Abstract
The primary objective of this study was to investigate the pharmacological potential of the ethanolic extract of Zizyphus mauritiana bark. Antioxidant, analgesic, cytotoxic and antidiarrheal activities of the extract were investigated using 1, 1-diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging assay, acetic acid induced writhing inhibition, brine shrimp lethality bioassay and castor oil induced diarrhea inhibition respectively. The extract showed remarkable free radical scavenging activity with an IC50 of 27.47 μg/mL. The extract produced significant (P < 0.001) writhing inhibition at the doses of 250 mg and 500 mg/kg in acetic acid induced writhing in mice. With the extract, significant lethality to brine shrimp was found with LC50 value at 92.79 μg/mL. In antidiarrheal activity test, the extract produced 1.25 h latent period of diarrheal induction and 75.2% inhibition in diarrheal episodes compared to positive standard which showed 1.3 h of latent period and 80.55% inhibition in diarrheal episodes. Different phytoconstituents present in the bark might be responsible for biological activities found with this study. The results of this study support the traditional uses of this plant part and could form the basis of further investigation including compound isolation.
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Adzu B, Amos S, Wambebe C, Gamaniel K (2001) Antinociceptive activity of Ziziphus spinachristi root bark extract. Fitoterapia 72:334–350
Ahmed M, Shikha HA, Sadhu SK, Rahman MT, Datta BK (2001) Analgesic, diuretic, and anti-inflammatory principle from Scoparia dulcis. Pharmazie 56(8):657–660
Altman DF (2001) Drugs used in gastrointestinal diseases. In: Katzung BG (ed) Basic and clinical pharmacology, 8th edn. McGraw Hill, San Francisco, pp 1070–1071
Arnous A, Makris DP, Kefalas P (2001) Effect of principal polyphenolic components in relation to antioxidant characteristics of aged red wines. J Agric Food Chem 49:5736–742
Aruoma OI (2003) Methodological considerations for characterizing potential antioxidant actions of bioactive components in food plants. Mutat Res 523–524:9–20
Atta AH, Mouneir SM (2004) Antidiarrheal activity of some Egyptian medicinal plant extracts. J Ethnopharmacol 92:303–09
Bannerman PGC, Mirsky R, Jessen KR, Timpl R, Duance VC (1986) Light microscopic immunolocalization of laminin, type IV collagen, nidogen, heparan sulphate proteoglycan and fibronectin in the enteric nervous system of rat and guinea pig. J Neurocytol 15:432–443
Bhatia A, Mishra T (2010) Hypoglycemic activity of Ziziphus mauritiana aqueous ethanol seed extract in alloxan-induced diabetic mice. Pharm Biol 48(6):604–610
Bhuiyan MAR, Hoque MZ (2010) Free radical scavenging activities of Zizyphus mauritiana. Elec J Envir Agricul Food Chem 9(1):199–206
Birs W, Heller W, Micheal C, Satan M (1991) Flavonoids as antioxidant. Determination of antiradical efficiencies. Methods litcymal 186:343–353
Calixto JB, Beirith A, Ferreira J, Santos AR, Cechinel filho V, Yunes RA (2000) Naturally occurring anti-nociceptive substances from plants. Phytother Res 14:401–418
Carlos CC, Saniel MC (1990) Etiology and epidemiology of diarrhoea. Phillips J Microbio Infect Dis 19:51–53
Chevallier A (1996) The Encyclopedia of medicinal plants, Ist ednth edn. DK Publishing Inc., New York, USA, pp 55–281
Dahiru D, Obidoa O (2008) Evaluation of the antioxidant effects of Ziziphus mauritiana lam. Leaf extracts against chronic ethanolinduced hepatotoxicity in rat liver. Afr J Trad CAM 5(1):39–45
