Skip to main content
SpringerLink
Log in

Lack of genetic differentiation after host range extension argues for the generalist nature of Pityogenes chalcographus (Curculionidae: Scolytinae)

  • Original Paper
  • Published:
Annals of Forest Science Aims and scope Submit manuscript

Abstract

Context

The bark beetle, Pityogenes chalcographus, is one of the main pests in Picea abies stands, and it has also been found on other European Pinaceae species. With massive introductions of North American conifer species into European forests, this insect has extended its host range to exotic Pinaceae species.

Aim

This study assessed whether a wider host range has influenced the genetic structure of P. chalcographus and has led to host specialization.

Methods

Insects were collected from two different regions of France, where eight native and exotic conifer species coexist and were analyzed using mitochondrial and nuclear genetic markers.

Results

Considerable haplotypic diversity was observed within the regions and within host species from where P. chalcographus populations were collected. No genetic differentiation, especially with respect to host species associations, could be detected. Moreover, no relationship could be established between closely related P. chalcographus haplotypes and taxonomically related conifer species.

Conclusion

The capacity of P. chalcographus for host shifting and dispersal may have played a key role in the rapid extension of its host range. These findings are important for pest management in forests and health and phytosanitary measures in the timber trade, especially for risk assessment in mixed coniferous forests including tree species of major economic importance.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Agnoletti M, Anderson S (2000) Forest history: international studies on socioeconomic and forest ecosystem change CAB International. CAB International, Wallingford

    Book  Google Scholar 

  • Arthofer W, Avtzis DN, Riegler M, Stauffer C (2010) Mitochondrial phylogenies in the light of pseudogenes and Wolbachia: re-assessment of a bark beetle dataset. ZooKeys 56:269–280

    Article  PubMed  Google Scholar 

  • Avtzis DN, Arthofer W, Stauffer C (2008a) Sympatric occurrence of diverged mtDNA lineages of Pityogenes chalcographus (Coleoptera, Scolytinae) in Europe. Biol J Linn Soc 94:331–340

    Article  Google Scholar 

  • Avtzis DN, Knizek M, Hellrigl K, Stauffer C (2008b) Polygraphus grandiclava (Coleoptera: Curculionidae) collected from pine and cherry trees: A phylogenetic analysis. Eur J Entomol 105:789–792

    Google Scholar 

  • Becerra JX (1997) Insects on plants: macroevolutionary chemical trends in host use. Science 276:253–256

    Article  PubMed  CAS  Google Scholar 

  • Bertheau C, Sallé A, Roux-Morabito G, Garcia J, Certain G, Lieutier F (2009a) Preference-performance relationship and influence of plant relatedness on host use by Pityogenes chalcographus L. (Coleoptera: Scolytinae). Agric For Entomol 11:89–396

    Article  Google Scholar 

  • Bertheau C, Sallé A, Rossi JP, Bankhead-Dronnet S, Pineau X, Roux-Morabito G, Lieutier F (2009b) Colonisation of native and exotic conifers by indigenous bark beetles (Coleoptera: Scolytinae) in France. For Ecol Manage 258:1619–1628

    Article  Google Scholar 

  • Bogdanowicz SM, Wallner WE, Bell J, O’ Dell TM, Harrison RG (1993) Asian gypsy moths (Lepidoptera, Lymantriidae) in North America—evidence from molecular data. Ann Entomol Soc Am 86:710–715

    CAS  Google Scholar 

  • Campbell JC, Steffen-Campbell JD, Werren JH (1993) Phylogeny of the Nasonia species complex (Hymenoptera: Pteromalidae) inferred from internal transcribed spacer (ITS2) and 28rDNA sequences. Ins Mol Biol 2:225–237

    Article  CAS  Google Scholar 

  • Clement M, Posada D, Crandall KA (2000) TCS: a computer program to estimate gene genealogies. Mol Ecol 9:1657–1659

    Article  PubMed  CAS  Google Scholar 

  • Cognato AI, Harlin AD, Fisher ML (2003) Genetic structure among pinyon pine beetle populations (Scolytinae: Ips confusus). Environ Entomol 32:1262–1270

    Article  Google Scholar 

  • Cognato AI, Sperling FAH (2000) Phylogeny of Ips DeGeer species (Coleoptera: Scolytidae) inferred from mitochondrial cytochrome oxidase I DNA sequence. Mol Phylogenet Evol 14:445–460

    Article  PubMed  CAS  Google Scholar 

  • Crandall KA, Templeton AR (1993) Empirical tests of some predictions from coalescent theory with applications to intraspecific phylogeny reconstruction. Genetics 134:959–969

