava, D.K. High Frequency Organogenesis in Cotyledon and Hypocotyl Explants of Cabbage (Brassica oleracea L. var. capitata). Natl. Acad. Sci. Lett. 37, 5–12 (2014). https://doi.org/10.1007/s40009-013-0191-6

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  • DOI: https://doi.org/10.1007/s40009-013-0191-6

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