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miR-126a-3p targets HIF-1α and alleviates obstructive sleep apnea syndrome with hypertension

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Abstract

The obstructive sleep apnea syndrome (OSAS) is a common sleep-related breathing disorder and an important cause of refractory hypertension. MicroRNAs (miRNAs) are involved in the development of hypertension, but their role in OSAS with hypertension (OSAS-hypertension) has been little studied. Evidence indicates that miR-126a-3p expression is lower in patients with OSAS-hypertension compared with the patients with OSAS alone. However, its role in the pathogenesis of OSAS-hypertension remains unclear. Therefore, this study aims to investigate the role of miR-126a-3p in OSAS-hypertension and to determine whether HIF-1α is involved in this process. Sprague Dawley rats were exposed to chronic intermittent hypoxia (CIH) for 8 weeks to induce OSAS-hypertension. Rat aortic smooth muscle cells (A7r5) were cultured under hypoxia as an in vitro model. Our results showed that rats exposed to 8 week CIH exhibited decreased miR-126a-3p and increased HIF-1α expression. Furthermore, administration of recombinant adeno-associated virus expressing miR-126a-3p (rAAV-miR-126a) counteracted the CIH-induced systolic blood pressure upregulation, oxidase stress, inflammation, and heart and abdominal aorta vascular remodeling. Moreover, the mechanism was associated with its targeted suppression of HIF-1α. These findings suggest that miR-126a-3p might be a novel potential therapeutic target for the treatment of OSAS-hypertension.

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Acknowledgements

We are grateful to the Department of Respiratory Medicine and all the investigators for their support and hard work during the study.

Funding

This work was supported by the Science and Technology Department of Jiangxi Province (20161BBG70231).

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Authors and Affiliations

  1. Department of Respiratory Medicine, The Second Affiliated Hospital of Nanchang University, No.1 Minde Road, Jiangxi, 330006, China

    Lirong He, Xin Liao, Guofeng Zhu & Jiulong Kuang

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  1. Lirong He
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  2. Xin Liao
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  4. Jiulong Kuang
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Correspondence to Jiulong Kuang.

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He, L., Liao, X., Zhu, G. et al. miR-126a-3p targets HIF-1α and alleviates obstructive sleep apnea syndrome with hypertension. Human Cell 33, 1036–1045 (2020). https://doi.org/10.1007/s13577-020-00404-z

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