Skip to main content
SpringerLink
Log in

Stability-indicating chromatographic and UV spectrophotometric methods for determination of colchicine

  • Original Paper
  • Published:
Journal of the Iranian Chemical Society Aims and scope Submit manuscript

Abstract

Colchicine is an alkaloid that used in the relief of acute gout. Three simple, sensitive, selective and rapid methods have been developed for the determination of colchicine in the presence of its alkaline degradation product with good applicability on its pharmaceutical formulation. The first method was based on TLC separation of colchicine and its degradation using chloroform/methanol/conc ammonia (8:2:0.1 v/v/v) as a mobile phase and densitometric detection at 350 nm. The second method was HPLC using kromasil C18 column and a mobile phase of methanol/ammonia (100:1.5 v/v) with UV detection at 246 nm. The third method was spectrophotometry which involved induced dual wavelength method at 245 nm and 261 nm and first derivative of ratio spectra at 302.4 nm. The developed methods were validated according to ICH guidelines and successfully applied for the determination of colchicine in tablets.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10
Fig. 11

Similar content being viewed by others

References

  1. U.S. Pharmacopoeia 29th edn. (U.S. Pharmacopeial Convention,Inc., Rockville, 2006)

  2. S.C. Sweetman, B. Pharm, F.R. Pharm, S. Martindale, The CompleteDrug Reference, 39th edn. (The Pharmaceutical Press, London, 2017)

    Google Scholar 

  3. S.A. Joshi, S.S. Jalalpure, A.A. Kempwade, M.R. Peram, Curr. Pharm. Anal. 14, 76–83 (2018)

    CAS  Google Scholar 

  4. K. Nirmala, R.R. Raju, Rasayan. J. Chem. 5, 106–111 (2012)

    CAS  Google Scholar 

  5. B.G. Gowda, Int. J. Pharm. Pharm. Sci. 6, 335–337 (2014)

    CAS  Google Scholar 

  6. V.F. Samanidou, G.A. Sarantis, I.N. Papadoyannis, J. Liq. Chromatogr. Relat. Technol. 29, 1–13 (2006). https://doi.org/10.1080/10826070500357755

    Article  CAS  Google Scholar 

  7. V. Maslarska, I. Pencheva, Int. J. Adv. Pharm. Biol. Chem. 3, 116–119 (2014)

    CAS  Google Scholar 

  8. I.M. Abdulbaqi, Y. Darwis, N. Khan, G.O.K. Loh, Int. J. Pharm. Pharm. Sci. 9, 72–78 (2017). https://doi.org/10.22159/ijpps.2017v9i7.18336

    Article  CAS  Google Scholar 

  9. F.K. Alanazi, A. Sc. J. Az. Med. Fac (Girls) 28, 987–997 (2007)

    Google Scholar 

  10. G.M. Hadad, J.M. Badr, K. El-Nahriry, H.A. Hassanean, J. Chromatogr. Sci. 51, 258–265 (2013). https://doi.org/10.1093/chromsci/bms135

    Article  CAS  PubMed  Google Scholar 

  11. T.M. Sarg, M.M. El-Domiaty, M.M. Bishr, Analyst 114, 575–578 (1989). https://doi.org/10.1039/an9891400575

    Article  CAS  PubMed  Google Scholar 

  12. E. Bodoki, R. Oprean, L. Vlase, M. Tamas, R. Sandulescu, J. Pharm. Biomed. Anal. 37, 971–977 (2005). https://doi.org/10.1016/j.jpba.2004.10.006

    Article  CAS  PubMed  Google Scholar 

  13. V. Mirakor, V. Vaidya, S. Menon, A. Laud, J. Planar. Chromat. 21, 187–189 (2008). https://doi.org/10.1556/JPC.21.2008.3.6

    Article  CAS  Google Scholar 

  14. N. Soni, P. Patidar, D. Dubey, K. Dashora, J. Pharm. Res. 5, 3858–3859 (2012)

    CAS  Google Scholar 

  15. S.A. Mohammed, S.A. Abd El-Razeq, I.A. Mohammed, Asian J. Appl. Chem. Res. 2, 1–12 (2018). https://doi.org/10.9734/ajacr/2018/v2i229673

    Article  Google Scholar 

  16. A.I. Mohamed, M.A. Omar, M. Hammad, A.A. Mohamed, Open Chem. 16, 496–510 (2018). https://doi.org/10.1515/chem-2018-0050

    Article  CAS  Google Scholar 

  17. R.K. Ajage, V.S. Kasture, Der. Pharm. Lett. 6, 160–166 (2014)

    CAS  Google Scholar 

  18. P. Verma, A. Patial, Int. Res. J. Pharm. 3, 87–89 (2012)

    Google Scholar 

  19. A. Daneshfar, F. Ansari, S. Hasanvandi, Orient. J. Chem. 28, 89–96 (2012). https://doi.org/10.13005/ojc/280113

    Article  CAS  Google Scholar 

  20. H. Zhang, Bioelectrochemistry 68, 197–201 (2006). https://doi.org/10.1016/j.bioelechem.2005.07.001

    Article  CAS  PubMed  Google Scholar 

  21. E. Bodoki, R.S. Andulescu, L. Roman, Cent. Eur. J. Chem. 5, 766–778 (2007). https://doi.org/10.2478/s11532-007-0034-8

    Article  CAS  Google Scholar 

  22. F. Wang, J. Zhou, Y. Liu, S. Wu, G. Song, B. Ye, Analyst 136, 3943–3949 (2011). https://doi.org/10.1039/c1an15372b

    Article  CAS  PubMed  Google Scholar 

  23. D.M. Stanković, L. Švorc, J.F. Mariano, A. Ortner, K. Kalcher, Electroanalysis 29, 2276–2281 (2017)

    Article  Google Scholar 

  24. ICH Validation of analytical procedures: text and methodology Q2 (R1), in International Conference on Harmonization (2005)

  25. H.M. Lotfy, S.S. Saleh, N.Y. Hassan, H. Salem, Spectrochim. Acta A Mol. Biomol. Spectrosc. 136, 1786–1796 (2015). https://doi.org/10.1016/j.saa.2014.10.084

    Article  CAS  PubMed  Google Scholar 

  26. M.S. Eissa, A.M. Abou Al Alamein, Spectrochim. Acta A Mol. Biomol Spectrosc. 193, 365–374 (2018). https://doi.org/10.1016/j.saa.2017.12.050

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Analytical Chemistry Department, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, Egypt

    Sawsan A. Abdel Razeq & Israa A. Khalil

  2. National Organization for Drug Control and Research (NODCAR), Giza, Egypt

    Samah A. Mohammed

Authors
  1. Sawsan A. Abdel Razeq
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Israa A. Khalil
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Samah A. Mohammed
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Israa A. Khalil.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Abdel Razeq, S.A., Khalil, I.A. & Mohammed, S.A. Stability-indicating chromatographic and UV spectrophotometric methods for determination of colchicine. J IRAN CHEM SOC 17, 2415–2427 (2020). https://doi.org/10.1007/s13738-020-01937-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13738-020-01937-8

Keywords

Search

Navigation