Dahiru D, William ET, Nadro MS (2005) Protective effect of Ziziphus mauritiana leaf extract on carbon tetrachloride-induced liver injury. Afr J Biotechnol 4(10):1177–179
Dahiru D, Sini JM, John-Africa L (2006) Antidiarrhoeal activity of Ziziphus mauritiana root extract in rodents. Afr J Biotechnol 5(10):941–945
Das AK, Mandal SC, Banerjee SK, Sinha S, Das J, Saha BP, Pal M (1999) Studies of antidiarrheal activity of Punica granatum seed extracts. J Ethnopharmacol 68:205–208
Derardt R, Jougney S, Delevalcee F, Falhout M (1980) Release of prostaglandins E and F in an algogenic reaction and its inhibition. Eur J Pharmacol 51:17–24
Devi S, Pandey VB, Singh JP, Shah AH (1987) Peptide alkaloids from Zizyphus species. Phytochem 26(1):3374–3375
Evans WC (1989) Trease and Evan’s textbook of pharmacognosy, 13th edn. Cambridge University Press, London
Farnsworth NR, Soejarto DD (1991) Global importance of medicinal plants. In: Akerele O, Heywood V, Synge H (eds) The conservation of medicinal plants. Cambridge University Press, Cambridge, pp 25–51
Fernanda LB, Victor AK, Amelia TH, Elisabetsky E (2002) Analgesic properties of umbellatine from psychotria umbellate. Pharm Biol 44:54–56
Franzotti EM, Santos CVF, Rodrigues HMSL, Mourao RHV, Andrade MR, Antoniolli AR (2002) Anti-inflammatory, analgesic and acute toxicity of Sida cardiafolia L. J Ethnopharmacol 72:273–278
Gaginella TS, Phillips SF (1975) Ricinoleic acid: current view of ancient oil. Digest Dis Sci 23:1171–1177
Gani A (2003) Medicinal plants of Bangladesh. Chemical Constituents and Uses. Dhaka, Asiatic Society of Bangladesh, p 434
Goodman A, Gillman A (Editors) (1996): The Pharmacological Basis of Therapeutics. (9th Edition), Macmillan Publishers, New York. pp. 924–926.
Gulcin I, Buyukokuroglu ME, Oktay M, Kufrevioglu OI (2002) On the in vitro antioxidant properties of melatonin. J Pineal Res 33:167–171
Gupta M, Mazumdar UK, Sivahkumar T, Vamis MLM, Karki S, Sambathkumar R, Manikandan L (2003) Antioxidant and anti-inflammatory activities of Acalypha fruticosa. Nig J Nat Prod Med 07:25–29
Gupta M, Mazumder UK, Vamsi MLM, Sivakumar T, Kandar CC (2004) Anti steroidogenic activity of the two Indian medicinal plants in mice. J Ethnopharmacol 90(1):21–25
Jadhav HR, Bhutani KK (2002) Antioxidant properties of Indian medicinal plant. Phytother Res 16:771–773
Jarald EE, Joshi SB, Jain DC (2009) Antidiabetic activity of extracts and fraction of zizyphus mauritiana. Pharm Biol 47(4):328–334
Kaaria I (1998) Seed production, dispersal and germination in Cryptostegia grandifolia and Ziziphus mauritiana, two invasive shrubs in tropical woodlands of Northern Australia. Aus J Ecol 21:324–331
Kong JM, Goh NK, Chia LS, Chia TF (2003) Recent advances in traditional plant drugs and orchids. Acta Pharmacol Sin 24:7–21
Lee MF, Chen YH, Lan JL, Tseng CY, Wu CH (2004) Allergenic components of Indian jujube (Zizyphus mauritiana) show IgE cross-reactivity with latex allergen. Int Arch Aller Immunol 133(3):211–16
Lork D (1983) A new approach to acute toxicity testing. Archives Toxicol 54:275–287
Lu F, Foo LY (1995) Toxicological aspects of food antioxidants. In: Madhavi DL, Deshpande SS, Salunkhe DK (eds) Food antioxidants. Marcel Dekker, New York
McLaughlin JL, Anderson JE, Chang CJ (1988) Bioactive components of Allamanda schottii. J Nat Prod 51(2):307–8
McLauglin JL, Chang CJ, Smith DL (1993): Simple bench-top bioassays (brine shrimp and potato discs) for the discovery of plant antitumour compounds. In: Human Medicinal Agents from Plants. Kinghorn, A. D. And Balandrin, M. F. (Eds.), ACS Symposium 534, Am Chem Soc, Washington DC: 112–137.
