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MiR-107 suppresses cell proliferation and tube formation of Ewing sarcoma cells partly by targeting HIF-1β

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Abstract

MicroRNAs serve a crucial role in the regulation of malignant biological behavior of Ewing’s sarcoma (ES). Abnormal expression of miR-107 has been reported in a cohort of cancers, while its exact function in ES remains unclear. Hence, we explored the expression of miR-107 in ES cells and detected its effects on the malignant phenotype of ES cells. Firstly, we perceived the under-expression of miR-107 in human ES cells contrast with the human mesenchymal stem cells. Over-expression of miR-107 restrained cell proliferation and tube formation, arrested cell cycle progression, and facilitated cell apoptosis in SK-ES-1 and RD-ES cell lines. Furthermore, hypoxia inducible factor-1β (HIF-1β) was assumed as a target gene of miR-107. We confirmed the target role of HIF-1β in ES cells. Finally, restoring the expression of HIF-1β could partly abolish miR-107-mediated tumor suppression in ES cells. In conclusion, our results advised that miR-107 suppressed the malignant biological ability of ES cells through targeting HIF-1β.

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References

  1. Thorn D, Mamot C, Krasniqi F, Metternich F, Prestin S. Multimodality treatment in Ewing’s sarcoma family tumors of the maxilla and maxillary sinus: review of the literature. Sarcoma. 2016;2016:3872768. doi:10.1155/2016/3872768.

    Article  PubMed  PubMed Central  Google Scholar 

  2. Rupaimoole R, Calin GA, Lopez-Berestein G, Sood AK. miRNA deregulation in cancer cells and the tumor microenvironment. Cancer Discov. 2016;6(3):235–46. doi:10.1158/2159-8290.CD-15-0893.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Slattery ML, Herrick JS, Mullany LE, Wolff E, Hoffman MD, Pellatt DF, et al. Colorectal tumor molecular phenotype and miRNA: expression profiles and prognosis. Mod Pathol. 2016;29(8):915–27. doi:10.1038/modpathol.2016.73.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Zhang Z, Li Y, Huang L, Xiao Q, Chen X, Zhong J, et al. Let-7a suppresses macrophage infiltrations and malignant phenotype of Ewing sarcoma via STAT3/NF-kappaB positive regulatory circuit. Cancer Lett. 2016;374(2):192–201. doi:10.1016/j.canlet.2016.02.027.

    Article  CAS  PubMed  Google Scholar 

  5. Zhang ZC, Liu JX, Shao ZW, Pu FF, Wang BC, Wu Q, et al. In vitro effect of microRNA-107 targeting Dkk-1 by regulation of Wnt/beta-catenin signaling pathway in osteosarcoma. Medicine. 2017;96(27):e7245. doi:10.1097/md.0000000000007245.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Xu H, Zhang Y, Pena MM, Pirisi L, Creek KE. Six1 promotes colorectal cancer growth and metastasis by stimulating angiogenesis and recruiting tumor-associated macrophages. Carcinogenesis. 2017;. doi:10.1093/carcin/bgw121.

    Google Scholar 

  7. Meng S, Cao J, Wang L, Zhou Q, Li Y, Shen C, et al. MicroRNA 107 partly inhibits endothelial progenitor cells differentiation via HIF-1beta. PLoS ONE. 2012;7(7):e40323. doi:10.1371/journal.pone.0040323.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Tarazona N, Navarro L, Cejalvo JM, Gambardella V, Perez-Fidalgo JA, Sempere A, et al. Primary paraesophageal Ewing’s sarcoma: an uncommon case report and literature review. Onco Targets Ther. 2015;8:1053–9. doi:10.2147/OTT.S80879.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Grassetti L, Torresetti M, Brancorsini D, Rubini C, Lazzeri D, Di Benedetto G. A peculiar case of large primary cutaneous Ewing’s sarcoma of the foot: case report and review of the literature. Int J Surg Case Rep. 2015;15:89–92. doi:10.1016/j.ijscr.2015.08.024.

    Article  PubMed  PubMed Central  Google Scholar 

  10. Ren G, Yu B. Post-transcriptional control of miRNA abundance in Arabidopsis. Plant signal Behav. 2012;7(11):1443–6. doi:10.4161/psb.21956.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Sen CK, Ghatak S. miRNA control of tissue repair and regeneration. Am J Pathol. 2015;185(10):2629–40. doi:10.1016/j.ajpath.2015.04.001.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Osaki M, Okada F, Ochiya T. miRNA therapy targeting cancer stem cells: a new paradigm for cancer treatment and prevention of tumor recurrence. Therap Deliv. 2015;6(3):323–37. doi:10.4155/tde.14.122.

    Article  CAS  Google Scholar 

  13. Nugent M. microRNA and bone cancer. Adv Exp Med Biol. 2015;889:201–30. doi:10.1007/978-3-319-23730-5_11.

    Article  CAS  PubMed  Google Scholar 

  14. Iida K, Fukushi J, Matsumoto Y, Oda Y, Takahashi Y, Fujiwara T, et al. miR-125b develops chemoresistance in Ewing sarcoma/primitive neuroectodermal tumor. Cancer Cell Int. 2013;13(1):21. doi:10.1186/1475-2867-13-21.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Cui J, Mo J, Luo M, Yu Q, Zhou S, Li T, et al. c-Myc-activated long non-coding RNA H19 downregulates miR-107 and promotes cell cycle progression of non-small cell lung cancer. Int J Clin Exp Pathol. 2015;8(10):12400–9.

