Skip to main content

Advertisement

SpringerLink
Log in

Optimization and analysis of ultrasound-assisted extraction of bioactive polyphenols from Garcinia indica using RSM and ANFIS modeling and its anticancer activity

  • Original Paper
  • Published:
Journal of the Iranian Chemical Society Aims and scope Submit manuscript

Abstract

Therapeutically important bioactive polyphenols were efficiently extracted from Garcinia indica using ultrasound-assisted extraction (UAE) technique and investigated for its antioxidant and anticancer activities. Response surface methodology-based central composite face-centered experimental design and adaptive neuro-fuzzy inference system were efficiently applied to optimize the effect of UAE parameters for maximum recovery of bioactive polyphenols. The effect of process depending on variables such as ultrasound intensity (X1), methanol concentration (X2), pulse cycle (X3), particle size (X4) and temperature (X5) was studied to obtain highest yields of total phenolic contents (TPC), total flavonoid contents (TFC) and antioxidants. Under optimal condition, the effective combination of variables was observed in the range of X1: 80 W cm−2, X2: 75%, X3: 0.2, X4: 0.1–0.25 mm and X5: 60 °C. Under this optimum condition, the highest yields of TPC and TFC were found to be 698.4–732.76 mg gallic acid equivalent/g and 67.1–72.67 mg rutin equivalent/g of extract. In addition, their antioxidant activities were found to be 62.5–68.31% 1,1-diphenyl-2-picrylhydrazyl scavenging (DPPHsc), 65.26–69.08% 2,2′-azino-bis (ethylbenzothiazoline-6-sulfonic acid scavenging (ABTSsc) and ferric-reducing antioxidant potential value of 68.5–70.12 μg mol (Fe(II))/g. Experimental and predicted values were found to be identical under this optimum circumstance. Further, biological screening of optimally obtained extract displayed a significant anticancer activity against human adenoma breast cancer cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. W. H. Organization, Diet, Nutrition, and the Prevention of Chronic Diseases: Report of a Joint WHO/FAO Expert Consultation, World Health Organization, 2003

  2. M. Arruebo, N. Vilaboa, B. Sáez-Gutierrez, J. Lambea, A. Tres, M. Valladares, Á. González-Fernández, Cancers 3, 3279–3330 (2011)

    Article  CAS  Google Scholar 

  3. J. Mates, Toxicology 153, 83–104 (2000)

    Article  CAS  Google Scholar 

  4. T. Finkel, N.J. Holbrook, Nature 408, 239–247 (2000)

    Article  CAS  Google Scholar 

  5. V.T. Devita Jr., R.C. Young, G.P. Canellos, Cancer 35, 98–110 (1975)

    Article  Google Scholar 

  6. Y. Cai, Q. Luo, M. Sun, H. Corke, Life Sci. 74, 2157–2184 (2004)

    Article  CAS  Google Scholar 

  7. Y. Yukimune, H. Tabata, Y. Higashi, Y. Hara, Nat. Biotechnol. 14, 1129–1132 (1996)

    Article  CAS  Google Scholar 

  8. M. Moudi, R. Go, C.Y.S. Yien, M. Nazre, Int. J. Prev. Med. 4, 1231–1235 (2013)

    PubMed  PubMed Central  Google Scholar 

  9. Z. Su, P. Wang, W. Yuan, G. Grant, S. Li, Nat. Prod. Commun. 12, 1743–1745 (2017)

    Google Scholar 

  10. J. Svasti, C. Srisomsap, P. Subhasitanont, S. Keeratichamroen, D. Chokchaichamnankit, L. Ngiwsara, N. Chimnoi, S. Pisutjaroenpong, S. Techasakul, S.T. Chen, Proteomics 5, 4504–4509 (2005)

    Article  CAS  Google Scholar 

  11. M.-J. Lee, P. Maliakal, L. Chen, X. Meng, F.Y. Bondoc, S. Prabhu, G. Lambert, S. Mohr, C.S. Yang, Cancer Epidemiol. Biomark. Prev. 11, 1025–1032 (2002)

