Abstract
A great deal of attention has been focused on anionic nanoclays for industrial applications such as catalyst, filters, additives, plastics, and pharmaceutics due to their high adsorption ability, large surface area, and high ion exchange capacity. However, their potential toxicological effects on human health were not yet completely determined, especially by employing several cytotoxicity assays in cell lines. In this study, we prepared two different forms of anionic nanoclays, carbonate and chloride forms, and evaluated their cytotoxicity in terms of cell proliferation and viability in short- and long-term, respectively. The results demonstrated that both different forms of anionic nanoclays did not acutely affect cell proliferation and viability of human lung cells after 72 h. However, the cytotoxicity of the present nanoparticles differed with the testing method used in short-term, underlining the importance of appropriate cytotoxicity assay employed. In long-term, both nanoparticles at high concentration (250–500 μg/mL) inhibited colony formation after 10 days, suggesting their potential chronic toxicity.
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Chung, HE., Kim, IS., Baek, M. et al. Long-term cytotoxicity potential of anionic nanoclays in human cells. Toxicol. Environ. Health Sci. 3, 129–133 (2011). https://doi.org/10.1007/s13530-011-0088-z
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DOI: https://doi.org/10.1007/s13530-011-0088-z