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Antimycobacterial, antimicrobial, antioxidant activities and in silico PASS investigations of root fractions and extract of Cordia dichotoma Forst

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Abstract

Indian cherry (Cordia dichotoma Forst.) is a medicinally important plant widely consumed as an indigenous remedy for several ailments in the India. Although it is widely used plant by the population, there are no studies on root proving these medicinal claims. The present study was designed to investigate antimicrobial, antimycobacterial and antioxidant activities of Cordia dichotoma Forst. roots and in silico biological activities prediction using PASS. Methanolic extract was prepared and further fractions were obtained by liquid–liquid partitioning. Agar well diffusion assay and disc diffusion method were selected for antimicrobial activity. Antimycobacterial activity was estimated against Mycobacterium tuberculosis. For antioxidant activity, DPPH and hydrogen peroxide (H2O2) radical scavenging assay were employed. All the tested samples exhibited diverse pattern of sensitivity for tested strains. The value of MIC, MMC and MIC index showed that P. vulgaris and K. pneumonia were the most sensitive for ME and CF, whereas A. fumigates and V. myditis were sensitive to CF and AF. Microorganism susceptible index was 100 for P. vulgaris and K. pneumonia bacteria, and A. fumigates and V. myditis fungi. CF exhibited best activity against M. tuberculosis with lowest MIC of 30 mg/ml and highest activity index of 0.85. Dose dependent antioxidant activity was observed in both the assay. This data provides evidence that Cordia dichotoma Forst. roots have potent antibacterial, antimycobacterial, antioxidant, and moderate antifungal activity and a potential cure for infectious diseases like tuberculosis.

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Acknowledgements

The authors are grateful to Swami Ramanand Teerth Marathwada University, Nanded, Maharashtra, India for financial support (Grant Ref. No. Acctts/Budget/2012-13/2169-2209). Also, the authors are thankful to Director, School of Life Sciences for providing laboratory facilities for performing this research work.

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  1. Centre for Research in Pharmaceutical Sciences, Sharda Bhavan Education Society’s Nanded Pharmacy College, Nanded, Maharashtra, 431606, India

    Prasad Govindrao Jamkhande & Mahavir H. Ghante

  2. School of Pharmacy, Swami Ramanand Teerth Marathwada University, Nanded, Maharashtra, 431606, India

    Sonal Ramrao Barde

  3. Latur college of Pharmacy, Hasegaon, Ausa Latur, Maharashtra, 413512, India

    Balaji R. Ajgunde

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  1. Prasad Govindrao Jamkhande
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Prasad Govindrao Jamkhande has no conflict of interest. Mahavir H. Ghante has no conflict of interest. Sonal Ramrao Barde has no conflict of interest. Balaji R. Ajgunde has no conflict of interest.

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Jamkhande, P.G., Ghante, M.H., Barde, S.R. et al. Antimycobacterial, antimicrobial, antioxidant activities and in silico PASS investigations of root fractions and extract of Cordia dichotoma Forst. Orient Pharm Exp Med 19, 485–496 (2019). https://doi.org/10.1007/s13596-019-00399-5

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