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Cardiomyocyte marker expression in a human lymphocyte cell line using mouse cardiomyocyte extract

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Abstract

Cell transplantation shows potential for the treatment of cardiac diseases. Embryonic stem cells, cord blood and mesenchymal stem cells have been suggested as sources for transplantation therapy. Because of some technical limitations with the use of stem cells, transdifferentiation of fully differentiated cells is a potentially useful alternative. We investigated whether human peripheral blood cells could transdifferentiate into cardiomyocyte. Transdifferentiation was induced in a human B lymphocyte cell line (Raji). Cardiomyocyte extract was prepared from adult mouse cardiomyocytes. The cells were treated with 5-aza-2-deoxycytidine and trichostatin A, permeabilized with streptolysin O, and exposed to the mouse cardiomyocyte extract. They were cultured for 10 days, 3 weeks and 4 weeks. Cardiomyocyte markers were detected with immunohistochemistry and flow cytometry. Immunocytochemistry revealed that some cells expressed myosin heavy chain, α-actinin and cardiac troponin T after 3 and 4 weeks. Flow cytometry confirmed these data. In cells exposed to trichostatin A and 5-aza-2-deoxycytidine and permeabilized in the presence of the cardiomyocyte extract, troponin T expression was seen in 3.53% of the cells and 3.11% of them expressed α-actinin. After exposure to the cardiomyocyte extract, some permeabilized cells adhered to the plate loosely; however, the morphology did not change significantly, and they continued to show a rounded shape after 4 weeks. Our treated lymphocytes expressed cardiomyocyte markers. Our results suggest that lymphocytes may be useful in future research as a source of cells for reprogramming procedures.

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Acknowledgments

The authors thank the Vice-Chancellor for research of the Shiraz University of Medical Sciences for support through grant no. 88-4578, and Ms L. Rohani for excellent technical support. We also thank K. Shashok (Author AID in the Eastern Mediterranean) for improving the use of English in the manuscript, and M. Gholami at the Center for Development of Clinical Research of Nemazee Hospital for research assistance. This study formed part of the work done by S. Tavakolinejad for a thesis.

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Authors and Affiliations

  1. Laboratory for Stem Cell Research, Anatomy Department, Shiraz University of Medical Sciences (SUMS), Shiraz, Iran

    Zahra Vojdani, Sima Tavakolinejad, Tahereh Talaei-Khozani & Tahereh Esmaeilpour

  2. Microbiology Research Center, SUMS, Shiraz, Iran

    Manuchehr Rasooli

  3. School of Medicine, Zand Street, Shiraz University of Medical Sciences, Shiraz, Iran

    Zahra Vojdani

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  1. Zahra Vojdani
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Correspondence to Zahra Vojdani.

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Vojdani, Z., Tavakolinejad, S., Talaei-Khozani, T. et al. Cardiomyocyte marker expression in a human lymphocyte cell line using mouse cardiomyocyte extract. Human Cell 24, 35–42 (2011). https://doi.org/10.1007/s13577-011-0009-0

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  • DOI: https://doi.org/10.1007/s13577-011-0009-0

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