Meyer BN, Ferrigni NR, Putnam JE, Jawbson LB, Nicholas DE, McLaughlin JL (1982) Brine shrimp: a convenient general bioassay for active plant constituents. Planta Med 45:31–34
Michel A (2002) Tree shrub and Liana of West African zone. Margraf Publishers GMBH, Paris, 440
Nwodo OFC, Alumanah EO (1991) Studies on Abrus Precatorius seeds II: antidiarrheal activity. J Ethnopharmacol 31(3):391–395
Ojewole JAO (2004) Evaluation of antidiarrheal, anti-inflammatory and antidiabetic properties of Sclerocarya birrea (A. Rich.) Hochst. stem bark aqueous extract in mice and rats. Phytotherapy Res 18:601–608
Onasanwo SA, Elegbe RA (2006) Antinociceptive and anti-inflammatory properties of the leaf extracts of Hedranthera barteri in rats and mice. African J Biomed Res 9:117–119
Rahman MAA, Moon SS (2007) Antioxidant polyphenol glycosides from the plant Draba nemorosa. Bull Korean Chem Soc 28(5):827–831
Robaki, Glyglewski RJ (1988) Flavonoids are scavengers of supeoxide anim. Biochem Pharmacol 37:837–841
Sadhu SK, Okuyama E, Fujimoto H, Ishibashi M (2003) Separation of Leucas aspera, a medicinal plant of Bangladesh, guided by prostaglandin inhibitory and antioxidant activities. Chem Pharm Bull 51(5):595–598
Said HM (1996): Medicinal Herbal, Ist edn., Hamdard Foundation Pakistan, p.7–268.
Sawadogo WR, Boly R, Lompo M, Some N (2006) Anti-inflammatory, analgesic and antipyretic activities of Dicliptera verticillata. In J Pharmacol 2:435–438
Schulz V, Hänsel R, Tyler VE (2002) Fitoterapia racional: Um Guia de Fitoterapia para as Ciências da Saúde, 4th edn. Barueri, Manole, p 386
Srivastava SK, Srivastava SD (1979) Structure of zizogenin, a new sapogenin from Zizyphus mauritania. Phytochem 18(10):1758–1759
Taesotikul T, Panthong A, Kanjanapothi D, Verpoorte R, Scheffer JJC (2003) Anti-inflammatory, antipyretic and antinociceptive activities of Tabernaemontana pandacaqui Poir. J Ethnopharmacol 84:31–35
Vyklicky L (1979) Techniques for the study of pain in animals, p.773–778. In: Bonica JJ, Liebeskin, Albe-Fessard DG (eds) Advances in pain research and therapy. Raven, New York, USA
Wadekar RR, Patil KS (2008) Effect of Ziziphus mauritiana leaf extracts on phagocytosis by human neutrophils. J Pharm Res 1(1):79–83
Wagner H (1989) Search for new plant constituents with potential anti-phlogistic and anti- alergic activity. Planta Med 55:235–241
Whittle BA (1964) The use of changes in capillary permeability in mice to distinguish between narcotic and non-narcotic analgesics. British J Pharmacol Chem 22:246–253
Xu GJ (1996) The Chinese materia medica. Volume 1. Chinese Medicine and Technology, Beijing, China
Yusuf M, Chowdhury JU, Wahab MA, Begum J (1994) Medicinal plants of Bangladesh. BCSIR, Dhaka, pp 17–266
Zavala MA, Perez S, Perez C, Vargal R, Perez RM (1998) Antidiarrhoeal activity of Waltheria americana, Commelins cuelestris and Alternanthra repens. J Ethnopharmacol 61:41–47
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The author thank Pharmacy Discipline, Khulna University for necessary funding to conduct this research. The author also thank Department of Botany, Carmichael University College, Rangpur to help identify the plant.
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Rahman, S. Antioxidant, analgesic, cytotoxic and antidiarrheal activities of ethanolic Zizyphus mauritiana bark extract. Orient Pharm Exp Med 12, 67–73 (2012). https://doi.org/10.1007/s13596-011-0042-2
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DOI: https://doi.org/10.1007/s13596-011-0042-2