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Differential expression of miR-139, miR-486 and miR-21 in breast cancer patients sub-classified according to lymph node status

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Abstract

Purpose

Therapeutic decisions in breast cancer are increasingly guided by prognostic and predictive biomarkers. Non-protein-coding microRNAs (miRNAs) have recently been found to be deregulated in breast cancers and, in addition, to be correlated with several clinico-pathological features. One of the most consistently up-regulated miRNAs is miR-21. Here, we specifically searched for differentially expressed miRNAs in high-risk breast cancer patients as compared to low-risk breast cancer patients. In the same patients, we also compared miR-21 expression with the expression of its presumed target PTEN.

Methods

Both microarray and RT-qPCR techniques were used to assess miRNA expression levels in lymph node-positive and -negative human invasive ductal carcinoma tissues. Simultaneously, PTEN protein expression levels were assessed using immunohistochemistry.

Results

miR-486-5p and miR-139-5p were found to be down-regulated in patients with lymph node metastases, whereas miR-21 was found to be up-regulated in patients with a positive lymph node status. miR-21 expression levels were found to significantly correlate with tumour size (r = 0.403, p = 0.009; Spearman’s rank), whereas no relation was found between miR-21 and PTEN expression levels (Kruskal-Wallis test).

Conclusion

Down-regulation of miR-486-5p and miR-139-5p, in conjunction with up-regulation of miR-21, may represent a useful signature for the identification of high-risk breast cancer patients.

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Acknowledgments

We thank Stine Jørgensen and Jette Nyvang Jørgensen for their help with patient records and tissue sample collections, as well as Mel Charles John Heeran for skilful PTEN staining of the tissue sections. Birgit Hertz, Marianne Fregil and Gitte Friis are acknowledged for their excellent technical assistance in the laboratory. Moreover, we thank Søren Møller and Exiqon for performing the microarray experiments. We are grateful to Catherine Aubel for her careful and professional proofreading of the manuscript. Furthermore, we are indebted to Susanne Husted and Boye Schnack Nielsen for qPCR discussion and antibody selection, respectively. We thank the anonymous Cellular Oncology referees for constructive comments and comprehensive proofreading. The Danish CancerBiobank (DCB) and the MAMBIO breast carcinoma biobank are acknowledged for biological material and for information regarding handling and storage. The work was funded by the Danish Graduate School in Clinical Oncology and the Research Foundation of Copenhagen County.

Conflict of interest

During the experiments performed for this manuscript Thomas Litman and Rolf Søkilde were employed full-time for an entity (Exiqon A/S) having a commercial interest in the microarray platform used.

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Author notes

  1. Lene Rask

    Present address: Department of Clinical Neurophysiology and Center for Healthy Aging, University of Copenhagen and Glostrup Hospital, Entrance 3, 9th floor, Nordre Ringvej 57, 2600, Glostrup, Denmark

  2. Rolf Søkilde

    Present address: Department of Oncology, University of Lund, Lund, Sweden

  3. Jens Eriksen

    Present address: Department of Pathology, Næstved Sygehus, Ringstedgade 61, 4700, Næstved, Denmark

  4. Thomas Litman

    Present address: Leo Pharma, Molecular Biomedicine, Industriparken 55, 2750, Ballerup, Denmark

Authors and Affiliations

  1. Department of Oncology 54O5, Herlev Hospital, University of Copenhagen, Herlev Ringvej 75, 2730, Herlev, Denmark

    Lene Rask & Jens Eriksen

  2. Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3B, 2200, København N, Denmark

    Lene Rask

  3. Department of Pathology, Herlev hospital, University of Copenhagen, Herlev Ringvej 75, 2730, Herlev, Denmark

    Eva Balslev & Estrid Høgdall

  4. Department of Breast Surgery, Herlev Hospital, University of Copenhagen, Herlev Ringvej 75, 2730, Herlev, Denmark

    Henrik Flyger

  5. Exiqon A/S, Bygstubben 9, 2950, Vedbaek, Denmark

    Rolf Søkilde & Thomas Litman

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Rask, L., Balslev, E., Søkilde, R. et al. Differential expression of miR-139, miR-486 and miR-21 in breast cancer patients sub-classified according to lymph node status. Cell Oncol. 37, 215–227 (2014). https://doi.org/10.1007/s13402-014-0176-6

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