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Highly simple and visual colorimetric detection of Brucella melitensis genomic DNA in clinical samples based on gold nanoparticles

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Abstract

Control of Malta fever caused by Brucella melitensis (B. melitensis) in humans depends on the reliability of diagnostic methods used for detection and identification of bacteria. In this study, we described a gold nanoparticles–DNA probes (AuNP–DNA probes) hybridization assay for detecting B. melitensis and its differentiation from other members of Brucella spp. in clinical samples. In this detection method, AuNP–DNA probes were hybridized in a tail-to-tail fashion with BMEII0466 sequence, which is specific to B. melitensis. When hybridization occurred in samples containing B. melitensis genomic DNA, it resulted in the formation of a complex and the solution color shifted from red to purple; therefore, visual detection is possible with the naked eye. The efficacy of AuNP–DNA probe hybridization assay was evaluated from genomic DNA of strains of B. melitensis and non-Brucella spp., and also genomic DNA from clinical samples. The results demonstrated that the main characteristics of this system include easy and rapid operation with high sensitivity and specificity. Therefore, no expensive techniques and equipments are necessary. This method can be employed as a regular diagnostic tool for B. melitensis in clinical samples.

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References

  1. J. Godfroid, A. Cloeckaert, J.P. Liautard, S. Kohler, D. Fretin, K. Walravens, Vet. Res. 36, 313 (2005)

    Article  Google Scholar 

  2. E. Garin-Moreno, I. Moriyon, Proc. Natl. Acad. Sci. USA 99, 1 (2002)

    Article  Google Scholar 

  3. M.G. Kortepeter, G.W. Parker, Emerg. Infect. Dis. 5, 523 (1999)

    Article  CAS  Google Scholar 

  4. G. Pappas, P. Panagopoulou, L. Christou, N. Akritidis, Cell. Mol. Life Sci. 63, 2229 (2006)

    Article  CAS  Google Scholar 

  5. P. Yagupsky, J. Clin. Microbiol. 37, 3437 (1999)

    CAS  Google Scholar 

  6. B.J. Bricker, Vet. Microbiol. 90, 435 (2002)

    Article  CAS  Google Scholar 

  7. E. Navarro, M.A. Casao, J. Solera, Expert Rev. Mol. Diagn. 4, 115 (2004)

    Article  CAS  Google Scholar 

  8. W.F. Klietmann, K.L. Ruoff, Clin. Microbiol. Rev. 14, 364 (2001)

    Article  CAS  Google Scholar 

  9. B.I. Batchelor, R.J. Brindle, G.F. Gilks, J.B. Selkon, J. Hosp. Infect. 22, 159 (1992)

    Article  CAS  Google Scholar 

  10. X. Zhao, R. Tapec-Dytioco, W. Tan, J. Am. Chem. Soc. 125, 11474 (2003)

    Article  CAS  Google Scholar 

  11. E.J. Young, Rev. Infect. Dis. 13, 359 (1991)

    Article  CAS  Google Scholar 

  12. E.J. Young, Clin. Infect. Dis. 21, 283 (1995)

    Article  CAS  Google Scholar 

  13. P.M. Munoz, C.M. Marin, D. Monreal, D. Gonzalez, B. Garin-Bastuji, R. Diaz, R.C. Mainar-Jaime, I. Moriyón, J.M. Blasco1. Clin. Diagn. Lab. Immunol. 12, 141 (2005)

    CAS  Google Scholar 

  14. M.C. Casanas, M.I. Queipo-Ortuno, A. Rodriguez-Torres, A. Orduna, J.D. Colmenero, P. Morata, Eur. J. Clin. Microbiol. Infect. Dis. 20, 127 (2001)

    Article  CAS  Google Scholar 

  15. P. Morata, M.I. Queipo-Ortuno, J.M. Reguera, F. Miralles, J.J. Lopez-Gonzales, J.D. Colmenero, J. Clin. Microbiol. 39, 3743 (2001)

    Article  CAS  Google Scholar 

  16. P. Morata, M.I. Queipo-Ortuno, J.M. Reguera, M.A. Garcia-Ordonez, A. Gardenas, J.D. Colmenero, J. Clin. Microbiol. 41, 144 (2003)

    Article  CAS  Google Scholar 

  17. W.S. Probert, K.N. Schrader, N.Y. Khuong, S.L. Bystrom, M.H. Graves, J. Clin. Microbiol. 42, 1290 (2004)

    Article  CAS  Google Scholar 

  18. M.I. Queipo-Ortuno, J.D. Colmenero, G. Baeza, P. Morata, Clin. Infect. Dis. 40, 260 (2005)

    Article  Google Scholar 

  19. D.S. Leal-Klevezas, I.Q. Martinez-Vazquez, J.P. Martinez-Soriano, J. Clin. Microbiol. 33, 3087 (1995)

    CAS  Google Scholar 

  20. L. Zerva, K. Bourantas, S. Mitka, A. Kansouzidou, N.J. Legakis, J. Clin. Microbiol. 39, 1661 (2001)

    Article  CAS  Google Scholar 

  21. M. Perez-Sancho, T. Garcia-Seco, L. Arrogante, N. Garcia, I. Martinez, A. Diez-Guerrier, A. Perales, J. Goyache, L. Dominguez, J. Alvarez, Res. Vet. Sci. 95, 489 (2013)

