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Diversity of ophiostomatoid fungi associated with the large pine weevil, Hylobius abietis, and infested Scots pine seedlings in Poland

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Abstract

Context

Bark beetles are known to be associated with fungi, especially the ophiostomatoid fungi. However, very little is known about role of pine weevils, e.g., Hylobius abietis, as a vector of these fungi in Europe.

Aims

The aims of our study were to demonstrate the effectiveness of H. abietis as a vector of ophiostomatoid fungi in Poland and to identify these fungi in Scots pine seedlings damaged by weevil maturation feeding.

Methods

Insects and damaged Scots pine seedlings were collected from 21 reforestation sites in Poland. The fungi were identified based on morphology, DNA sequence comparisons for two gene regions (ITS, β-tubulin) and phylogenetic analyses.

Results

Sixteen of the ophiostomatoid species were isolated and identified. In all insect populations, Leptographium procerum was the most commonly isolated fungus (84 %). Ophiostoma quercus was also found at a relatively high frequency (16 %). Other ophiostomatoid fungi were found only rarely. Among these rarer fungi, four species, Leptographium lundbergii, Ophiostoma floccosum, Ophiostoma piliferum and Sporothrix inflata, were isolated above 3 %. L. procerum was isolated most frequently and was found in 88 % of the damaged seedlings. S. inflata was isolated from 26 %, while O. quercus occurred in 10 % of the seedlings.

Conclusion

This study confirmed that L. procerum and O. quercus were common associates of H. abietis, while others species were found inconsistently and in low numbers, indicating causal associations. H. abietis also acted as an effective vector transmitting ophiostomatoid species, especially L. procerum and S. inflata, to Scots pine seedlings.

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Funding

The work was supported by grants from the Polish Ministry of Education and Science N N309 049037.

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Authors and Affiliations

  1. Department of Forest Pathology, University of Agriculture in Kraków, Al. 29 Listopada 46, 31-425, Kraków, Poland

    Robert Jankowiak

  2. Department of Forest Protection, Forest Entomology and Climatology, University of Agriculture in Kraków, Al. 29 Listopada 46, 31-425, Kraków, Poland

    Piotr Bilański

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  1. Robert Jankowiak
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Correspondence to Robert Jankowiak.

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Handling Editor: Francois Lieutier

Contribution of the co-authors

Robert Jankowiak: writing the paper, collecting of samples, identification of fungi and analysing the data.

Piotr Bilański: collecting of samples and analysing the data.

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Jankowiak, R., Bilański, P. Diversity of ophiostomatoid fungi associated with the large pine weevil, Hylobius abietis, and infested Scots pine seedlings in Poland. Annals of Forest Science 70, 391–402 (2013). https://doi.org/10.1007/s13595-013-0266-z

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