Skip to main content
SpringerLink
Log in

Enhancement of Celastrol compound by silver nanoparticles and acetosyringone in Celastrus paniculatus Willd. through adventitious and hairy root culture

  • Short Communication
  • Published:
Journal of Plant Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Celastrus paniculatus Willd., an important medicinal plant for the treatment of neurodegenerative diseases is at risk of extinction due to destructive harvesting by pharmaceutical industries in its different habitats. This study aims to determine the impact of silver nanoparticles on the enhancement of therapeutic Celastrol content in adventitious and hairy root culture. MS medium supplemented with 0.3 mg L−1 Indole Acetic Acidwas found suitable for adventitious root induction (69%). Hairy roots were successfully induced by co-cultivation of leaf explants with Agrobacterium rhizogenes strain MTCC532 supplemented with acetosyringone (100 µM) to the half-strength medium. Transformation confirmed by PCR analysis.10 µg mL−1 of AgNPs with 48 h exposure time resulted in enhanced biomass accumulation in adventitious roots and in hairy roots. HPLC analysis confirmed that Celastrol content increased 2.24-fold and 1.87-fold higher in hairy root and adventitious roots respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Abbreviations

AgNPs:

Silver nanoparticles

HR:

Hairy root

AR:

Adventitious root

LB:

Luria Bertani Broth

SM:

Secondary metabolites

ANOVA:

Analysis of variance

SEM:

Scanning electron microscope

IBA:

Indole-3-butyric acid

IAA:

Indole acetic acid

HPLC:

High-pressure liquid chromatography

MTCC:

Microbial type culture collection

References

  • Al-Huqail AA, Hatata MM, Al-Huqail AA, Ibrahim MM (2018) Preparation, characterization of silver phyto nanoparticles and their impact on growth potential of Lupinus termis L. seedlings. Saudi J Biol Sci 25:313–319

    Article  CAS  PubMed  Google Scholar 

  • Anjum S, Anjum I, Hano C, Kousar S (2019) Advances in nanomaterials as novel elicitors of pharmacologically active plant specialized metabolites: current status and future outlooks. RSC Adv 9:40404–40423

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Brijwal L, Tamta S (2015) Agrobacterium rhizogenes mediated hairy root induction in endangered Berberis aristata DC. Springer Plus 4:443

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Choi SM, Son SH, Yun SR, Kwon OW, SeonJH PKY (2000) Pilot-scale culture of adventitious roots of ginseng in a bioreactor system. Plant Cell Tissue Org 62:187–193

    Article  CAS  Google Scholar 

  • Doma M, Abhayankar G, Reddy VD, Kishor PB (2012) Carbohydrate and elicitor enhanced withanolide (withaferin A and withanolide A) accumulation in hairy root cultures of Withaniasomnifera (L.). Indian J Exp Biol 50:484–490

    PubMed  Google Scholar 

  • Ercan AG, Taskin KM, Turgut K, Yuce S (1999) Agrobacterium rhizogenes-mediated hairy root formation in some Rubia tinctorum L. populations grown in Turkey. Turk J Bot 23:373–378

    Google Scholar 

  • FaizalA SAV (2019) Enhancement of saponin accumulation in adventitious root culture of Javanese ginseng (Talinum paniculatum Gaertn.) through methyl jasmonate and salicylic acid elicitation. Afr J Biotechnol 18:130–135

    Article  Google Scholar 

  • Gelvin SB (2009) Agrobacterium in the genomics age. Plant Physiol 150:1665–1676

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ghimire BK, Thiruvengadam M, Chung IM (2019) Identification of elicitors enhances the polyphenolic compounds and pharmacological potential in hairy root cultures of Aster scaber. S Afr J Bot 125:92–101

    Article  CAS  Google Scholar 

  • Karthikeyan A, Palanivel S, Parvathy S, Raj RB (2007) Hairy root induction from hypocotyl segments of groundnut (Arachis hypogaea L). Afr J Biotechnol 6:15

