Skip to main content
SpringerLink
Log in

Employing peroxidase from Thai indigenous plants for the application of hydrogen peroxide assay

  • Original Paper
  • Published:
Journal of the Iranian Chemical Society Aims and scope Submit manuscript

Abstract

Thai local plants known as banana stalk, banana blossom, banana, sugar-cane, oroxylum indicum fruit, sesbania grandiflora fruit, and pigeon pea fruit were utilized for screening peroxidase enzyme to replace costly horseradish peroxidase in the hydrogen peroxide assay. The highest peroxidase activity was found in banana stalk extracted solution. The kinetic parameters, i.e., Michaelis–Menten constant (Km) and maximum velocity (Vmax) of banana stalk peroxidase were carried out. The optimum pH and thermal stability of this enzyme were also studied. Furthermore, crude banana stalk peroxidase was applied for the determination of hydrogen peroxide in a disinfection solution without any purification. The influent parameters affecting the developed method were cautiously studied and optimized. The calibration curve of standard hydrogen peroxide was achieved between 2.0 and 10.0 μmol L−1 with correlation coefficient (r 2) 0.995. The method validations of detection limit (LOD), limit of quantification (LOQ) and precision were investigated. The concentrations of hydrogen peroxide achieved by the developed method were correlated with the enzymatic method using commercial available horseradish peroxidase.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. D.N. Rani, T.E. Abraham, J. Food Eng. 77, 594 (2006)

    Article  CAS  Google Scholar 

  2. H. Ashraf, Q. Husain, Biotechnol. Bioproc. E. 14, 536 (2009)

    Article  CAS  Google Scholar 

  3. N.D. Srinivas, K.R. Rashmi, K.S.M.S. Raghavarao, Process Biochem. 35, 43 (1999)

    Article  CAS  Google Scholar 

  4. T.H. Gaspar, C.L. Penel, T. Thorpe, H. Grappin, Chemistry and biochemistry of peroxidases, ed. by T.H. Gaspar, C.L. Penel, T. Thorpe, Peroxidases: A survey of their biochemical and physiological roles in higher plants (University de Geneve Press, Geneva, 1982), p. 10

  5. L. Vamos-Vigyazo, Crit. Rev. Food Sci. 15, 49 (1981)

    Article  CAS  Google Scholar 

  6. J.A. Saraiva, C.S. Nunes, M.A. Coimbra, Food Chem. 101, 1571 (2007)

    Article  CAS  Google Scholar 

  7. Y. Kulshrestha, Q. Husain, Biomol. Eng. 23, 291 (2006)

    Article  CAS  Google Scholar 

  8. L. Zhe-fu, C. Li-hong, Z. Wei-qin, Process Biochem. 31, 443 (1996)

    Article  Google Scholar 

  9. A. Ambrosi, M.T. Castañeda, A.J. Killard, M.R. Smyth, S. Alegret, A. Merkoçi, Anal. Chem. 79, 5232 (2007)

    Article  CAS  Google Scholar 

  10. J. Ouyang, J. Delanghe, W.R.G. Baeyens, M. Langlois, Anal. Chim. Acta 362, 113 (1998)

    Article  CAS  Google Scholar 

  11. M. Yang, S. Sun, Y. Kostov, A. Rasooly, Lab Chip 10, 1011 (2010)

    Article  CAS  Google Scholar 

  12. Z. Zhong, W. Wu, D. Wang, D. Wang, J. Shan, Y. Qing, Z. Zhang, Biosens. Bioelectron. 25, 2379 (2010)

    Article  CAS  Google Scholar 

  13. K. Wilberg, C. Assenhaimer, J. Rubio, J. Chem. Technol. Biot. 77, 851 (2002)

    Article  CAS  Google Scholar 

  14. F. Quintanilla-Guerrero, M.A. Duarte-Vázquez, B.E. García-Almendarez, R. Tinoco, R. Vazquez-Duhalt, C. Regalado, Bioresource Technol. 99, 8605 (2008)

    Article  CAS  Google Scholar 

  15. H. Ashraf, Q. Husain, Int. Biodeter. Biodegr. 64, 27 (2010)

    Article  CAS  Google Scholar 

  16. E. Agostini, M.I. Medina, S.R. Milrad De Forchetti, H. Tigier, J. Agr. Food Chem. 45, 596 (1997)

    Article  CAS  Google Scholar 

  17. N.Q. Jia, J. Xu, M.H. Sun, Z.Y. Jiang, Anal. Lett. 38, 1237 (2005)

    Article  CAS  Google Scholar 

  18. Q. Cao, W. Zhou, Anal. Lett. 39, 2725 (2006)

    Article  CAS  Google Scholar 

  19. I. Tiwari, K.P. Singh, M. Singh, B.C. Upadhyay, V.S. Tripathi, Anal. Lett. 43, 2019 (2010)

    Article  CAS  Google Scholar 

  20. I. Da Cruz Vieira, O. Fatibello-Filho, Analyst 123, 1809 (1998)

    Article  Google Scholar 

  21. R.C. Matos, E.O. Coelho, C.F.D. Souza, F.A. Guedes, M.A.C. Matos, Talanta 69, 1208 (2006)

    Article  CAS  Google Scholar 

  22. R.A. de Abreu Franchini, C.F. de Souza, R. Colombara, M.A. Costa Matos, R.C. Matos, J. Agr. Food Chem. 55, 6885 (2007)

