Skip to main content
SpringerLink
Log in

Ovary growth and protein levels in ovary and fat body during adult-wintering period in the red mason bee, Osmia rufa

  • Original article
  • Published:
Apidologie Aims and scope Submit manuscript

Abstract

Winter months are supposed to be a crucial period for the development of the reproductive system, especially ovaries, in Osmia rufa females. During 7 months of the wintering period (from September till March), the length and width of terminal oocytes and protein concentrations in ovary and fat body tissue extracts of red mason bee females, O. rufa, were studied. Our observations indicate statistically significant differences in the size of terminal oocytes for three different periods of over-wintering: September–October, November–January and February–March. Ovary growth was fast in the September–October period; it slowed down in November–January, and then increased again in February–March. Time-dependent changes were noted also in the number of oocytes and protein concentrations in ovary and fat body tissue during all wintering periods. During all winter months the number of oocytes progressively increased, but a statistically significant increase was only noticed for the periods September–October and February–March. Similarly, during the first two months (September–October) and the last three (February–April) we observed major changes in protein concentration in ovary and fat body tissue. Our results indicate that adult diapause in O. rufa varies to some extent in comparison to the other insects, probably because the the ovary is not fully inhibited during wintering, including the diapause period. These results allow a better understanding of the wintering strategies, including the diapause period, and may be useful for improving rearing methods of this economically important species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1.
Figure 2.
Figure 3.
Figure 4.
Figure 5.

Similar content being viewed by others

References

  • Adams, T.S., Filipi, P.A., Yi, S.X. (2002) Effect of age, diapause and juvenile hormone on oogenesis and the amount of vitellogenin and vitellin in the twospotted stink bug, Perillus bioculatus (Heteroptera: pentatomidae). J. Insect Physiol. 48, 477–486

    Article  PubMed  CAS  Google Scholar 

  • Arrese, E.L., Soulages, J.L. (2010) Insect fat body: energy, metabolism, and regulation. Annu. Rev. Entomol. 55, 207–225

    Article  PubMed  CAS  Google Scholar 

  • Bosch, J., Kemp, W.P. (2000) Development and emergence of the orchard pollinator Osmia lignaria (Hymenoptera: Megachilidae). Environ. Entomol. 29, 8–13

    Article  Google Scholar 

  • Bosch, J., Kemp, W.P. (2003) Effect of wintering duration and temperature on survival and emergence time in males of the orchard pollinator Osmia lignaria (Hymenoptera: Megachilidae). Environ. Entomol. 32, 711–716

    Article  Google Scholar 

  • Bosch, J., Kemp, W.P. (2004) Effect of pre-wintering and wintering temperature regimes on weight loss, survival and emergence time in mason bee Osmia cornuta (Hymenoptera: Megachilidae). Apidologie 35, 469–479

    Article  Google Scholar 

  • Bosch, J., Sgolastra, F., Kemp, W.P. (2010) Timing of eclosion affects diapause development, fat body consumption and longevity in Osmia lignaria, a univoltine, adult-wintering solitary bee. J. Insect Physiol. 56, 1949–1957

    Article  PubMed  CAS  Google Scholar 

  • Da Cruz-Landim, C., Patrício, K., Antonialli Jr., W.F. (2006) Cell death and ovarian development in highly eusocial bees (Hymenoptera, Apidae): caste differentiation and worker egg laying. Braz. J. Morphol. Sci. 23, 27–42

    Google Scholar 

  • De Wilde, J., Staal, G.B., De Kort, C.A.D., Baard, G. (1968) Juvenile hormone titre in the haemolymph as a function of photoperiodic treatment in the adult Colorado potato beetle (Leptinotarsa decemlineata). Proc. K. Ned. Akad. Wet. C 71, 321–326

    Google Scholar 

  • Denlinger, D.L. (2002) Regulation of diapause. Annu. Rev. Entomol. 47, 93–122

    Article  PubMed  CAS  Google Scholar 

  • Dortland, J.F. (1978) Synthesis of vitellogenins and diapause proteins by the fat body of Leptinotarsa, as a function of photoperiod. Physiol. Ent. 3, 281–288

    Article  CAS  Google Scholar 

  • Dortland, J.F., de Kort, C.A.D. (1978) Protein synthesis and storage in the aft body of the Colorada potato beetle, Leptinotarsa decemlineata. Insect Biochem. 8, 93–98

    Article  CAS  Google Scholar 

  • Felicioli, A., Kristjanson, K., Lepore, A., Pinzauti, M. (2002) The development of the oocyte in the cocoon imago of Osmia cornuta Latr. (Hymenoptera, Megachilidae): dependence on timing and length of cold treatment. Boll. Lab. Ent. agr. Filippo Silvestri 58, 21–29

    Google Scholar 

  • Giejdasz, K., Wilkaniec, Z. (2002) Individual development of the red mason bee (Osmia rufa L., Megachilidae) under natural and laboratory conditions. J. Apic. Sci 46, 51–57

    Google Scholar 

  • Hahn, D.A., Denlinger, D.L. (2007) Meeting the energetic demands of insect diapause: nutrient storage and utilization. J. Insect Physiol. 53, 760–773

    Article  PubMed  CAS  Google Scholar 

  • Hallmen, M. (1988) Die Besiedelung unterschiedlicher künstlicher Nisthilfen durch Osmia rufa L. (Hymenoptera: Megachilidae). Nachr. Ent. Apollo. 9, 199–212

    Google Scholar 

  • Heming, B.S. (2003) Insect Development and Evolution. Cornell University Press, Ithaca NY