    Google Scholar 

  • Kim DH, Shin EK, Kim YH, Lee BW, Jun JG, Park JHY, Kim JK (2009) Suppression of inflammatory responses by celastrol, a quinone methide triterpenoid isolated from Celastrus regelii. Eur J Clin Invest 39:819–827

    Article  CAS  PubMed  Google Scholar 

  • Lal D, Singh N (2010) Mass multiplication of Celastrus paniculatus Willd: an important medicinal plant under in vitro conditions via nodal segments. Int J Biodivers Conserv 2:140–145

    Google Scholar 

  • Lee WM, Kwak JI, An YJ (2012) Effect of silver nanoparticles in crop plants Phaseolus radiatus and Sorghum bicolor: media effect on phytotoxicity. Chemosphere 86:491–499

    Article  CAS  PubMed  Google Scholar 

  • Lynch J (1995) Root architecture and plant productivity. Plant Physiol 109:7

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Malik J, Karan M, Dogra R (2017) Ameliorating effect of Celastrus paniculatus standardized extract and its fractions on 3-nitropropionic acid induced neuronal damage in rats: possible antioxidant mechanism. Pharm Biol 55:980–990

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Manickavasagam M, Subramanyam K, Ishwarya R, Elayaraja D, Ganapathi A (2015) Assessment of factors influencing the tissue culture-independent Agrobacterium-mediated in planta genetic transformation of okra [Abelmoschus esculentus (L.) Moench]. Plant Cell Tissue Org 123:309–320

    Article  CAS  Google Scholar 

  • Marslin G, Sheeba CJ, Franklin G (2017) Nanoparticles alter secondary metabolism in plants via ROS burst. Front Plant Sci 8:832

    Article  PubMed  PubMed Central  Google Scholar 

  • Mayavan S, Subramanyam K, Arun M, Rajesh M, Dev GK, Sivanandhan G, Jaganath B, Manickavasagam M, Selvaraj N, Ganapathi A (2013) Agrobacterium tumefaciens-mediated in planta seed transformation strategy in sugarcane. Plant Cell Rep 32:1557–1574

    Article  CAS  PubMed  Google Scholar 

  • Moola AK, Diana RKB (2019) Elicitation as a means for enhanced plant secondary metabolites through hairy root system. J Adv Sci Res 10:3

    Google Scholar 

  • Moola AK, Kumari BR (2020) Rapid propagation of Celastrus paniculatus Willd.: an endangered medicinal plant through indirect organogenesis. Vegetos 33:277–285

    Article  Google Scholar 

  • Moola AK, Balasubramani S, Nithya C, Diana RKB (2019) Preparation, characterization of silver-nanoparticles from seed coat exudates of Celastrus paniculatus Willd. and their bactericidal and biofilm inhibition effects. Int J Pharm Sci Drug Res 11:164–173

    CAS  Google Scholar 

  • Nandagopal S, Kumari BR (2007) Phytochemical and antibacterial studies of Chicory (Cichorium intybus L.)—a multipurpose medicinal plant. J Adv Sci Res 1:17–21

    Google Scholar 

  • Pitta-Alvarez SI, Spollansky TC, Giulietti AM (2000) The influence of different biotic and abiotic elicitors on the production and profile of tropane alkaloids in hairy root cultures of Brugmansia candida. Enzyme Microb Technol 26:252–258

    Article  CAS  PubMed  Google Scholar 

  • Rahnama H, Razi Z, Dadgar MN, Hasanloo T (2013) Enhanced production of flavonolignans in hairy root cultures of Silybum marianum by over-expression of chalcone synthase gene. J Plant Biochem Biotechnol 22:138–143

    Article  CAS  Google Scholar 

  • Rajasekharan PE, Ganeshan S (2002) Conservation of medicinal plant biodiversity—an Indian perspective. J Trop Med Plants 3:125–140

    Google Scholar 

  • Rajesh M, Sivanandhan G, Arun M, Vasudevan V, Theboral J, Girija S, Manickavasagam M, Selvaraj N, Ganapathi A (2014) Factors influencing podophyllotoxin production in adventitious root culture of Podophyllum hexandrum Royle. Acta Physiol Plant 36:1009–1021