    PubMed  CAS  Google Scholar 

  • Diegisser T, Tritsch C, Seitz A, Johannesen J (2009) Infestation of a novel host plant by Tephritis conura (Diptera: Tephritidae) in northern Britain: host-range expansion or host shift? Genetica 137:87–97

    Article  PubMed  CAS  Google Scholar 

  • Degomez T, Wagner MR (2001) Arthropod diversity of exotic vs. native Robinia species in northern Arizona. Agric For Entomol 3:19–27

    Article  Google Scholar 

  • Drès M, Mallet J (2002) Host races in plant-feeding insects and their importance in sympatric speciation. Phil Trans Roy Soc Lond B Biol Sci 357:471–492

    Article  Google Scholar 

  • Excoffier L, Laval G, Schneider S (2005) Arlequin (version 3.0): an integrated software package for population genetics data analysis. Evol BioinformOnline 1:47–50

    CAS  Google Scholar 

  • Excoffier L, Smouse PE, Quattro JM (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131:479–491

    PubMed  CAS  Google Scholar 

  • Feder JL, Opp S, Wlazlo B, Reynolds K, Go W, Spisak S (1994) Host fidelity is an effective pre-mating barrier between sympatric races of the apple maggot fly. Proc Natl Acad Sci USA 91:7990–7994

    Article  PubMed  CAS  Google Scholar 

  • Feder JL, Chilcote CA, Bush GL (1988) Genetic differentiation between sympatric host races of Rhagoletis pomonella. Nature 336:61–64

    Article  Google Scholar 

  • Felsenstein J (1988) Phylogenies from molecular sequences: inferences and reliability. Annu Rev Genet 22:521–565

    Article  PubMed  CAS  Google Scholar 

  • Gaete-Eastman C, Figueroa CC, Olivares-Donoso R, Niemeyer HM, Ramírez CC (2004) Diet breadth and its relationship with genetic diversity and differentiation: the case of southern beech aphids (Hemiptera: Aphididae). Bull Entomol Res 94:219–227

    Article  PubMed  CAS  Google Scholar 

  • Gandon S, Ebert D, Olivieri I, Michalakis Y (1998) Differential adaptation in spatially heterogeneous environments and host-parasite coevolution. In: Mopper S, Strauss S (eds) Genetic structure and local adaptation in natural insect populations. Chapman and Hall, London, pp 325–340

    Google Scholar 

  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  • Hare JD (1990) Ecology and management of the Colorado potato beetle. Annu Rev Entomol 35:81–100

    Article  Google Scholar 

  • Hedgren PO, Weslien J, Schroeder LM (2003) Risk for attacks and tree mortality caused by Pityogenes chalcographus L. (Col.: Scolytidae) on young Norway spruce close to cut trees. Scand J For Res 18:39–44

    Google Scholar 

  • Hillis DM, Dixon MT (1991) Ribosomal DNA: molecular evolution and phylogenetic inference. Q Rev Biol 66:411–453

    Article  PubMed  CAS  Google Scholar 

  • Horn A, Stauffer C, Lieutier F, Kerdelhué C (2009) Complex postglacial history of the temperate bark beetle Tomicus piniperda L. (Coleoptera, Scolytinae). Heredity 103:238–247

    Article  PubMed  CAS  Google Scholar 

  • Horn A, Roux-Morabito G, Lieutier F, Kerdelhué C (2006) Phylogeographic structure and past history of the circum-Mediterranean species Tomicus destruens Woll. (Coleoptera: Scolytinae). Mol Ecol 15:1603–1615

    Article  PubMed  CAS  Google Scholar 

  • Jaenike J (1990) Host specialization in phytophagous insects. Annu Rev Ecol Syst 21:243–273

    Article  Google Scholar 

  • Kelley ST, Farrell B, Mitton JB (2000) Effects of specialization on genetic differentiation in sister species of bark beetles. Heredity 84:218–227

    Article  PubMed  Google Scholar 

  • Kerdelhue C, Magnoux E, Lieutier F, Roques A, Rousselet J (2006) Comparative population genetic study of two oligophagous insects associated with the same hosts. Heredity 97:38–45

    Article  PubMed  CAS  Google Scholar 

  • Kerdelhué C, Roux-Morabito G, Forichon J, Chambon JM, Robert A, Lieutier F (2002) Population genetic structure of Tomicus piniperda L. (Curculionidae: Scolytidae) on different pine species and validation of T. destruens (Woll.). Mol Ecol 11:483–494

