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Molecular markers linked with bruchid resistance in Vigna radiata var. Sublobata and their validation

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Abstract

Bruchid, Callosobruchus chinensis (L.) is an important pest of Vigna radiata during storage. RFLP and PCR based markers identified, linked with bruchid resistance gene in wild accession of greengram (V. radiata var. Sublobata) either collected from Madagaskar or Australia. Whether these markers will be useful for marker assisted introgression of bruchid resistance gene from the Indian accession into the existing cultivars are not known. Here, we employed two STS based markers which were found earlier, to be linked with bruchid resistance gene in Australian accession ACC41. Only one primer pair, STSbr1 showed polymorphism among Indian Sublobata accession (Sub2) and other twelve green gram cultivars. Analysis of 113 segregating lines (F6) of a cross between a popular cultivar of West Bengal, B1 and Sub2 showed a cent percent co-segregation of resistant locus with the polymorphic fragment. STSbr1 behave as a dominant marker among Indian genotypes although it has been shown earlier a co-dominant banding pattern between ACC41 and other Australian Susceptible cultivars. Other STS marker, STSbr2, does not produce any polymorphic fragment among Sub2 and 18 greengram genotypes. STSbr1 employed in screening of 50 green gram accessions and found high efficiency in screening of bruchid resistant genotypes also. So STSbr1 will be useful for marker assisted selection and germplasm screening for development of bruchid resistant greengram.

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Abbreviations

BAC:

Bacterial Artificial Chromosome

bp:

Base pairs

NBPGR:

National Bureau of Plant Genetic Resources

dNTPs:

Deoxynucleotide Tri Phosphates

PCR:

Polymerase Chain Reaction

RFLP:

Restriction Fragment Length Polymorphism

SSR:

Simple Sequence Repeat

STS:

Sequence Tag Site

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Acknowledgement

We sincerely thank to ICAR-Niche area excellence programme, ‘Arsenic management options including organic agricultural systems in West Bengal’ for financial support.

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Authors and Affiliations

  1. Department of Genetics and Crop Research Unit, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, WB, 741252, India

    Sutanu Sarkar, Swagata Ghosh, Mitali Chatterjee, Padminee Das, Triparna Lahari, Anirban Maji, Nirmal Mondal, Kanti Kumar Pradhan & Somnath Bhattacharyya

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  1. Sutanu Sarkar
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  2. Swagata Ghosh
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  3. Mitali Chatterjee
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  4. Padminee Das
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  5. Triparna Lahari
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  7. Nirmal Mondal
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  9. Somnath Bhattacharyya
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Corresponding author

Correspondence to Somnath Bhattacharyya.

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Validation of a set of greengram genotypes by bruchid resistance linked STSbr1 marker in 2% agarose gel, amplification of 225bp fragment as per resistant parent is indicated by arrow; as per feeding assay resistant genotypes, R and susceptible, S are marked below (GIF 647 kb)

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Sarkar, S., Ghosh, S., Chatterjee, M. et al. Molecular markers linked with bruchid resistance in Vigna radiata var. Sublobata and their validation. J. Plant Biochem. Biotechnol. 20, 155–160 (2011). https://doi.org/10.1007/s13562-011-0039-4

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  • DOI: https://doi.org/10.1007/s13562-011-0039-4

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