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Metabolic effects of glyphosate on transgenic maize expressing a G2-EPSPS gene from Pseudomonas fluorescens

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Abstract

Transgenic glyphosate-tolerant maize expressing 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene has been commercialized since 1996. However, it is not very clear how glyphosate treatment affects metabolite pathway in transgenic glyphosate-tolerant maize. Here, we obtained numerous of glyphosate-tolerant transgenic maize expressing a Pseudomonas fluorescens G2-EPSPS gene. The expression and integration site of G2-EPSPS in transgenic maize event Aro203, which can tolerate 3 folds of field usage of glyphosate, were investigated. Metabolite analysis was performed with Aro203 leaf samples using GC/MS method. The results showed that total 58 metabolites were identified. Over-expression of G2-EPSPS led to the increase of glutamate, malate, hydroxylamine and trehalose contents, but the decrease of glyoxylate, ribose and sucrose, compared to wild type plants. Twenty-two and 13 metabolites were up-regulated and down-regulated in non-transgenic maize by glyphosate treatments, respectively, whereas fewer metabolites (10 up-regulated and 4 down-regulated) were affected in transgenic maize. Glyphosate treatment significantly stimulated the accumulation of most amino acids but decreased lots of sugars in non-transgenic plants. The PCA analysis results showed that wild type plant cluster treated with glyphosate was clearly separated with other three clusters. The results in this study provide evidence to understand how genetic modification or glyphosate treatment affects the metabolite pathway in maize.

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Abbreviations

EPSPS:

5-enolpyruvylshikimate-3-phosphate synthase

PEP:

Phosphoenolpyruvic acid

S3P:

3-phosphoshikimic acid

rbcS:

rib-1,5-bisphospate carboxylase

GC-MS:

Gas chromatography–mass spectrometry

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Acknowledgments

We thank Dr. Minhui Li from Inner Mongolia University of Science &Technology for the GC/MS analysis. This work was supported by the National Major Project for Transgenic Organism Breeding 2013ZX08010-004.

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Authors and Affiliations

  1. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Zhongguancun South Street 12, Beijing, 100081, China

    Yunjun Liu, Yuwen Zhang, Yan Liu & Guoying Wang

  2. College of Agriculture and Biotechnology, China Agricultural University, Yuanmingyuan West Road 2, Beijing, 100094, China

    Yuwen Zhang

  3. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Zhongguancun South Street 12, Beijing, 100081, China

    Wei Lu

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  1. Yunjun Liu
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  2. Yuwen Zhang
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  3. Yan Liu
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  4. Wei Lu
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  5. Guoying Wang
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Correspondence to Guoying Wang.

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Liu, Y., Zhang, Y., Liu, Y. et al. Metabolic effects of glyphosate on transgenic maize expressing a G2-EPSPS gene from Pseudomonas fluorescens . J. Plant Biochem. Biotechnol. 24, 233–241 (2015). https://doi.org/10.1007/s13562-014-0263-9

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  • DOI: https://doi.org/10.1007/s13562-014-0263-9

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