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Ectopic expression of a grape vine vacuolar NHX antiporter enhances transgenic potato plant tolerance to salinity

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Abstract

Salinity is a crucial environmental constraint that reduces plant productivity. However, plants activate different signaling pathways to overcome the abiotic stress. The NHX (Na+/H+ exchanger) antiporter corresponds to one of the antiporters involved in response to salinity. They are known to be responsible for the vacuole compartmentation of toxic Na+. In this report, a grapevine vacuolar antiporter (VvNHX1) cDNA was introduced into potato, response of transgenic plants to salinity was evaluated under in vitro and greenhouse culture conditions. The transgenic plants showed higher growth rate than wild type (WT) after the salinity treatment suggesting an improved tolerance both in vitro and under greenhouse culture conditions. In addition, a lower oxidative stress level was observed while a higher relative water and soluble sugar content were measured in transgenic plants compared to WT plants. Furthermore, in contrast to WT plants, the transgenic plants displayed an increase of leaf ion (K+, Mg2+) content and a decline in Na+ accumulation. The increase in the antioxidant enzyme activities in transgenic plants suggests that they can overcome oxidative stress resulting from salt treatment. The measurement of the tuber yield and the weight loss of plants sprinkled with 100 mM NaCl in the greenhouse showed a low negative effect on transgenic plants (12.5 and 40%) in comparison to WT (80%).

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Abbreviations

ROS:

Reactive oxygen species

NHX:

Na+/H+ antiporter

SOD:

Superoxide dismutase

CAT:

Catalase

GPX:

Gluthatione peroxidase

MDA:

Malondialdehyde

TCA:

Trichloroacetic acid

TBA:

Thiobarbituric acid

RWC:

Relative water content

ANOVA:

Analysis of variance

WT:

Wild type

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Acknowledgements

This work was financed by the Tunisian Ministry of High Education and Scientific Research. The authors thank to Anne-Lise Haenni from the Institute Jacques Monod (France) and Mrs Najoua Neifar english teacher from the University of Sfax-Tunisia and Miss cyrine Bouzid from ODDO BHF for improving the English throughout the manuscript.

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Authors and Affiliations

  1. Laboratory of Plant improvement and Valorization of Agro-ressources, National School of Engineering of Sfax (ENIS), Sfax University, 3038, Sfax, Tunisia

    Safa Charfeddine, Mariam Charfeddine & Radhia Gargouri-Bouzid

  2. Laboratory of Extremophilic Plant, Biotechnology Center of Borj Cedria, Borj Cedria, Tunisia

    Mohsen Hanana

Authors
  1. Safa Charfeddine
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  2. Mariam Charfeddine
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  3. Mohsen Hanana
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  4. Radhia Gargouri-Bouzid
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Contributions

SC and MC carried out the experimental work. The insertion of the VvNHX1 gene into the pCAMBIA99.1 vector was generated by MH. RGB participated in the data analysis and contributed in writing and revising the manuscript.

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Correspondence to Safa Charfeddine.

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Charfeddine, S., Charfeddine, M., Hanana, M. et al. Ectopic expression of a grape vine vacuolar NHX antiporter enhances transgenic potato plant tolerance to salinity. J. Plant Biochem. Biotechnol. 28, 50–62 (2019). https://doi.org/10.1007/s13562-018-0462-x

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  • DOI: https://doi.org/10.1007/s13562-018-0462-x

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