    Article  CAS  Google Scholar 

  22. L. Song, J. Li, S. Hou, X. Li, S. Chen, J. Microbiol. Methods 90, 292 (2012)

    Article  CAS  Google Scholar 

  23. P. Bakthavathsalam, V.K. Rajendran, J.A.B. Mohammed, J Nanobiotechnol. 10, 1 (2012)

    Article  Google Scholar 

  24. X. Huang, P.K. Jain, I.H. El-Sayed, M.A. El-Sayed, Nanomedicine (Lond) 2, 681 (2007)

    Article  CAS  Google Scholar 

  25. R. Elghanian, J.J. Storhoff, R.C. Mucic, R.L. Letsinger, C.A. Mirkin, Science 277, 1078 (1997)

    Article  CAS  Google Scholar 

  26. H.D. Hill, C.A. Mirkin, Nat. Protoc. 1, 324 (2006)

    Article  CAS  Google Scholar 

  27. S.F. Altschul, W. Gish, W. Miller, E.W. Myers, D.J. Lipman, J. Mol. Biol. 215, 403 (1990)

    Article  CAS  Google Scholar 

  28. W. Rychlik, Methods Mol. Biol. 402, 35 (2007)

    Article  CAS  Google Scholar 

  29. T. Koressaar, M. Remm, Bioinformatics 23, 1289 (2007)

    Article  CAS  Google Scholar 

  30. K. Nielsen, D. Gall, P. Smith, S. Balsevicius, F. Garrido, M. Ferrer, F. Biancifiori, A. Dajer, E. Luna, L. Samartino, R. Bermudez, F. Moreno, T. Renteria, A. Corral, Rev. Sci. Tech. 23, 979 (2004)

    CAS  Google Scholar 

  31. J.J. Storhoff, S.S. Marla, P. Bao, S. Hagenow, H. Mehta, A. Lucas, Biosens. Bioelectron. 19, 875 (2004)

    Article  CAS  Google Scholar 

  32. J.J. Storhoff, A.D. Lucas, V. Garimella, Y.P. Bao, U.R. Muller, Nat. Biotechnol. 22, 883 (2004)

    Article  CAS  Google Scholar 

  33. J. Godfroid, A. Cloeckaert, J.P. Liautard, S. Kohler, D. Fretin, K. Walravens, B. Garin-Bastuji, J.J. Letesson, Vet. Res. 36, 313 (2005)

    Article  Google Scholar 

  34. C.C. Huang, G.S. Couch, E.F. Pettersen, T.E. Ferrin, Pac Symp. Biocomput. 1, 724 (1996)

    Google Scholar 

  35. V. Hinic, I. Brodard, A. Thomann, M. Holub, R. Miserez, C. Abril, BMC Vet. Res. 5, 1746 (2009)

    Article  Google Scholar 

  36. B. Sumerkan, S. Gokahmetoglu, D. Esel, Clin. Microbiol. Infec. 7, 369 (2001)

    Article  CAS  Google Scholar 

  37. E. Navarro, J. Escribano, J.A. Fernandez, J. Solera, FEMS Immunol. Med. Microbiol. 34, 147 (2002)

    Article  CAS  Google Scholar 

  38. C. Marianelli, A. Martucciello, M. Tarantino, R. Vecchio, G. Iovane, G. Galiero, J. Dairy Sci. 91, 3779 (2008)

    Article  CAS  Google Scholar 

  39. B. Huelseweh, R. Ehricht, H.J. Marschall, Proteomics 6, 2972 (2006)

    Article  CAS  Google Scholar 

  40. X. Zhan, S. Zhou, F. Gong, F. Li, F. Cao, X. Li, J. Cent. South Univ. 41, 890 (2010)

    CAS  Google Scholar 

  41. L.Y. Song, J.T. Li, S.P. Hou, X.D. Li, S.Y. Chen, J. Microbiol. Methods 90, 292 (2012)

    Article  CAS  Google Scholar 

  42. Q. Qu, Z.W. Zhu, Y.F. Wang, Z.J. Zhong, J. Zhao, F. Qiao, X.Y. Du, Z.J. Wang, R.F. Yang, L.Y. Huang, Y.Q. Yu, L. Zhou, Z.L. Chen, J. Microbiol. Methods 79, 121 (2009)

    Article  CAS  Google Scholar 

  43. M. Soleimani, S. Shams, K. Majidzadeh-A, J. Appl. Microbiol. 115, 828 (2013)

    Article  CAS  Google Scholar 

  44. N. Sattarahmady, G.H. Tondro, M. Golchin, H. Heli, Biochem. Eng. J. 97, 1 (2015)

    Article  CAS  Google Scholar 

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Acknowledgments

We would like to thank the Research Councils of Shiraz University of Medical Sciences (8887) and also the Iran National Science Foundation (INSF).

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Authors and Affiliations

  1. Department of Medical Physics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

    Naghmeh Sattarahmady

  2. Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

    Zahra Kayani & Hossein Heli

  3. Department of Nanomedicine, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran

    Hossein Heli

Authors
  1. Naghmeh Sattarahmady
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  2. Zahra Kayani
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  3. Hossein Heli
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Correspondence to Naghmeh Sattarahmady.

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Sattarahmady, N., Kayani, Z. & Heli, H. Highly simple and visual colorimetric detection of Brucella melitensis genomic DNA in clinical samples based on gold nanoparticles. J IRAN CHEM SOC 12, 1569–1576 (2015). https://doi.org/10.1007/s13738-015-0629-5

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  • DOI: https://doi.org/10.1007/s13738-015-0629-5

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