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MicroRNA-155 in serum-derived extracellular vesicles as a potential biomarker for hematologic malignancies - a short report

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Abstract

Purpose

The use of extracellular vesicles (EVs) from body fluids as “liquid biopsies” is emerging as a promising approach for the diagnosis, prognosis and therapeutic monitoring of cancer patients. MicroRNA-155 (miR155), a non-coding transcript of the B-cell integration cluster (BIC) gene, has been reported to play a critical role in the pathogenesis of several types of hematologic malignancies (HMs) in which high miR155 levels have been found. At yet, however, the EV miR155 level and its putative clinical relevance in sera of HM patients have not been reported.

Methods

EVs from sera of representative patients with eight different HMs and healthy subjects (controls) were isolated using differential centrifugation. The identity and quality of the EVs were verified by atomic force and transmission electron microscopy. The EV miR155 levels were measured by quantitative RT-PCR. The sensitivity, specificity and area under the curve (AUC) of differences in EV miR155 levels were determined using ROC curve analyses.

Results

We found that the EV miR155 levels were significantly higher in chronic lymphocytic leukemia (CLL), acute myeloid leukemia (AML) and Waldenström’s macroglobulinemia (WM) cases compared to controls. Conversely, we found that the EV miR155 levels were significantly lower in myelodysplastic syndrome (MDS) and multiple myeloma (MM) cases. No differences were found in follicular lymphoma (FL), diffuse large B-cell lymphoma (DLBCL) or Hodgkin’s Lymphoma (HL) cases compared to controls. EV miR155 ROC curve analyses revealed significantly different patterns in CLL and AML cases compared to controls, and in AML cases compared to MDS cases (p = 0.004, p = 0.01 and p = 0.04, respectively). In addition, we found that high EV miR155 levels correlated with high white blood cell counts in AML patients.

Conclusion

Our data indicate that EV miR155 may serve as an attractive new, non-invasive diagnostic biomarker in human hematologic malignancies.

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Author information

Authors and Affiliations

  1. Laboratory of Preclinical and Translational Research, IRCCS-Referral Cancer Center of Basilicata (CROB), 85028, Rionero in Vulture, Potenza, Italy

    Antonella Caivano, Francesco La Rocca, Vittorio Simeon, Ilaria Laurenzana, Luciana De Luca & Stefania Trino

  2. Institute for the Study of the Structure of Matter, National Research Council (CNR), Rome, Italy

    Marco Girasole & Simone Dinarelli

  3. Institute for Sustainable Plant Protection, CNR, Bari, Italy

    Angelo De Stradis

  4. Unit of Clinical Pathology, IRCCS-CROB, Rionero in Vulture, Potenza, Italy

    Antonio Traficante

  5. Department of Onco-Hematology, IRCCS-CROB, Rionero in Vulture, Potenza, Italy

    Giovanni D’Arena, Giovanna Mansueto, Oreste Villani & Giuseppe Pietrantuono

  6. Department of Hematology, Catholic University of the Sacred Heart, Rome, Italy

    Luca Laurenti

  7. CEINGE-Biotecnologie Avanzate scarl and Department of Molecular Medicine and Medical Biotechnologies, Federico II University, Naples, Italy

    Luigi Del Vecchio

  8. Scientific Direction, IRCCS-CROB, Rionero in Vulture, Potenza, Italy

    Pellegrino Musto

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  1. Antonella Caivano
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  2. Francesco La Rocca
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  3. Vittorio Simeon
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  4. Marco Girasole
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  11. Giovanni D’Arena
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  13. Oreste Villani
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  14. Giuseppe Pietrantuono
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  15. Luca Laurenti
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Corresponding author

Correspondence to Antonella Caivano.

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This is an original article that has not been previously published and has not been submitted for publication elsewhere.

Conflict of interest

The authors declare no conflict of interest.

This study involved human partecipants.

Informed consent

All patients and heathy subjects signed an informed consent form. We followed guidelines of the Helsinki Declaration and experiments were approved by the Ethics Committee of IRCCS-CROB (Prot 3725; 7–2-2008).

Additional information

Luigi Del Vecchio and Pellegrino Musto contributed equally to this work.

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Caivano, A., La Rocca, F., Simeon, V. et al. MicroRNA-155 in serum-derived extracellular vesicles as a potential biomarker for hematologic malignancies - a short report. Cell Oncol. 40, 97–103 (2017). https://doi.org/10.1007/s13402-016-0300-x

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