    PubMed  PubMed Central  Google Scholar 

  16. Zhang Z, Zhang L, Yin ZY, Fan XL, Hu B, Wang LQ, et al. miR-107 regulates cisplatin chemosensitivity of A549 non small cell lung cancer cell line by targeting cyclin dependent kinase 8. Int J Clin Exp Pathol. 2014;7(10):7236–41.

    CAS  PubMed  PubMed Central  Google Scholar 

  17. Ayremlou N, Mozdarani H, Mowla SJ, Delavari A. Increased levels of serum and tissue miR-107 in human gastric cancer: correlation with tumor hypoxia. Cancer Biomark. 2015;15(6):851–60. doi:10.3233/CBM-150529.

    Article  CAS  PubMed  Google Scholar 

  18. Wang S, Ma G, Zhu H, Lv C, Chu H, Tong N, et al. miR-107 regulates tumor progression by targeting NF1 in gastric cancer. Sci Rep. 2016;6:36531. doi:10.1038/srep36531.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Villanueva MT. Angiogenesis: a sudden rush of blood to the tumour. Nat Rev Cancer. 2015;15(3):135. doi:10.1038/nrc3914.

    Article  CAS  PubMed  Google Scholar 

  20. van der Schaft DW, Hillen F, Pauwels P, Kirschmann DA, Castermans K, Egbrink MG, et al. Tumor cell plasticity in Ewing sarcoma, an alternative circulatory system stimulated by hypoxia. Can Res. 2005;65(24):11520–8. doi:10.1158/0008-5472.can-05-2468.

    Article  Google Scholar 

  21. Li Y, Mao L, Gao Y, Baral S, Zhou Y, Hu B. MicroRNA-107 contributes to post-stroke angiogenesis by targeting Dicer-1. Sci Rep. 2015;5:13316. doi:10.1038/srep13316.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Deng B, Du J, Hu R, Wang AP, Wu WH, Hu CP, et al. MicroRNA-103/107 is involved in hypoxia-induced proliferation of pulmonary arterial smooth muscle cells by targeting HIF-1beta. Life Sci. 2016;147:117–24. doi:10.1016/j.lfs.2016.01.043.

    Article  CAS  PubMed  Google Scholar 

  23. Lauzier MC, Michaud MD, Dery MA, Richard DE. HIF-1 activation during tumor progression: implications and consequences. Bull Cancer. 2006;93(4):349–56.

    CAS  PubMed  Google Scholar 

  24. Meijer TW, Kaanders JH, Span PN, Bussink J. Targeting hypoxia, HIF-1, and tumor glucose metabolism to improve radiotherapy efficacy. Clin Cancer Res. 2012;18(20):5585–94. doi:10.1158/1078-0432.CCR-12-0858.

    Article  CAS  PubMed  Google Scholar 

  25. Yamakuchi M, Lotterman CD, Bao C, Hruban RH, Karim B, Mendell JT, et al. P53-induced microRNA-107 inhibits HIF-1 and tumor angiogenesis. Proc Natl Acad Sci USA. 2010;107(14):6334–9. doi:10.1073/pnas.0911082107.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

This project is Funded by the National Nature Science Foundation of China (Grant No. 81502329); Program of Science and Technology of ChongQing Commission (Grant No. KJ1600228); Programs of Yongchuan Hospital of ChongQing Medical University (Grant Nos. YJZQN 201514; YCZQN201511).

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Authors and Affiliations

  1. Department of Orthopedics, Yongchuan Hospital, Chongqing Medical University, Chongqing, 402160, People’s Republic of China

    Jiajun Chen, Xin Zhou, Tengyu Wang, Gaohai Shao & Zhongzu Zhang

  2. Department of Gynecology and Obstetrics, Yongchuan Hospital, Chongqing Medical University, Chongqing, 402160, People’s Republic of China

    Yunyun Li

  3. Department of Orthopedics, The First Affiliated Hospital, Nanchang University, Nanchang, 330006, Jiangxi, People’s Republic of China

    Qianren Xiao

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  1. Jiajun Chen
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  2. Xin Zhou
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  3. Qianren Xiao
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  4. Tengyu Wang
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Correspondence to Yunyun Li or Zhongzu Zhang.

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The authors declare that they have no potential conflicts of interest.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the Ethics Committee of Chongqing Medical University and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Informed consent was obtained from all individual participants included in the study.

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Chen, J., Zhou, X., Xiao, Q. et al. MiR-107 suppresses cell proliferation and tube formation of Ewing sarcoma cells partly by targeting HIF-1β. Human Cell 31, 42–49 (2018). https://doi.org/10.1007/s13577-017-0183-9

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  • DOI: https://doi.org/10.1007/s13577-017-0183-9

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