Abstract
Contents of phenolic compound including isoflavones and phenolic acids as well as antioxidant effects in high-protein soybean cultivar “Saedanbaek” were evaluated under different roasting conditions. The roasted soybean exhibited significantly higher antioxidant activity than unroasted soybean in the three antioxidant methods including 2,2-diphenyl-1-picrylhydrazyl, 2,2-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid), and Ferric reducing antioxidant power. In particular, the roasted soybean at 200°C for 15 min showed the highest antioxidant activity in comparison with other conditions. The contents of phenolic compounds, isoflavone aglycones (genistein, daidzein, and glycitein), isoflavone β-glucosides (genistin, daidzin, and glycitin), and phenolic acids increased, whereas isoflavone malonyl-β-glucosides decreased during roasting process. Moreover, total phenolic and flavonoid contents as well as those of isoflavone aglycones, isoflavone-β-glucosides, and phenolic acids increased, leading to a general increase in antioxidant activity after roasting. These results suggest that the roasting soybean extracts could contribute to obtaining natural antioxidants in certain food applications.
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Alam MN, Bristi NJ, and Rafiguzzaman M (2013) Review on in vivo and vitro methods evaluation of antioxidant activity. Saudi Pharm J 21, 143–152.
Bolis MS (1958) Anitoxidant determination by the use of a stable free radical. Nature 181, 1199–1200.
Chandrasekara N and Shahidi F (2011) Antioxidative potential of cashew phenolics in food and biological model systems as affected by roasting. Food Chem 129, 1388–1396.
Cho KM, Hong SY, Math RK, Lee JH, Kambiranda DM, Kim JM et al. (2009) Biotransformation of phenolics (isoflavones, flavanols and phenolic acids) during the fermentation of cheonggukjang by Bacillus pumilus HY1. Food Chem 114, 413–419.
Cho KM, Lee JH, Yun HD, Ahn BY, Kim H, and Seo WT (2011) Changes of phytochemical constituents (isoflavones, flavanols, and phenolic acids) during cheonggukjang soybeans fermentation using potential probiotics Bacillus subtilis CS90. J. Food Comp Anal 24, 402–410.
Choi JS, Kim HY, Seo WT, Lee JH, and Cho KM (2012) Roasting enhances antioxidant effect of bitter melon (Momordica charantia L.) increasing in flavan-3-ol and phenolic acid contents. Food Sci Biotechnol 21, 19–26.
Chung IM, Seo SH, Ahn JK, and Kim SH (2011) Effect of processing, fermentation, and aging treatment to content and profile of phenolic compounds in soybean seed, soy curd and soy paste. Food Chem 127, 960–967.
Coward L, Smith M, Kirk M, and Barnes S (1998) Chemical modification of isoflavones in soyfoods during cooking and processing. American J Clin Nutr 68, 1486–1491.
Dewanto V, Wu X, and Liu RH (2002) Processed sweet corn has higher antioxidant activity. J Agric Food Chem 50, 4959–4964.
Durmaz G and Gökmen V (2011) Changes in oxidative stability, antioxidant capacity and phytochemical composition of Pistacia terebinthus oil with roasting. Food Chem 128, 410–414.
Gallegos-Infante JA, Rocha-Guzman NE, Gonzalez-Laredo RF, and Pulido-Alonso J (2010) Effect of processing on the antioxidant properties of extracts Mexican barley (Hordeum vulgare) cultivar. Food Chem 119, 903–906.
Im MH, Choi JD, and Choi KS (1995) The oxidation stability and flavor acceptability of oil from roasted soybean. J. Agric Food Chem Biotechnol 38, 425–430.
John JA and Shahidi F (2010) Phenolic compounds and antioxidant activity of Brazil nut (Bertholleta excels). J Funct Foods 2, 196–209.
Jung S, Murphy, PA, and Sala I (2008) Isoflavone profiles of soymilk as affected by high-pressure treatments of soymilk and soybeans. Food Chem 111, 592–598.
Kao TH and Chen BH (2006) Functional components in soybean cake and their effects on antioxidant activity. J. Agric Food Chem 54, 7544–7555.
Kim HG, Kim GW, Oh H, Yoo SY, Kim YO, and Oh MS (2011) Influence of roasting on the antioxidant activity of small black soybean (Glycine max L. Merrill). LWT-Food Sci Technol 44, 992–998.
Kwak CS, Lee MS, and Park SC (2007) Higher antioxidant properties of chungkookjang, a fermented soybean paste, may be due to increased aglycone and malonylglycoside isoflavone during fermentation. Nutr Res 27, 719–727.
