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Solid-phase extraction of l-tryptophan from food samples utilizing a layered double hydroxide nano-sorbent prior to its determination by spectrofluorometry

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Abstract

In this research, a simple method was developed for the separation and determination of l-tryptophan in aqueous solutions. The method consists of a solid-phase extraction (SPE) cleanup system utilizing a column packed with nickel–aluminum layered double hydroxide as a nano-sorbent, followed by a spectrofluorometric detection step. Some factors influencing the SPE efficiency, including pH, the sample flow rate, the elution conditions and the amount of nano-sorbent were evaluated and optimized. The selectivity of the presented SPE method with regard to l-tryptophan was studied in the presence of other amino acids and potentially interfering ions. Under optimum conditions, the calibration graph was linear in the range of 0.1–120.0 µg L−1 with a correlation coefficient of 0.9975. The limit of detection and relative standard deviation for l-tryptophan were 0.01 µg L−1 and 0.62 %, respectively. The maximum sorption capacity of the sorbent for l-tryptophan was 37.6 mg g−1. The method was successfully applied to determine l-tryptophan in several food samples with recoveries in the range of 96.2–104.2 % for the spiked samples.

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References

  1. Q. Jiang, W. Sun, K. Jiao, J. Anal. Chem. 65, 648 (2010)

    Article  CAS  Google Scholar 

  2. X. Zhu, Y. Gu, Anal. Biochem. 401, 260 (2010)

    Article  CAS  Google Scholar 

  3. A.M. Othmana, S. Li, R.M. Leblanc, Anal. Chim. Acta 787, 226 (2013)

    Article  Google Scholar 

  4. Y. Fan, J.H. Liu, H.T. Lu, Q. Zhang, Microchim. Acta 173, 241 (2011)

    Article  CAS  Google Scholar 

  5. F. Jiao, H. Song, W. Yang, X. Jiang, X. Chen, J. Yu, Appl. Clay Sci. 75–76, 92 (2013)

    Article  Google Scholar 

  6. V. Jagannathan, C. March, J. Venitz, Biomed. Chromatogr. 9, 305 (1995)

    Article  CAS  Google Scholar 

  7. H.P. Fitznar, J.M. Lobbes, G. Kattner, J. Chromatogr. A 832, 123 (1999)

    Article  CAS  Google Scholar 

  8. I.A. Arkhipchenko, A.I. Shaposhnikov, L.V. Kravchenko, Appl. Soil Ecol 34, 62 (2006)

    Article  Google Scholar 

  9. S. Hanaoka, J.-M. Lin, M. Yamada, Anal. Chim. Acta 409, 65 (2000)

    Article  CAS  Google Scholar 

  10. W.J.M. Underberg, J.C.M. Waterval, Electrophoresis 23, 3922 (2002)

    Article  CAS  Google Scholar 

  11. F.M. Perrine, B.G. Rolfe, M.F. Hynes, C.H. Hocart, J. Plant Biochem. Biotechnol. 42, 723 (2004)

    CAS  Google Scholar 

  12. L. Michalczuk, J. Chromatogr. 596, 294 (1992)

    Article  CAS  Google Scholar 

  13. G. Ravindran, W.L. Bryden, Food Chem. 89, 309 (2005)

    Article  CAS  Google Scholar 

  14. A. Safavi, S. Momeni, Electroanalysis 22, 2848 (2010)

    Article  CAS  Google Scholar 

  15. A. Özcan, Y. Şahin, Biosens. Bioelectron. 31, 26 (2012)

    Article  Google Scholar 

  16. S. Zhu, J. Zhang, X. Zhao, H. Wang, G. Xu, J. You, Microchim. Acta 181, 445 (2014)

    Article  CAS  Google Scholar 

  17. H. Liu, Y. Chen, Y. Liu, Z. Yang, J. Solid State Electrochem. 17, 2623 (2013)

    Article  CAS  Google Scholar 

  18. A. Żwir-Ferenc, M. Biziuk, Pol. J. Envir. Stud. 15, 677 (2006)

    Google Scholar 

  19. D.G. Evans, R.C.T. Slade, In Layered Double Hydroxides, ed. by X. Duan and D.G. Evans, (Springer, Berlin, 2006) p. 1

  20. P.S. Braterman, Z.P. Xu, F. Yarberry, in Handbook of Layered Materials, ed. by S.M. Auerbach, K.A. Carrado, P.K. Dutta, (Marcel Dekker, Inc., New York, 2004), p. 373

  21. H. Qiu, C. Luo, M. Sun, F. Lu, L. Fan, X. Li, Talanta 98, 226 (2012)

    Article  CAS  Google Scholar 

  22. B. Prasad, M. Tiwari, R. Madhuri, P.S. Sharma, J. Chromatogr. B 879, 364 (2011)

    Article  CAS  Google Scholar 

  23. S. Ghosh, A.Z.M. Badruddoza, M.S. Uddin, K. Hidajat, J. Colloid Interface Sci. 354, 483 (2011)

    Article  CAS  Google Scholar 

  24. H. Abdolmohammad-Zadeh, Z. Rezvani, G.H. Sadeghi, E. Zorufi, Anal. Chim. Acta 685, 212 (2011)

    Article  CAS  Google Scholar 

  25. H. Abdolmohammad-Zadeh, Z. Talleb, Microchim. Acta 179, 25 (2012)

    Article  CAS  Google Scholar 

  26. H. Abdolmohammad-Zadeh, A. Jouyban, R. Amini, G.H. Sadeghi, Microchim. Acta 180, 619 (2013)

    Article  CAS  Google Scholar 

  27. H. Abdolmohammad-Zadeh, S. Kohansal, G.H. Sadeghi, Talanta 84, 368 (2011)

    Article  CAS  Google Scholar 

  28. H. Abdolmohammad-Zadeh, S. Falaghi, E. Rahimpour, Int. J. Cosmetic Sci. 36, 140 (2014)

    Article  CAS  Google Scholar 

  29. H. Abdolmohammad-Zadeh, A. Jouyban, R. Amini, Talanta 116, 604 (2013)

    Article  CAS  Google Scholar 

  30. S. Moore, W.H. Stein, J. Biol. Chem. 211, 907 (1954)

    CAS  Google Scholar 

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Acknowledgments

The financial support from the Research Council of Azarbaijan Shahid Madani University (ASMU, Iran (Grant no. 10799)) is gratefully acknowledged.

Conflict of interest

Hossein Abdolmohammad-Zadeh declares that he has no conflict of interest. Seyed Morteza Hammami Oskooyi declares that he has no conflict of interest.

Human rights

This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. Department of Chemistry and Phytochemistry, Faculty of Sciences, Azarbaijan Shahid Madani University, 35 km Tabriz-Marageh Road, P.O. Box 53714-161, Tabriz, Iran

    Hossein Abdolmohammad-Zadeh & Seyed Morteza Hammami Oskooyi

Authors
  1. Hossein Abdolmohammad-Zadeh
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  2. Seyed Morteza Hammami Oskooyi
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Correspondence to Hossein Abdolmohammad-Zadeh.

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Abdolmohammad-Zadeh, H., Hammami Oskooyi, S.M. Solid-phase extraction of l-tryptophan from food samples utilizing a layered double hydroxide nano-sorbent prior to its determination by spectrofluorometry. J IRAN CHEM SOC 12, 1115–1122 (2015). https://doi.org/10.1007/s13738-014-0572-x

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  • DOI: https://doi.org/10.1007/s13738-014-0572-x

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