    CAS  Google Scholar 

  12. M. Shoeb, Bangladesh Journal of Pharmacology 1, 35–41 (2006)

    Google Scholar 

  13. T. Winzer, V. Gazda, Z. He, F. Kaminski, M. Kern, T.R. Larson, Y. Li, F. Meade, R. Teodor, F.E. Vaistij, Science 336, 1704–1708 (2012)

    Article  CAS  Google Scholar 

  14. G. Burdock, Food Chem. Toxicol. 36, 347–363 (1998)

    Article  CAS  Google Scholar 

  15. M.S. Baliga, H.P. Bhat, R.J. Pai, R. Boloor, P.L. Palatty, Food Res. Int. 44, 1790–1799 (2011)

    Article  CAS  Google Scholar 

  16. N. Singh. In Vitro Conservation, Ecological Mapping with Chemical and Molecular Characterization of Garcinia Species Occurring in Maharashtra, (2009)

  17. S. Kumar, S. Sharma, S.K. Chattopadhyay, Fitoterapia 89, 86–125 (2013)

    Article  CAS  Google Scholar 

  18. A. Ahmad, S.H. Sarkar, A. Aboukameel, S. Ali, B. Biersack, S. Seibt, Y. Li, B. Bao, D. Kong, S. Banerjee, Carcinogenesis 33, 2450–2456 (2012)

    Article  CAS  Google Scholar 

  19. K. Matsumoto, Y. Akao, E. Kobayashi, T. Ito, K. Ohguchi, T. Tanaka, M. Iinuma, Y. Nozawa, Biol. Pharm. Bull. 26, 569–571 (2003)

    Article  CAS  Google Scholar 

  20. V. Ganesan, V. Gurumani, S. Kunjiappan, T. Panneerselvam, B. Somasundaram, S. Kannan, A. Chowdhury, G. Saravanan, C. Bhattacharjee, J. Food Meas. Character. 12, 228–242 (2018)

    Article  Google Scholar 

  21. K. Vilkhu, R. Mawson, L. Simons, D. Bates, Innov. Food Sci. Emerg. Technol. 9, 161–169 (2008)

    Article  CAS  Google Scholar 

  22. J.P. Maran, S. Manikandan, C.V. Nivetha, R. Dinesh, Arabian J. Chem. 10, S1145–S1157 (2017)

    Article  Google Scholar 

  23. T.J. Mason, L. Paniwnyk, J. Lorimer, Ultrason. Sonochem. 3, S253–S260 (1996)

    Article  CAS  Google Scholar 

  24. M. Pinelo, M. Rubilar, M. Jerez, J. Sineiro, M.J. Núñez, J. Agricul, Food Chem. 53, 2111–2117 (2005)

    Article  CAS  Google Scholar 

  25. N. Foloppe, J. MacKerell, D. Alexander, J. Comput. Chem. 21, 86–104 (2000)

    Article  CAS  Google Scholar 

  26. L.V. Candioti, M.M. De Zan, M.S. Camara, H.C. Goicoechea, Talanta 124, 123–138 (2014)

    Article  Google Scholar 

  27. M.J. Anderson, P.J. Whitcomb, RSM Simplified: Optimizing Processes Using Response Surface Methods for Design of Experiments (Productivity press, Boca Raton, 2016)

