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GC-MS-FID characterization and antibacterial activity of the essential oil from Achyrocline satureioides (Lam) DC

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Abstract

Achyrocline satureioides (Lam.) DC is a plant in the Asteraceae family that is commonly known as macela or marcela. It has antidiarrheal, antispasmodic, analgesic, anti-inflammatory and antimicrobial properties. The species Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli are of great clinical importance, thus more evidence on the discovery of new compounds from plant species is required. The aim of this study was to evaluate the phytochemical profile and in vitro antimicrobial potential of essential oils from A. satureioides. A Minimal Inhibitory Concentration (MIC) test was performed using microdilution, and the chemical composition of the essential oil was analyzed using Gas Chromatography coupled to Mass spectrometry (GC / MS). Using the GC / MS analysis, we identified alpha-pinene and (E)-cariophilene as major phytoconstituents. We observed synergisms when the essential oil was combined with gentamicin and ampicillin against the S. aureus strain, as well as against E. coli when combined with gentamicin and norfloxacin. The results were promising, but further research is needed to assess the toxicity of A. satureioides oil and its potential for therapeutic use in humans.

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Abbreviations

GC–MS:

Gas Chromatography coupled to Mass spectrometry

MIC:

Minimal Inhibitory Concentration

WHO:

World Health Organization

HFIE:

Herbarium of the Integrated Spiritist Faculties

ATCC:

American Type Culture Collection

HIA:

Heart Infusion Agar; LMBM: Laboratório de Microbiologia e Biologia Molecular

URCA:

Universidade Regional do Cariri

CFU:

Colony Forming Unity

BHI:

Brain Heart Infusion

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Authors and Affiliations

  1. Regional University of Cariri - URCA, Rua Cel. Antonio Luis, 1161, Pimenta, Crato, CE, 63105-000, Brazil

    Cícera Natalia Figueiredo Leite Gondim, Joara Nályda Pereira Carneiro, Catarina Pereira Leite & Henrique Douglas Melo Coutinho

  2. Faculty of Medicine, Estácio of Juazeiro Do Norte – Estácio | FMJ, Tenente Raimundo Rocha Avenue, 515, Cidade Universitária, Juazeiro Do Norte, CE, 63040-360, Brazil

    Nadghia Figueiredo Leite Sampaio

  3. Federal University of Cariri - UFCA, Olegário Emidio de Araujo Street, - Centro, Brejo Santo, CE, 63260-000, Brazil

    Jacqueline Cosmo Andrade Pinheiro

  4. Federal University of Paraná - UFPR, XV de Novembro, 1299 Centro, Curitiba, PR, 80,060-000, Brazil

    Wanderlei do Amaral, Cícero Deschamps & Luiz Everson da Silva

  5. University Center Doutor Leão Sampaio – UNILEÃO, Av. Maria Letícia Leite Pereira S/N - Lagoa Seca, Juazeiro Do Norte, CE, 63040-405, Brazil

    Gonçalo Emanuel Carvalho Gondim

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  1. Cícera Natalia Figueiredo Leite Gondim
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  2. Joara Nályda Pereira Carneiro
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  3. Catarina Pereira Leite
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  10. Henrique Douglas Melo Coutinho
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Correspondence to Henrique Douglas Melo Coutinho.

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Gondim, C.N.F.L., Carneiro, J.N.P., Leite, C.P. et al. GC-MS-FID characterization and antibacterial activity of the essential oil from Achyrocline satureioides (Lam) DC. J. Plant Biochem. Biotechnol. 31, 394–398 (2022). https://doi.org/10.1007/s13562-021-00691-z

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