    Article  PubMed  Google Scholar 

  • Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    Article  PubMed  CAS  Google Scholar 

  • Lopez-Vaamonde C, Charles H, Godfray J, Cook JM (2003) Evolutionary dynamics of host-plant use in a genus of leaf-minig moths. Evolution 57:1804–1821

    PubMed  Google Scholar 

  • McPheron BA, Smith DC, Berlocher SH (1988) Genetic differences between host races of Rhagoletis Pomonella. Nature 336:64–66

    Article  Google Scholar 

  • Mopper S (1996) Adaptive genetic structure in phytophagous insect population. Trends Ecol Evol 11:235–238

    Article  PubMed  CAS  Google Scholar 

  • Nilssen AC (1984) Long-range aerial dispersal of bark beetles and bark weevils (Coleoptera, Scolytidae and Curculionidae) in northern Finland. Ann Entomol Fenn 50:37–42

    Google Scholar 

  • Peterson MA, Denno RF (1998) The influence of dispersal and diet breadth on patterns of genetic isolation by distance in phytophagous insects. Am Nat 152:428–446

    Article  PubMed  CAS  Google Scholar 

  • Petit RJ, El MA, Pons O (1998) Identifying populations for conservation on the basis of genetic markers. Conserv Biol 12:844–855

    Article  Google Scholar 

  • Pfeffer A (1994) Zentral- und westpalaarktische Borken- und Kernkafer (Coleoptera: Scolytidae, Platypodidae). In: Brancucci M, Wittmer M (eds) Entomologica Basiliensia 17. Naturhistorisches Museum Basel, Basel, pp 5–310

    Google Scholar 

  • Pons O, Petit RJ (1996) Measuring and testing genetic differentiation with ordered versus unordered alleles. Genetics 144:1237–1245

    PubMed  CAS  Google Scholar 

  • Posada D, Crandall KA (1998) MODELTEST: testing the model of DNA substitution. Bioinformatics 14:817–818

    Article  PubMed  CAS  Google Scholar 

  • Raymond M, Rousset F (1995) GENEPOP: population genetics software for exact tests and eucumenicism. J Heredity 86:248–249

    Google Scholar 

  • Rychlik W (2007) OLIGO 7 Primer Analysis Software. In: Yuryev A (ed) Methods in molecular biology, vol 402, PCR primer design. Humana, Totowa, pp 35–59

    Google Scholar 

  • Sallé A, Arthoffer W, Lieutier F, Stauffer C, Kerdelhué C (2007) Phylogeography of a species-specific insect: genetic structure of Ips typographus in Europe does not reflect the past fragmentation of its host. Biol J Linn Soc 90:239–246

    Article  Google Scholar 

  • Schoonhoven LM, van Loon JJA, Dicke M (2005) Insect–plant biology. Oxford University Press, New York, p 440

    Google Scholar 

  • Simon C, Frati F, Beckenbach A, Crespi B, Liu H, Flook P (1994) Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Ann Entomol Soc Am 87:651–701

    CAS  Google Scholar 

  • Smouse PE, Long JC, Sokal RR (1986) Multiple regression and correlation extensions of the Mantel Test of matrix correspondence. Syst Zool 35:627–632

    Article  Google Scholar 

  • Stauffer C, Lakatos E, Hewitt GM (1999) Phylogeography and postglacial colonization routes of Ips typographus L. (Coleoptera, Scolytidae). Mol Ecol 8:763–773

    Article  Google Scholar 

  • Strong DR, Lawton JH, Southwood TRE (1984) Insects on plants. Blackwell Scientific, Oxford, p 313

    Google Scholar 

  • Sturgeon KB, Mitton JB (1986) Allozyme and morphological differentiation of mountain pine beetles Dendroctonus ponderosae Hopkins (Coleoptera: Scolytidae) associated with host tree. Evolution 40:290–302

    Article  Google Scholar 

  • Swofford DL (2002) PAUP*: Phylogenetic Analysis Using Parsimony (*and other methods). Sinauer Associates, Sunderland, p 142

    Google Scholar 

  • Thompson JD, Higgins DG, Gibson TJ (1994) Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680

    Article  PubMed  CAS  Google Scholar 

  • Vanbergen AJ, Hodgson DJ, Thurlow M, Hartley SE, Watt AD (2003) Food-plant effects on larval performance do not translate into differences in fitness between populations of Panolis flammea (Lepidoptera: Noctuidae). Bull Entomol Res 93:553–559

