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Ultrasonic enhancement of lipase-catalyzed transesterification for biodiesel production from used cooking oil

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Abstract

This work investigated the ultrasonic irradiation-enhanced transesterification using lipase from the viscera of Nile tilapia (Oreochromis niloticus) to produce biodiesel from used cooking oil (UCO) and methanol. The effects of processing conditions such as irradiation time, ultrasonic frequency, and temperature on biodiesel yield were investigated. Results showed that the use of ultrasound decreased the reaction time from 28 to 3 h with ultrasonic frequency of 16 kHz, methanol to oil molar ratio of 4:1, lipase concentration of 30 kUnit, and reaction temperature of 40 °C. The efficacy of ultrasound was compared with conventional mechanical stirring operated at optimum conditions, and the ultrasonic irradiation coupled with stirring enhanced the transesterification with the highest yield of 97.59%. The fuel properties of the produced biodiesel in this study desirably met the recommended biodiesel standards as prescribed by EN 14214 and ASTM D 6751, suggesting the biodiesel obtained from ultrasound-assisted lipase-catalyzed transesterification of UCO with methanol is a promising alternative for conventional biodiesels and diesels.

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Funding

This research was supported by the National Research Council of Thailand, Thailand, as of fiscal year 2019 and Thaksin University, Thailand.

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Authors and Affiliations

  1. Biotechnology Program, Faculty of Agro and Bio Industry, Thaksin University, Phatthalung Campus, Phatthalung, 93210, Thailand

    Jaran Patchimpet

  2. Department of Food Science and Agricultural Chemistry, McGill University, Macdonald Campus, Sainte-Anne-de-Bellevue, QC, H9X 3V9, Canada

    Yi Zhang & Benjamin K. Simpson

  3. College of Food Science and Technology, Nanjing Agricultural University, Jiangsu, 2010095, People’s Republic of China

    Xin Rui

  4. Department of Chemistry, Faculty of Science, Thaksin University, Phatthalung Campus, Phatthalung, 93210, Thailand

    Kanokphorn Sangkharak

  5. College of Nanotechnology, King Mongkut’s Institute of Technology Ladkrabang, Chalongkrung Rd., Ladkrabang, Bangkok, 10520, Thailand

    Apiluck Eiad-ua

  6. Department of Food Science and Technology, Faculty of Agro and Bio Industry, Thaksin University, Phatthalung Campus, Phatthalung, 93210, Thailand

    Sappasith Klomklao

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  1. Jaran Patchimpet
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  2. Yi Zhang
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  4. Xin Rui
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  6. Apiluck Eiad-ua
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Contributions

Jaran Patchimpet: investigation, methodology, and writing — original draft. Yi Zhang: methodology and writing — revision. Benjamin K. Simpson: writing — review and editing. Xin Rui: methodology. Kanokphorn Sangkharak: software. Apiluck Eiad-ua: laboratory analysis. Sappasith Klomklao: conceptualization, investigation, and writing — review and editing.

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Correspondence to Sappasith Klomklao.

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Highlights

• Enzyme-catalyzed biodiesel synthesis from used cooking oil using ultrasonication.

• With ultrasonic irradiation, 97.59% yield was obtained in 3 h.

• Ultrasound significantly reduced reaction time as compared to conventional method.

• Ultrasonic method gave significant benefits than conventional stirring method.

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Patchimpet, J., Zhang, Y., Simpson, B.K. et al. Ultrasonic enhancement of lipase-catalyzed transesterification for biodiesel production from used cooking oil. Biomass Conv. Bioref. 13, 8151–8160 (2023). https://doi.org/10.1007/s13399-021-01790-6

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  • DOI: https://doi.org/10.1007/s13399-021-01790-6

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