    Article  CAS  Google Scholar 

  • Raju NL, Prasad MNV (2010) Influence of growth hormones on adventitious root formation in semi-hardwood cuttings of Celasturs paniculatus Willd.: a contribution for rapid multiplication and conservation management. Agrofor Syst 79:249–252

    Article  Google Scholar 

  • Saeed S, Ali H, Khan T, Kayani W, Khan MA (2017) Impacts of methyl jasmonate and phenyl acetic acid on biomass accumulation and antioxidant potential in adventitious roots of Ajuga bracteosa Wall ex Benth., a high valued endangered medicinal plant. Physiol Mol Biol Plant 23:229–237

    Article  CAS  Google Scholar 

  • Sathish S, Vasudevan V, Karthik S, Pavan G, Manickavasagam M (2019) Enhanced l-Dopa production from elicited cell suspension culture of Hybanthus enneaspermus (L.) F. Muell. Plant Biotechnol Rep 13:613–621

    Article  Google Scholar 

  • Sharma AK, Prasad RN, Chaturvedi HC (1981) Clonal propagation of Bougainvillea glabra’ Magnifica’ through shoot apex culture. Plant Cell Tiss Org 1:33–38

    Article  Google Scholar 

  • Silja PK, Satheeshkumar K (2015) Establishment of adventitious root cultures from leaf explants of Plumbago rosea and enhanced plumbagin production through elicitation. Ind Crops Prod 76:479–486

    Article  CAS  Google Scholar 

  • Singh S, Pandey P, Ghosh S, Banerjee S (2018) Anti-cancer labdane diterpenoids from adventitious roots of Andrographis paniculata: augmentation of production prospect endowed with pathway gene expression. Protoplasma 255:1387–1400

    Article  CAS  PubMed  Google Scholar 

  • Sivanandhan G, Arun M, Mayavan S, Rajesh M, Jeyaraj M, Dev GK, Manickavasagam M, Selvaraj N, Ganapathi A (2012) Optimization of elicitation conditions with methyl jasmonate and salicylic acid to improve the productivity of withanolides in the adventitious root culture of Withania somnifera (L.) Dunal. Appl Biochem Biotechnol 168:681–696

    Article  CAS  PubMed  Google Scholar 

  • Sivanandhan G, Dev GK, Theboral J, Selvaraj N, Ganapathi A, Manickavasagam M (2015) Sonication, vacuum infiltration and thiol compounds enhance the Agrobacterium-mediated transformation frequency of Withania somnifera (L.) Dunal. PLoS One 10(4):e0124693

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Sreeranjini S, Siril EA (2015) Optimizing elicitors and precursors to enhance alizarin and purpurin production in adventitious roots of Morinda citrifolia L. Proc Natl Acad Sci India Sect B Biol Sci 85:725–731

    Article  CAS  Google Scholar 

  • Srivastava S, Srivastava AK (2007) Hairy root culture for mass-production of high-value secondary metabolites. Crit Rev Biotechnol 27:29–43

    Article  CAS  PubMed  Google Scholar 

  • Srivastava M, Singh G, Sharma S, Shukla S, Misra P (2019) Elicitation enhanced the yield of glycyrrhizin and antioxidant activities in hairy root cultures of Glycyrrhiza glabra L. J Plant Growth Regul 38:373–384

    Article  CAS  PubMed  Google Scholar 

  • Sujatha G, Zdravković-Korać S, Ćalić D, Flamini G, Kumari BR (2013) High-efficiency Agrobacterium rhizogenes-mediated genetic transformation in Artemisia vulgaris: hairy root production and essential oil analysis. Ind Crops Prod 44:643–652

    Article  CAS  Google Scholar 

  • Thilip C, Raju CS, Varutharaju K, Aslam A, Shajahan A (2015) Improved Agrobacterium rhizogenes-mediated hairy root culture system of Withania somnifera (L.) Dunal using sonication and heat treatment. 3 .Biotech 5:949–956