Lee CH, Yang L, Xu JZ, Yeung SYV, Huang Y, and Chen ZY (2005) Relative antioxidant activity of soybean isoflavones and their glycosides. Food Chem 90, 735–741.
Lee JH, Baek IY, Ko JM, Kang NS, Shin SH, Lim SG et al. (2008) Antioxidant and tyrosinase inhibitory activities from seed coat of brown soybean. Food Sci Biotechnol 17, 1–7
Lee JH and Cho KM (2012) Changes occurring in compositional components of black soybeans maintained at room temperature for different storage periods. Food Chem 131, 161–169.
Lee JH and Choung MG (2011) Determination of optimal acid hydrolysis time of soybean isoflavones using drying oven and microwave assisted methods. (2011) Food Chem 129, 577–582.
Lee JH, Seo WT, Lim WJ, and Cho KM (2011) Phenolic contents and antioxidant activities from different tissues of baekseohyang (Daphne kiusiana). Food Sci Biotechnol 20, 695–702.
Lee SW and Lee JH (2009) Effects of oven-drying, roasting, and explosive puffing process on isoflavone distributions in soybeans. Food Chem 112, 316–320.
Locatelli M, Travaglia F, Coïsson JD, Martelli A, Stévigny C, and Arlorio M (2010) Total antioxidant activity of hazelnut skim (Nocciola Piemonte PGI): Impact of different roasting conditions. Food Chem 119, 1647–1655.
Malencic D, Popovic M, and Miladinovic J (2007) Phenolic content and antioxidant properties of soybean (Glycine max (L.) Merr.) seeds. Molecules 12, 576–581.
Manzocco L, Calligaris S, Mastrocola D, Nicoli MC, and Lerici CR (2000) Review of non-enzymatic browning and antioxidant capacity in processed foods. Trend Food Sci Biotechnol 11, 340–346.
Oliviero T, Capuano E, Cammerer B, and Fogliano V (2009) Influence of roasting on the antioxidant activity and HMF formation of a cocoa bean model systems. J Agric Food Chem 57, 147–152.
Riedl KM, Lee JH, Renita M, St Martin SK, Schwartz SJ, and Vodovotz Y (2007). Isoflavone profiles, phenol content, and antioxidant activity of soybean seeds as influenced by cultivar and growing location in Ohio. J Sci Food Agric 87, 1197–1206.
Saulnier L, Marto C, Elgorriaga M, Bonnin E, and Thibault JF (2001) Thermal and enzymatic treatments for the release of free ferulic acid from maize bran. Carbohyd Polym 45, 269–275.
Shimoni E (2004) Stability and shelf life of bioactive compounds during food processing and storage: soy isoflavone. J Food Sci 69, 160–166.
Shon MY, Lee J, Choi SY, Nam SH, Seo KI, Lee SW et al. (2007) Antioxidant and free radical scavenging activity of methanol extract of chungkukjang. J Food Comp Anal 20, 113–118
Singh S and Singh RP (2008) In vitro methods of assay of antioxidant: an overview. Food Rev Int 24, 392–415.
Slavin M, Cheng Z, Luther M, Kenworthy W, and Yu L. (2009). Antioxidant properties and phenolic, isoflavone, tocopherol and carotenoid composition of Maryland-grown soybean lines with altered fatty acid profiles. Food Chem 114, 20–27.
Toda T, Sakamoto A, Takayanagi T, and Yokotsuka Y (2000) Changes in isoflavone compositions of soybean foods during cooking process. Food Sci Technol Res 6, 314–319.
Yang EJ, Kim SI, Hur JM, and Song KS (2009) Roasting process enhances antioxidative effect of cinnamon (Cinnamoni cortex) via increase in cinnamaldehyde content. J Korean Soc Appl Biol Chem 52, 443–447.
Youn KS and Chung HS (2012) Optimization of the roasting temperature and time for preparation of coffee-like maize beverage using the response surface methodology. LWT-Food Sci Technol 46, 305–310.
Xu Z, Wu Q, and Godber JS, (2002) Stabilities of daidzin, glycitin, genistin, and generation of derivatives during heating. J Agric Food Chem 50, 7402–7406.
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Lee, J.H., Lee, B.W., Kim, B. et al. Changes in phenolic compounds (Isoflavones and Phenolic acids) and antioxidant properties in high-protein soybean (Glycine max L., cv. Saedanbaek) for different roasting conditions. J Korean Soc Appl Biol Chem 56, 605–612 (2013). https://doi.org/10.1007/s13765-013-3048-2
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DOI: https://doi.org/10.1007/s13765-013-3048-2