    Article  PubMed  CAS  Google Scholar 

  • Via S (1990) Ecological genetics and host adaptation in herbivorous insects: the experimental study of evolution in natural and agricultural systems. Annu Rev Entomol 35:4421–4446

    Article  Google Scholar 

  • Zhang DX, Hewitt GM (1996) Nuclear integrations: challenges for mitochondrial DNA markers. Trends Eco Evol 11:247–251

    Article  CAS  Google Scholar 

  • Zobel BG, Van Wyk G, Stahl P (1987) Growing exotic forests. Wiley, New York, p 508

    Google Scholar 

Download references

Acknowledgments

This work is part of the PhD thesis of C. Bertheau carried out under the direction of F. Lieutier with the participation of G. Roux-Morabito as a co-supervisor. We are grateful to Philippe Massot and Dominique Jacquin (Office National des Forêts) for their help in choosing the experimental plots and obtaining coniferous trees and to Jacques Garcia (INRA Orléans) for his field assistance. We thank Christian Stauffer (Vienna, Boku University) for providing European samples. We also thank Emmanuelle Magnoux (INRA Orléans) for her assistance with the ABI 3100 automatic sequencer and Vincent Lesieur (Orléans University trainee) for his technical participation. We are grateful to Dimitrios Avtzis (Vienna, Boku University) for his fruitful discussions and to Christian Stauffer (Vienna, Boku University) for his valuable remarks on the previous manuscript, as well as the two anonymous reviewers for their very useful and constructive comments.

Funding

The work was supported by grants from the French Ministry for Agriculture, Fisheries and Rural Affairs (General Directorate of Forests and Rural Affairs), from the French Ministry of Research and Education and from the Austrian Science Foundation (FWF) P 21147-B17.

Author information

Author notes

  1. Coralie Bertheau

    Present address: Department of Forest & Soil Sciences, Institute of Forest Entomology, Forest Pathology & Forest Protection, BOKU, University of Natural Resources & Life Sciences, Vienna, Austria

Authors and Affiliations

  1. Laboratoire de Biologie des Ligneux et des Grandes Cultures UPRES EA 1207, rue de Chartres, Université d’Orléans, B.P. 6759, 45067, Orléans cedex 2, France

    Coralie Bertheau, Stéphanie Bankhead-Dronnet, Carine Martin, François Lieutier & Géraldine Roux-Morabito

  2. INRA, Zoologie forestière, Ardon, B.P. 20619, 45166, Olivet, France

    Géraldine Roux-Morabito

  3. Department of Forest & Soil Sciences, Institute of Forest Entomology, Forest Pathology & Forest Protection, BOKU, University of Natural Resources & Life Sciences, Hasenauerstrasse 38, Vienna, 1190, Austria

    Coralie Bertheau

Authors
  1. Coralie Bertheau
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Stéphanie Bankhead-Dronnet
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Carine Martin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. François Lieutier
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Géraldine Roux-Morabito
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Coralie Bertheau.

Additional information

Handling Editor: Erwin Dreyer

Contribution of the co-authors

Coralie Bertheau: was a PhD student in the University of Orléans (France) when the experiments took place. Now she is a post-doctoral research associate at the Institute of Forest Entomology, Forest Pathology and Forest Protection, Boku, University of Natural Resources and Life Sciences in Vienna (Austria). She coordinated the project from its elaboration until the submission of the paper. She designed the experiments, sampled beetles, did technical experiments and phylogenetic data analyses, and wrote the paper.

Stéphanie Bankhead-Dronnet: is Assistant Professor at the University of Orléans. She contributed in phylogenetic data analyses and in writing of the paper.

Carine Martin: is a technical assistant at the University of Orléans and participated to the sampling and the experimental work.

François Lieutier: is a Professor at the University of Orléans. He led this project, supervised the work, helped in beetles sampling and read, corrected, discussed and approved the final version of the paper.

Geraldine Roux-Morabito: is Assistant Professor at the University of Orléans and conducts her research at the INRA Orléans in the units of forest zoology. She led the project, supervised the word and participated in the sampling, in phylogenetic data analyses and in writing of the paper.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Fig. S1

(DOC 48 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bertheau, C., Bankhead-Dronnet, S., Martin, C. et al. Lack of genetic differentiation after host range extension argues for the generalist nature of Pityogenes chalcographus (Curculionidae: Scolytinae). Annals of Forest Science 69, 313–323 (2012). https://doi.org/10.1007/s13595-011-0161-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13595-011-0161-4

Keywords

Search

Navigation