    Google Scholar 

  • Herman, W.S., Tatar, M. (2001) Juvenile hormone regulation of longevity in the migratory monarch butterfly. Proc. Biol. Sci. 22, 2509–2514

    Article  Google Scholar 

  • Hondelmann, P., Poehling, H.M. (2007) Diapause and overwintering of the hoverfly Episyrphus balteatus. Entomol. Exp. Appl. 124, 189–200

    Article  Google Scholar 

  • Kostal, V. (2006) Eco-physiological phases of insect diapause. J. Insect Physiol. 52, 113–127

    Article  PubMed  CAS  Google Scholar 

  • Krafsur, E.S., Moon, R.D., Kim, Y., Rosales, A.L. (1999) Dynamics of diapause recruitment in populations of the face fly, Musca autumnalis. Med. Vet. Entomol. 13, 337–348

    Article  PubMed  CAS  Google Scholar 

  • Kristjansson, K. (1992) Development of Solitary Bees as Crop Pollinators, Proceedings of an EC Workshop. Brussels, Belgium, 91–109

  • Macrae, T.H. (2005) Diapause: diverse states of developmental and metabolic arrest. J. Biol. Res. 3, 3–14

    CAS  Google Scholar 

  • Maeta, Y., Kurihara, M. (1971) Anatomical and histological studies on the oogenesis and oosorption of terminal oocytes within the genus Osmia. Kontyu 39, 138–158

    Google Scholar 

  • Nation, J.L. (2002) Insect Physiology and Biochemistry. CRC Press, Boca Raton

    Google Scholar 

  • Roma, G.C., Bueno, O.C., Camargo-Mathias, M.I. (2010) Morpho-physiological analysis of the insect fat body: a review. Micron 41, 395–401

    Article  PubMed  CAS  Google Scholar 

  • Rust, R., Torchio, P., Trostle, G. (1989) Late embryogenesis and immature development of Osmia rufa cornigera (Rossi) (Hymenoptera: Megachilidae). Apidologie 20, 359–367

    Article  Google Scholar 

  • Sgolastra, F., Bosch, J., Molowny-Horas, R., Maini, S., Kemp, W.P. (2010) Effect of temperature regime on diapause intensity in an adult-wintering Hymenopteran with obligate diapause. J. Insect Physiol. 56, 185–194

    Article  PubMed  CAS  Google Scholar 

  • Smith, P.K., Krohn, R.I., Hermanson, G.T., Mallia, A.K., Gartner, F.H., Provenzano, M.D., Fujimoto, E.K., Goeke, N.M., Olson, B.J., Klenk, D.C. (1985) Measurement of protein using bicinchoninic acid. Anal. Biochem. 150, 76–85

    Article  PubMed  CAS  Google Scholar 

  • Tasei, J.N. (1973) Le comportement de nidification chez Osmia cornuta Latr. Et Osmia rufa L. (Hymenoptera; Megachilidae). Apidologie 4, 195–225

    Article  Google Scholar 

  • Tatar, M., Yin, C.M. (2001) Slow aging during insect reproductive diapause: why butterflies, grasshoppers and flies are like worms. Exp. Gerontol. 36, 723–738

    Article  PubMed  CAS  Google Scholar 

  • Wilkaniec, Z., Giejdasz, K. (2003) Suitability of nesting substrates for cavity-nesting bee Osmia rufa. J. Apic. Res. 42, 29–31

    Google Scholar 

  • Wójtowski, F. (1979) Observations on the biology and utilization potential of the red mason bee Osmia rufa L. (Apoidea, Megachilidae) (in Polish). Rocz. AR Pozn. 111, 203–208

    Google Scholar 

  • Wójtowski, F., Wilkaniec, Z. (1978) Rearing and utilization of solitary bees settled in nest traps (in Polish). Instr. Wdroż. AR Pozn. 1, 1–10

    Google Scholar 

Download references

Acknowledgements

This study was supported by grant no. N N311 298935 from the Ministry of Science and Higher Education. The authors would like to thank T. H. Sparks and P. Skórka for their constructive criticism and linguistic corrections of this manuscript.

Contract grant sponsor: Grant no. N N311 298935 from Ministry of Science and Higher Education, Poland

Author information

Authors and Affiliations

  1. Institute of Zoology, Poznań University of Life Sciences, Wojska Polskiego 71C, 60-625, Poznań, Poland

    Oskar Wasielewski & Karol Giejdasz

  2. Department of Animal Physiology and Biochemistry, Poznań University of Life Sciences, Wojska Polskiego 35, 60-637, Poznań, Poland

    Tatiana Wojciechowicz & Marek Skrzypski

Authors
  1. Oskar Wasielewski
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Karol Giejdasz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tatiana Wojciechowicz
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Marek Skrzypski
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Karol Giejdasz.

Additional information

Développement ovarien et niveaux de protéines dans l’ovaire et le corps adipeux lors de la période d’hivernage de l’adulte chez Osmia rufa

Diapause/ corps adipeux/ abeille maçonne/ ovaire/ hivernage / protéines/ abeille solitaire

Ovarwachstum und Proteingehalte im Ovar und Fettkörper während der imaginalen Überwinterungsphase der roten Mauerbiene, Osmia rufa

Diapause/ Fettkörper/ Osmia rufa / Ovar/ Überwinterung/ Proteine/ solitäre Biene

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wasielewski, O., Giejdasz, K., Wojciechowicz, T. et al. Ovary growth and protein levels in ovary and fat body during adult-wintering period in the red mason bee, Osmia rufa . Apidologie 42, 749–758 (2011). https://doi.org/10.1007/s13592-011-0084-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13592-011-0084-y

Keywords

Search

Navigation