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Effects of formulation properties on sol–gel behavior of chitosan/glycerolphosphate hydrogel

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Abstract

Chitosan solution containing glycerolphosphate disodium salt (Gp) is an injectable thermosensitive in situ gel-forming system which undergoes sol–gel transition under certain physiological pH and temperature conditions. When a drug-incorporated chitosan/Gp solution is injected into the body, it forms a three-dimensional gel at 37 °C, which allows the drug to be released in a sustained manner. This hydrogel can be used as a drug delivery system for prolonged release of peptides and glycopeptides. The objective of this work was to investigate the effect of different excipients on the sol–gel behavior of this thermosensitive hydrogel. Chitosan polymeric solutions (2 % w/v) containing Gp and different excipients, such as hydroxypropyl methyl cellulose (HPMC), polyethylene glycol (PEG) with two different molecular weights (PEG200 and PEG1000), and poloxamer (F127) in various concentrations, were prepared, and the pH, sol–gel transition time, and syringeability of the final solutions were evaluated. The obtained results point to HPMC as the best additive for chitosan/Gp solutions in developing an in situ gel-forming drug delivery system with optimum gelling time. A significant decrease was noted in the sol-to-gel transition time (from 90 to 60 s) when HPMC was added to the system. This may have been due to the HPMC structure which acted as a viscosity-enhancing and gel-promoting agent. The in vitro release of vancomycin hydrochloride from chitosan/Gp/HPMC hydrogel was also studied. Vancomycin release studies showed a sustained release profile for over 20 days. It can be concluded that combining chitosan/Gp and HPMC is a promising strategy for preparing a thermally reversible in situ gel-forming delivery system with an optimized gelation time.

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Acknowledgments

This project was supported by a grant from the Vice Chancellor of Research, Mashhad University of Medical Sciences (MUMS), Mashhad, Iran. The results described in this paper were part of a Pharm D student’s thesis proposal related to Ms. M. Sadoogh.

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Authors and Affiliations

  1. Targeted Drug Delivery Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

    Elham Khodaverdi, Mohsen Tafaghodi & Maryam Sadoogh

  2. Department of Pharmaceutics, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

    Elham Khodaverdi, Mohsen Tafaghodi & Maryam Sadoogh

  3. Biomedical Engineering Group, Department of Chemical Engineering, TarbiatModares University, Tehran, Iran

    Fariba Ganji

Authors
  1. Elham Khodaverdi
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  2. Fariba Ganji
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  3. Mohsen Tafaghodi
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  4. Maryam Sadoogh
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Correspondence to Fariba Ganji.

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Khodaverdi, E., Ganji, F., Tafaghodi, M. et al. Effects of formulation properties on sol–gel behavior of chitosan/glycerolphosphate hydrogel. Iran Polym J 22, 785–790 (2013). https://doi.org/10.1007/s13726-013-0177-8

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  • DOI: https://doi.org/10.1007/s13726-013-0177-8

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