    Article  Google Scholar 

  23. B. Habibi, M. Jahanbakhshi, J. Iran. Chem. Soc. 12, 1431 (2015)

    Article  CAS  Google Scholar 

  24. E. Hasheminejad, J.B. Raoof, R. Ojani, S. Rashid-Nadimi, J. Iran. Chem. Soc. 12, 2037 (2015)

    Article  CAS  Google Scholar 

  25. C. Matsubara, K. Kudo, T. Kawashita, K. Takamura, Anal. Chem. 57, 1107 (1985)

    Article  CAS  Google Scholar 

  26. T. Taniai, A. Sakuragawa, T. Okutani, Anal. Sci. 15, 1077 (1999)

    Article  CAS  Google Scholar 

  27. A. Brestovisky, E. KirowaEisner, J. Osteryoung, Anal. Chem. 55, 2063 (1983)

    Article  CAS  Google Scholar 

  28. W. Chen, B. Li, C. Xu, L. Wang, Biosens. Bioelectron. 24, 2534 (2009)

    Article  CAS  Google Scholar 

  29. L. Lu, D. Shiu-Hin Chan, D.W.J. Kwong, H.-Z. He, C.-H. Leung, D.-L. Ma, Chem. Sci. 5, 4561 (2014)

    Article  CAS  Google Scholar 

  30. S. Lin, W. Gao, Z. Tian, C. Yang, L. Lu, J.-L. Mergny, C.-H. Leung, D.-L. Ma, Chem. Sci. 6, 4284 (2015)

    Article  CAS  Google Scholar 

  31. P. Travascio, P.K. Witting, A.G. Mauk, D. Sen, J. Am. Chem. Soc. 123, 1337 (2001)

    Article  CAS  Google Scholar 

  32. T. Niazov, V. Pavlov, Y. Xiao, R. Gill, I. Willner, Nano Lett. 4, 1683 (2004)

    Article  CAS  Google Scholar 

  33. J. Kosman, B. Juskowiak, Anal. Chim. Acta 707, 7 (2011)

    Article  CAS  Google Scholar 

  34. G. Pineiro Avila, M. de la Guardia, Analyst 122, 1543 (1997)

    Article  Google Scholar 

  35. A. Cardinali, L. Sergio, D. Di Venere, V. Linsalata, D. Fortunato, A. Conti, V. Lattanzio, J. Sci. Food Agr. 87, 1417 (2007)

    Article  CAS  Google Scholar 

  36. S.S.M.P. Vidigal, I.V. Tóth, A.O.S.S. Rangel, Microchem. J. 91, 197 (2009)

    Article  CAS  Google Scholar 

  37. J.C. Leon, I.S. Alpeeva, T.A. Chubar, I.Y. Galaev, E. Csoregi, I.Y. Sakharov, Plant Sci. 163, 1011 (2002)

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This research work was kindly supported by Mahasarakham University through the National Research Council of Thailand (NRCT) (Grant No. 5805037/2558). Furthermore, K.P. would like to thank Miss Chanida Sanguansak and Miss Narumool Viengsiri for aiding the experiments. We thank the Center of Excellence for Innovation in Chemistry (PERCH-CIC), Office of the Higher Education Commission, Ministry of Education.

Author information

Authors and Affiliations

  1. Creative Chemistry and Innovation Research Unit, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahasarakham University, Maha Sarakham, 44150, Thailand

    Kraingkrai Ponhong & Watsaka Siriangkhawut

  2. Amnat Charoen Campus, Mahidol University, Amnat Charoen, 37000, Thailand

    Sam-ang Supharoek

  3. Department of Chemistry, Faculty of Science and Center of Excellence for Innovation in Analytical Science and Technology, Chiang Mai University, Chiang Mai, 50200, Thailand

    Kate Grudpan

Authors
  1. Kraingkrai Ponhong
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sam-ang Supharoek
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Watsaka Siriangkhawut
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kate Grudpan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kraingkrai Ponhong.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ponhong, K., Supharoek, Sa., Siriangkhawut, W. et al. Employing peroxidase from Thai indigenous plants for the application of hydrogen peroxide assay. J IRAN CHEM SOC 13, 1307–1313 (2016). https://doi.org/10.1007/s13738-016-0845-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13738-016-0845-7

Keywords

Search

Navigation