    Article  PubMed  PubMed Central  Google Scholar 

  • Thuesombat P, Hannongbua S, Akasit S, Chadchawan S (2014) Effect of silver nanoparticles on rice (Oryza sativa L. cv. KDML 105) seed germination and seedling growth. Ecotoxicol Environ Saf 104:302–309

    Article  CAS  PubMed  Google Scholar 

  • Van Nguyen K, Pongkitwitoon B, Pathomwichaiwat T, Viboonjun U, Prathanturarug S (2019) Effects of methyl jasmonate on the growth and triterpenoid production of diploid and tetraploid Centella asiatica (L.) Urb. hairy root cultures. Sci Rep 9:1–8

    Google Scholar 

  • Wang Q, Xing S, Pan Q, Yuan F, Zhao J, Tian Y, Chen Y, Wang G, Tang K (2012) Development of efficient Catharanthus roseus regeneration and transformation system using Agrobacterium tumefaciens and hypocotyls as explants. BMC Biotechnol 12:34

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • White FF, Taylor BH, Huffman GA, Gordon MP, Nester EW (1985) Molecular and genetic analysis of the transferred DNA regions of the root-inducing plasmid of Agrobacterium rhizogenes. J Bacteriol 164:33–44

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wu SQ, Yu XK, Lian ML, Park SY, Piao XC (2014) Several factors affecting hypericin production of Hypericum perforatum during adventitious root culture in airlift bioreactors. Acta Physiol Plant 36:975–981

    Article  CAS  Google Scholar 

  • Yadav P, Jaswal V, Sharma A, Kashyap D, Tuli HS, Garg VK, Das SK, Srinivas R (2018) Celastrol as a pentacyclic triterpenoid with chemopreventive properties. Pharm Pat Anal 7:155–167

    Article  CAS  PubMed  Google Scholar 

  • Yogananth N, Basu MJ (2009) TLC method for the determination of plumbagin in hairy root culture of Plumbago rosea L. Glob J Biotechnol Biochem 4:66–69

    CAS  Google Scholar 

  • Zhang C, Yan Q, Cheuk WK, Wu J (2004) Enhancement of tanshinone production in Salvia miltiorrhiza hairy root culture by Ag+ elicitation and nutrient feeding. Planta Med 70:147–151

    Article  CAS  PubMed  Google Scholar 

  • Zhang B, Zheng LP, Yi Li W, Wen Wang J (2013) Stimulation of artemisinin production in Artemisia annua hairy roots by Ag-SiO2 core–shell nanoparticles. Curr Nanosci 9:363–370

    Article  CAS  Google Scholar 

  • Zhao J, Davis LC, Verpoorte R (2005) Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol Adv 23:283–333

    Article  CAS  PubMed  Google Scholar 

  • Zhao JL, Zhou LG, Wu JY (2010) Effects of biotic and abiotic elicitors on cell growth and tanshinone accumulation in Salvia miltiorrhiza cell cultures. Appl Microbiol Biotechnol 87:137–144

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

We thank CSIR for providing the Senior Research Fellowship to Anil Kumar Moola with the Grant in Aid No. 09/475(0201)/2018-EMR-I.

Author information

Authors and Affiliations

  1. Department of Botany, Bharathidasan University, Tiruchirappalli, 620 024, India

    Anil Kumar Moola, T. Senthil Kumar & B. D. Ranjitha Kumari

  2. Department of Biotechnology, Aditya Degree and PG College, Lakshmi Narayana Nagar, Kakinada, Andhra Pradesh, 533 003, India

    Anil Kumar Moola

Authors
  1. Anil Kumar Moola
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. Senthil Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. D. Ranjitha Kumari
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Anil Kumar Moola.

Ethics declarations

Conflict of interest

The authors stated that no conflict of interest exists.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOC 5376 KB)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Moola, A.K., Senthil Kumar, T. & Ranjitha Kumari, B.D. Enhancement of Celastrol compound by silver nanoparticles and acetosyringone in Celastrus paniculatus Willd. through adventitious and hairy root culture. J. Plant Biochem. Biotechnol. 31, 429–434 (2022). https://doi.org/10.1007/s13562-021-00676-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13562-021-00676-y

Keywords

Search

Navigation