Abstract
The inhibitory effects of resveratrol, a natural bioactive polyphenolic phytoalexin rich in grape, on lipopolysaccharide (LPS)-induced microglial activation and its beneficial effects on dopaminergic neurodegeneration were studied. Resveratrol dosedependently suppressed LPS-induced nitric oxide production and the expression of inducible NO synthase (iNOS) in BV-2 microglial cells. Furthermore, resveratrol (1 μM) significantly blocked BV2 microglial phagocytosis induced by LPS (0.1 μg/mL). Although the conditioned media from LPS-stimulated BV2 cells caused the SN4741 dopaminergic neuronal cell death, that from resveratrolpretreated BV2 cells did not diminish the viability of SN4741 cells. These results suggest that resveratrol can prevent neuronal death possibly through the modulation of phagocytosis and microglial activation.
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References
Abraham J and Johnson RW (2009) Consuming a diet supplemented with resveratrol reduced infection-related neuroinflammation and deficits in working memory in aged mice. Rejuvenation Res 12, 445–453.
Banati RB, Daniel SE, and Blunt SB (1998) Glial pathology but absence of apoptotic nigral neurons in long-standing Parkinson’s disease. Mov Disord 13, 221–227.
Baur JA and Sinclair DA (2006) Therapeutic potential of resveratrol: the in vivo evidence. Nat Rev Drug Discov 5, 493–506.
Bhat KPL, Kosmeder JWII, and Pezzuto JM (2001) Biological effects of resveratrol. Antioxid Redox Signal 3, 1041–1064.
Bi XL, Yang JY, Dong YX, Wang JM, Cui YH, Ikeshima T et al. (2005) Resveratrol inhibits nitric oxide and TNF-alpha production by lipopolysaccharide-activated microglia. Int Immunopharmacol 5, 185–193.
Blasi E, Barluzzi R, Bocchini V, Mazzolla R, and Bistoni F (1990) Immortalization of murine microglial cells by a v-raf/v-myc carrying retrovirus. J Neuroimmunol 27, 229–237.
Borra MT, Smith BC, and Denu JM (2005) Mechanism of human SIRT1 activation by resveratrol. J Biol Chem 280, 17187–17195.
Burns J, Yokota T, Ashihara H, Lean ME, and Crozier A (2002) Plant foods and herbal sources of resveratrol. J Agric Food Chem 50, 3337–3340.
Candelario-Jalil E, de Oliveira AC, Gräf S, Bhatia HS, Hüll M, Muñoz E et al. (2007) Resveratrol potently reduces prostaglandin E2 production and free radical formation in lipopolysaccharide-activated primary rat microglia. J Neuroinflammation 4, 25.
Chen J, Zhou Y, Mueller-Steiner S, Chen LF, Kwon H, Yi S et al. (2005) SIRT1 protects against microglia-dependent amyloid-beta toxicity through inhibiting NF-kappaB signaling. J Biol Chem 280, 40364–40374.
Chun HS and Low WC (2012) Ursodeoxycholic acid suppresses mitochondria-dependent programmed cell death induced by sodium nitroprusside in SH-SY5Y cells. Toxicology 292, 105–112.
Chun HS, Gibson GE, DeGiorgio LA, Zhang H, Kidd VJ, and Son JH (2001) Dopaminergic cell death induced by MPP(+), oxidant and specific neurotoxicants shares the common molecular mechanism. J Neurochem 76, 1010–1021.
Chung S, Yao H, Caito S, Hwang JW, Arunachalam G, and Rahman I (2010) Regulation of SIRT1 in cellular functions: role of polyphenols. Arch Biochem Biophys 501, 79–90.
Corradin SB, Mauël J, Donini SD, Quattrocchi E, and Ricciardi-Castagnoli P (1993) Inducible nitric oxide synthase activity of cloned murine microglial cells. Glia 7, 255–262.
Herrera AJ, Castaño A, Venero JL, Cano J, and Machado A (2000) The single intranigral injection of LPS as a new model for studying the selective effects of inflammatory reactions on dopaminergic system. Neurobiol Dis 7, 429–447.
Jin F, Wu Q, Lu YF, Gong QH, and Shi JS (2008) Neuroprotective effect of resveratrol on 6-OHDA-induced Parkinson’s disease in rats. Eur J Pharmacol 14, 78–82.
Kim YS and Joh TH (2006) Microglia, major player in the brain inflammation: their roles in the pathogenesis of Parkinson’s disease. Exp Mol Med 38, 333–347.
Kim WG, Mohney RP, Wilson B, Jeohn GH, Liu B, and Hong JS (2000) Regional difference in susceptibility to lipopolysaccharide-induced neurotoxicity in the rat brain: role of microglia. J Neurosci 20, 6309–6316.
Laurenzi MA, Arcuri C, Rossi R, Marconi P, and Bocchini V (2001) Effects of microenvironment on morphology and function of the microglial cell line BV-2. Neurochem Res 26, 1209–1216.
McGeer PL and McGeer EG (2008) Glial reactions in Parkinson’s disease. Mov Disord 23, 474–483.
Orr CF, Rowe DB, and Halliday GM (2002) An inflammatory review of Parkinson’s disease. Prog Neurobiol 68, 325–340.
Sugama S, Cho BP, Degiorgio LA, Shimizu Y, Kim SS, Kim YS et al. (2003) Temporal and sequential analysis of microglia in the substantia nigra following medial forebrain bundle axotomy in rat. Neuroscience 116, 925–933.
Zhang F, Shi JS, Zhou H, Wilson B, Hong JS, and Gao HM (2010) Resveratrol protects dopamine neurons against lipopolysaccharideinduced neurotoxicity through its anti-inflammatory actions. Mol Pharmacol 78, 466–477.
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Park, E., Kim, D.K. & Chun, H.S. Resveratrol inhibits lipopolysaccharide-induced phagocytotic activity in BV2 cells. J Korean Soc Appl Biol Chem 55, 803–807 (2012). https://doi.org/10.1007/s13765-012-2156-8
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DOI: https://doi.org/10.1007/s13765-012-2156-8