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Analysis of Arabidopsis genes encoding putative class III lipases

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Abstract

Triacylglycerol lipases are class of enzymes which catalyze the hydrolysis of long-chain triglycerides. They are widely found in the plant kingdom, numerous genes putatively encoding triacylglycerol lipases are sequenced but only a few of them have been characterized. Here we systematically analyzed Arabidopsis gene sequences deposited in public databases, and identified 38 putative class III lipase proteins, all of which contain a highly conserved lipase_3 domain (Pfam ID: PF01764). These 38 genes are randomly distributed on all chromosomes, and their genomic sequences consist of variable numbers of introns from zero to 13. They can be divided into four groups based on homology of protein sequences, and their potential subcellular localization is predicted to cytosol, chloroplast, mitochondria or endoplasmic reticulum. Furthermore, ten typical genes are selected to investigate their expression patterns. Most of them show weak tissue- or organ-specificity expression pattern. Several of them significantly accumulates in some tissues or organs in addition to germinated seedlings. Some of them are specifically transcribed during seed germination while others are not detected during stages of normal growth which are probably induced by stresses. In conclusion, putative Arabidopsis class III lipases display polymorphism in their sequences, gene structures and expression patterns.

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Abbreviations

TAG:

Triacylglycerol

LID:

DAD1-homologous lipase

DAD:

Defective in anther dehiscence protein

AGLIP:

Arabidopsis putative classs III lipase

OBL:

Oil body-associated lipase

PRLIP:

Pathogenesis-related lipase

NTLIP:

lipase_3 N-terminal domain containing lipase

CaMBP:

Calmodulin-binding protein

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Acknowledgements

This work was funded by China National “863” High-Tech Program, China Ministry of Education and Shanghai Science and Technology Committee.

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Authors and Affiliations

  1. State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Morgan-Tan International Center for Life Sciences, Fudan-Manchester Bioinformatics Center, Fudan University, Shanghai, 200433, People’s Republic of China

    Wei Li, Xiaofen Sun & Kexuan Tang

  2. Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, School of Life Science and Technology, Institute of Systems Biology, Shanghai Jiao Tong University, Shanghai, 200030, People’s Republic of China

    Hua Ling, Fei Zhang, Hongyan Yao & Kexuan Tang

Authors
  1. Wei Li
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  2. Hua Ling
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  3. Fei Zhang
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  4. Hongyan Yao
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  5. Xiaofen Sun
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  6. Kexuan Tang
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Correspondence to Kexuan Tang.

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Wei Li and Hua Ling are co-first authors.

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Li, W., Ling, H., Zhang, F. et al. Analysis of Arabidopsis genes encoding putative class III lipases. J. Plant Biochem. Biotechnol. 21, 261–267 (2012). https://doi.org/10.1007/s13562-011-0103-0

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  • DOI: https://doi.org/10.1007/s13562-011-0103-0

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