Abstract
Comparison of morphogenetic potential of three important Indian species of Garcinia—G. indica, G. cambogia and G. xanthochymus has been reported. Apomictic seeds of G. indica were found to be morphogenetically most potential followed by G. cambogia. The explants of G. xanthochymus were highly recalcitrant towards in vitro conditions and failed to induce adventitious buds on any of the media tested. High frequency direct shoot bud differentiation was induced in aseptic seed cultures of G. indica and G. cambogia on MS medium supplemented with cytokinins (BAP, kinetin or TDZ). Amongst the three cytokinins tested, TDZ (0.1–0.5 μM) was most effective for adventitious bud differentiation in both G. indica and G. cambogia, however, the proliferating buds failed to elongate. Substantial number of buds induced on BAP supplemented media elongated into shoots after subculture on elongation medium. Addition of NAA along with cytokinins in the induction medium enhanced callusing without improvement in bud induction response. The induced adventitious buds were elongated on MS basal medium containing 0.2% activated charcoal. Direct rooting was achieved in both G. indica and G. cambogia on auxin supplemented media with best response at 10 μM IBA concentration in both the species. The in vitro raised plantlets showed 90% survival in the field when transferred after hardening and acclimatization.
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Abbreviations
- BAP:
-
6- Benzylaminopurine
- IBA:
-
Indole-3-butyric acid
- MS:
-
Murashige and Skoog’s medium (1962)
- NAA:
-
α-Napthalene acetic acid
- TDZ:
-
Thidiazuron
References
Deshpande RS, Chavan SS, Dhonukshe BL (1999) In vitro shoot regeneration in kokum (Garcinia indica L). Ann Plant Physiol 13:31–34
Goh CJ, Ng SK, Prakash L, Loh CS (1997) The role of ethylene on direct shoot bud regeneration from mangosteen (Garcinia mangostana L) leaves cultured in vitro. Plant Sci 124:193–202
Goh CJ, Prakash L, Loh CS (1994) High frequency direct shoot bud regeneration from excised leaves of mangosteen (Garcinia mangostana L) trees. Plant Sci 101:173–180
Goh HKL, Rao AN, Loh CS (1990) Direct shoot bud formation from leaf explant of seedlings and mature mangosteen (Garcinia mangostana L) trees. Plant Sci 68:113–121
Huang LC, Huang BL, Wang CH, Kuo CI, Murashige T (2000) Developing an improved in vitro propagation system for slow-growing species using Garcinia mangostana L (mangosteen). In Vitro Cell Dev Biol—Plant 36:501–504
Kalia RK, Arya S, Kalia S, Arya ID (2007) Plantlet regeneration from fascicular buds on seedling explants of Pinus roxburghii sarg. Biol Plant 51:653–659
Kulkarni MD, Deodhar MA (2002) In vitro regeneration and hydroxycitric acid production in tissue cultures of Garcinia indica Choisy. Indian J Biotech 1:301–304
Malik SK, Chaudhury R, Kalia RK (2005a) In vitro multiplication and conservation of Garcinia indica: a medicinal tropical tree. Sci Hort 106:539–553
Malik SK, Chaudhury R, Abraham Z (2005b) Seed morphology and germination characteristics in three Garcinia species. Seed Sci Technol 33:595–604
Malik SK, Chaudhury R, Abraham Z (2005c) Desiccation—freezing sensitivity and longevity in seeds of Garcinia indica G. cambogia and G. xanthochymus. Seed Sci Technol 33:723–732
Mathew KM, Rao YS, Kuruvilla KM, Lakshmanan R, George GL, Madhusoodanan KJ, Potty SN (2001) Multiple shoot regeneration in kokum and camboge. J Spices Aromatic Crops 10:151–152
Normah MN, Noor-Azza AB, Aliudin R (1995) Factors affecting in vitro shoot proliferation and ex vitro establishment of mangosteen. Plant Cell Tissue Organ Cult 43:291–294
Normah MN, Rosnah H, Noor-Azza AB (1992) Multiple shoots and callus formation from seeds of mangosteen (Garcinia mangostana L) cultured in vitro. Acta Hort 292:87–90
Prakash L, Ng SK, Loh CS, Goh CJ (1997) Auxin, cytokinins and ethylene differentially regulate specific developmental states associated with shoot bud morphogenesis in leaf tissues of mangosteen (Garcinia mangostana L) cultured in vitro. Plant Cell Physiol 38:59–64
Te-Chato S, Lim M (1999) Plant regeneration of mangosteen via nodular callus formation. Plant Cell Tissue Organ Cult 59:89–93
Te-Chato S, Lim M (2000) Improvement of mangosteen micropropagation through meristematic nodular callus formation from in vitro derived leaf explants. Sci Hort 86:291–298
Teo CKH (1992) In vitro culture of mangosteen seed. Acta Hort 292:81–85
Wealth of India (1980) The Wealth of India—A dictionary of Indian raw materials and industrial products (Raw materials Series Vol 4). NISCOM Council of Scientific and Industrial Research, New Delhi, India, pp 101–103
Zaerr JB, Mapes MO (1982) Action of growth regulators. In: Bonga JM, Durzan DJ (eds) Tissue Culture in Forestry. Martinus Nijhoff/ Dr. W. Junk Publishers, The Hague-Boston-London, pp 231–255
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Kalia, R.K., Malik, S.K. & Chaudhury, R. In vitro morphogenetic studies on three apomictic species of Garcinia . J. Plant Biochem. Biotechnol. 21, 279–285 (2012). https://doi.org/10.1007/s13562-011-0085-y
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DOI: https://doi.org/10.1007/s13562-011-0085-y