    Book  Google Scholar 

  28. K. Selvaraj, R. Chowdhury, C. Bhattacharjee, Int. Food Res. J. 21, 1559–1567 (2014)

    CAS  Google Scholar 

  29. P. Siddhuraju, K. Becker, J. Agricul, Food Chem. 51, 2144–2155 (2003)

    Article  CAS  Google Scholar 

  30. W. Brand-Williams, M.-E. Cuvelier, C. Berset, LWT-Food Sci. Technol. 28, 25–30 (1995)

    Article  CAS  Google Scholar 

  31. I.F. Benzie, J.J. Strain, Anal. Biochem. 239, 70–76 (1996)

    Article  CAS  Google Scholar 

  32. R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, C. Rice-Evans, Free Rad Biol. Med. 26, 1231–1237 (1999)

    Article  CAS  Google Scholar 

  33. J.S. Jang, IEEE Trans. Syst. Man Cybern. 23, 665–685 (1993)

    Article  Google Scholar 

  34. T. Takagi, M. Sugeno, Readings in Fuzzy Sets for Intelligent Systems (Elsevier, Amsterdam, 1993), pp. 387–403

    Book  Google Scholar 

  35. A. Chowdhury, S. Kunjiappan, C. Bhattacharjee, B. Somasundaram, T. Panneerselvam, Vitro Cell. Dev. Biol-Anim. 53, 483–493 (2017)

    Article  CAS  Google Scholar 

  36. H. Le Man, S. Behera, H. Park, Int. J. Environ. Sci. Technol. 7, 157–164 (2010)

    Article  Google Scholar 

Download references

Acknowledgements

The authors are grateful to the Management of Kalasalingam Academy of Research and Education (Deemed to be University), Krishnankoil, Tamilnadu, India, and M.S. Ramaiah University of Applied Sciences, Karnataka, India, for research fellowships and utilizing research facilities. We also thank Prof. Z. Maciej Gliwicz, Ms. Ewa Babkiwics and Dr. Piotr Maszczyk, Department of Hydrobiology, Faculty of Biology, University of Warsaw, Warszawa, Poland, for timely help and support.

Funding

The authors report no financial interest.

Author information

Authors and Affiliations

  1. Department of Biotechnology, Kalasalingam Academy of Research and Education, Krishnankoil, Tamilnadu, 626126, India

    Selvaraj Kunjiappan, Sankarganesh Arunachalam & Suraj Baskararaj

  2. Department of Pharmaceutical Chemistry, Saraswathi College of Pharmacy, Hapur, Uttar Pradesh, 245304, India

    Theivendren Panneerselvam

  3. Department of Pharmaceutical Chemistry, MNR College of Pharmacy, Fasalwadi, Sangareddy, Telangana, 502294, India

    Saravanan Govindaraj

  4. National Cyber Defence Research Centre, Kalasalingam Academy of Research and Education, Krishnankoil, Tamilnadu, 626126, India

    Suthendran Kannan

  5. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, M S Ramaiah University of Applied Sciences, Gnanagangothri Campus, Bengaluru, 560054, India

    Pavadai Parasuraman

  6. Department of Pharmacy, Birla Institute of Technology and Science, Pilani, 333031, India

    Murugesan Sankaranarayanan

  7. School of Mechanical Engineering, Vellore Institute of Technology, Vellore, 632014, India

    Ponnusamy Palanisamy

  8. Department of Pharmacology, Faculty of Pharmacy, M S Ramaiah University of Applied Sciences, Gnanagangothri Campus, Bengaluru, 560054, India

    Damodar Nayak Ammunje

Authors
  1. Selvaraj Kunjiappan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Theivendren Panneerselvam
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Saravanan Govindaraj
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Suthendran Kannan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Pavadai Parasuraman
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Sankarganesh Arunachalam
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Murugesan Sankaranarayanan
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Suraj Baskararaj
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Ponnusamy Palanisamy
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Damodar Nayak Ammunje
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Selvaraj Kunjiappan.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kunjiappan, S., Panneerselvam, T., Govindaraj, S. et al. Optimization and analysis of ultrasound-assisted extraction of bioactive polyphenols from Garcinia indica using RSM and ANFIS modeling and its anticancer activity. J IRAN CHEM SOC 17, 789–801 (2020). https://doi.org/10.1007/s13738-019-01812-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13738-019-01812-1

Keywords

Search

Navigation