Abstract
• Context
Handroanthus impetiginosus is an ornamental tree used in urban forestry programs and landscaping, produces good timber, and has medicinal qualities. Clearing of natural forest has been increased and motivates to find techniques that optimize rhizogenesis to ex situ conservation and reforestation.
•Aims
This study evaluated Azospirillum brasilense as an enhancer of in vitro rooting of H. impetiginosus under different indole-3-butyric acid (IBA) pulse inductions and media formulations.
•Methods
In vitro shoots were induced with 0 to 50-μM IBA pulse for 3 days, transferred to two half-strength media (Murashige and Skoog salts with Gamborg’s vitamins [MSG] and woody plant medium), and inoculated or not with A. brasilense Cd or Az39 strains.
•Results
The statistic response surface methodology determined that both A. brasilense strains decreased auxin requirement for rooting up to 49 % in half-strength MSG. In this medium, Cd strain with 30-μM IBA pulse produced the 98 % of rooting shoots 21 days before uninoculated plants induced with the same IBA pulse. Also, the biometric parameter index increased from 127 to 286 % in shoots inoculated and induced with 0 to 30 μM IBA, relative to uninoculated.
•Conclusion
Our results show that biofertilization with A. brasilense significantly promotes in vitro rooting of H. impetiginosus decreasing auxin requirements and micropropagation costs.
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Funding
This research was supported by a grant from the Department of Basic Sciences, National University of Luján, Argentina. Ezequiel Larraburu is a doctoral student in Applied Sciences at the National University of Luján.
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Handling Editor: Douglass Jacobs
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Ezequiel Larraburu and Berta Llorente conceived and designed research and then analyzed the obtained data. Ezequiel Larraburu conducted experiments. Berta Llorente supervised the work and wrote the manuscript. Both authors read and approved the manuscript.
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Larraburu, E.E., Llorente, B.E. Azospirillum brasilense enhances in vitro rhizogenesis of Handroanthus impetiginosus (pink lapacho) in different culture media. Annals of Forest Science 72, 219–229 (2015). https://doi.org/10.1007/s13595-014-0418-9
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DOI: https://doi.org/10.1007/s13595-014-0418-9