Abstract
Bioinsecticides are important in the control of disease vectors, but data regarding their physiological effects on target insects are incomplete. This study describes morphological changes that occur in the midgut of third instar Aedes aegypti L. (Diptera: Culicidae) following treatment with a methanolic extract of Annona coriacea (Magnoliales: Annonaceae). Dissected midguts were subdivided into anterior and posterior regions and analyzed by light and scanning electron microscopy. Insects exposed to the extract displayed intense, destructive cytoplasmic vacuolization in columnar and regenerative midgut cells. The apical surfaces of columnar cells exhibited cytoplasmic protrusions oriented toward the lumen, suggesting that these cells could be involved in apocrine secretory processes and/or apoptosis. We report that A. coriacea extracts induced morphological alterations in the midgut of A. aegypti midgut larvae, supporting the use of plant extracts for control of the dengue vector.
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Acknowledgments
The authors would like to thank Dr. P. Pimenta (Centro de Pesquisa René Rachou—Fiocruz) for providing eggs of A. aegypti for the initial rearing, Dr. P. Teixeira and Dr. E. L. Dall'Oglio (Lab de Pesquisa em Química de Produtos Naturais—UFMT) for the preparation of the crude extract, and J.F.S. Cossolin and F. B. Magalhães for their support during the review of this paper. We also thank the Biological Sciences of IB-UNESP Botucatu, SP for their technical assistance with the scanning electron microscopy and BioMed Proofreading for English review. Financial support was provided by FAPEMAT.
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Edited by Roberto Romani – University of Perugia
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Costa, M., Pinheiro, D., Serrão, J. et al. Morphological Changes in the Midgut of Aedes aegypti L. (Diptera: Culicidae) Larvae Following Exposure to an Annona coriacea (Magnoliales: Annonaceae) Extract. Neotrop Entomol 41, 311–314 (2012). https://doi.org/10.1007/s13744-012-0050-z
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DOI: https://doi.org/10.1007/s13744-012-0050-z