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Development of topical natural based film forming system loaded propolis from stingless bees for wound healing application

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Abstract

Purpose

Propolis extract is an extraction of a resinous mixture of honey bees produced by mixing saliva and beeswax with exudate gathered from tree. The prominent pharmacological activities of propolis extract are antioxidant, antimicrobial, fungicidal, antiviral, antiulcer, immune-stimulating, and cytostatic, which are very suitable for topical formulation.

Methods

From its various pharmacological activities, propolis extract was formulated in film forming system using modified tamarind seed gum as a solution/gel/film forming agent. To obtain the optimum seed gum concentration of the film forming system (solution to film), various tamarind concentrations and solvent ratios were evaluated.

Results

The formulation of 7.5% of modified tamarind seed gum with 1% PEG400 dissolved in 55%:45% of water:ethanol provided fast drying time (4.36 min) and proper rheological property for spaying application. The propolis extract loaded formulation could inhibit Staphylococcus aureus and Staphylococcus epidermidis. Moreover, the product satisfaction evaluation was excellent (3.8 from 5).

Conclusion

The results indicate the possibility of introducing modified tamarind seed gum and propolis extract as a component in the development of film forming systems and increasing the effectiveness of antibacterial on the skin.

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Acknowledgements

The authors acknowledge the Faculty of Pharmaceutical Sciences, Burapha University for financial support (Grant Number Rx1/2563). The assistance of Ms.Thamonwan Choumthong, Ms. Pin Topothai, and Ms. Bussarakum Rungjang on laboratory work is greatly appreciated. The authors also thank Dr. Wancheng Sittikijyothin and Dr. Boonyadist Vongsak for tamarind seed gum and stingless bee propolis support, respectively. The authors would like to thank Enago (www.enago.com) for the English language review.

Funding

This study was funded by the Faculty of Pharmaceutical Sciences, Burapha University (Grant Number Rx1/2563).

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Burapha University, 169, Saensook, Muang, Chonburi, 20131, Thailand

    Kampanart Huanbutta & Tanikan Sangnim

  2. Department of Chemical Engineering, Faculty of Engineering, Burapha University, Chonburi, 20131, Thailand

    Wancheng Sittikijyothin

Authors
  1. Kampanart Huanbutta
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  2. Wancheng Sittikijyothin
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  3. Tanikan Sangnim
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Correspondence to Tanikan Sangnim.

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The authors declare that they have no conflict of interest.

Statement of human and animal rights

The study protocol was approved by the institutional review board of Burapha University (No. 20/2561). Informed consent was confirmed by the IRB.

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Huanbutta, K., Sittikijyothin, W. & Sangnim, T. Development of topical natural based film forming system loaded propolis from stingless bees for wound healing application. J. Pharm. Investig. 50, 625–634 (2020). https://doi.org/10.1007/s40005-020-00493-w

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  • DOI: https://doi.org/10.1007/s40005-020-00493-w

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