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Electrophoretic Characterization of Sanitarily and Medically Important Flesh Fly, Sarcophaga dux (Diptera, Sarcophagidae) Using Isozymes

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Abstract

Sarcophagid flies are an important group of Dipteran flies and are known to transmit pathogenic bacteria and viruses and cause animal tissue myiasis. These flies have also been reported as agents of human cutaneous, intestinal and genitourinal myiasis. However there is very little information available on the biochemical genetics of these flies. So the present investigation is an attempt to analyse the electrophoretic profiles using molecular markers in Sarcophaga dux by PAGE at five enzyme loci [(Malic enzyme (ME), Acid phosphatase (ACPH), Alkaline phosphatase (APH), Lactate dehydrogenase (LDH) and Xanthine dehydrogenase (XDH)]. All the enzymes were found to be encoded at a single locus. Of these, ME and XDH were monomorphic. While LDH, APH and ACPH revealed polymorphism for two electromorphs and three electrophoretic phenotypes.

Graphical Abstract

Electrophoretic banding patterns at five enzyme loci i.e., ACPH, APH, LDH, ME and XDH were studied in S. dux. All the five enzymes were found to be encoded at a single locus. Of these, ME and XDH were monomorphic. While LDH, APH and ACPH revealed polymorphism for two electromorphs and three electrophoretic phenotypes.

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Authors and Affiliations

  1. Department of Biological Sciences, Sam Higginbottom Institute of Agriculture, Technology & Sciences, Deemed University, Allahabad, 211007, India

    Kiran Singh & Sasya Thakur

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  1. Kiran Singh
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  2. Sasya Thakur
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Correspondence to Sasya Thakur.

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Singh, K., Thakur, S. Electrophoretic Characterization of Sanitarily and Medically Important Flesh Fly, Sarcophaga dux (Diptera, Sarcophagidae) Using Isozymes. Natl. Acad. Sci. Lett. 35, 339–342 (2012). https://doi.org/10.1007/s40009-012-0024-z

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  • DOI: https://doi.org/10.1007/s40009-012-0024-z

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