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Response surface methodology for enzymatic hydrolysis optimization of jabon alkaline pulp with Tween 80 surfactant addition

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Abstract

This study was intended to determine the optimum condition for enzymatic hydrolysis of the jabon wood pulp. Ethanol production with separate hydrolysis and fermentation and simultaneous saccharification and fermentation processes were also compared. Jabon was delignified with 25% (w/v) NaOH and the pulp was beaten to a freeness of 600, 400 and 200 mL CSF. Tween 80 surfactant was used to improve the enzymatic hydrolysis. The effect of pulp freeness, enzyme loading, and surfactant concentration on enzymatic hydrolysis was optimized by response surface method according to the Box-Behnken design. Under optimized condition (550 mL CSF pulp freeness, 28 FPU/g enzyme loading and 1.66% Tween 80), the yield of reducing sugar per oven-dry weight (g) of biomass was eight times higher than that of the untreated ones. The ethanol yields improved up to 24%. Separate hydrolysis and fermentation process resulted in a higher ethanol yield. The surfactant addition increases the conversion of cellulose into bioethanol by 27%.

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Acknowledgements

The authors gratefully acknowledge the Directorate General of Higher Education, the Ministry of National Education of the Republic of Indonesia (BPPDN DIKTI) for the master’s degree scholarship awarded to Martua Yan Steward Nababan in 2013. The authors are also thankful to Research Center for Biomaterial, Indonesian Institute of Sciences (LIPI) for the research facilities. Special thanks are addressed to Sita Heris Anita, M. Si, and Maulida Oktaviani, S. Si for valuable discussion on technical enzymatic hydrolysis and fermentation stage.

Funding

This study was funded by The Directorate of Research and Community Service-Ministry of Research, Technology and Higher Education of the Republic of Indonesia under contract number of 079/SP2H/LT/DRPM/II/2016.

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Authors and Affiliations

  1. Department of Forest Products, Faculty of Forestry - IPB University, Jl. Lingkar Akademik Kampus IPB Darmaga, Bogor, 16680, Indonesia

    Martua Yan Steward Nababan & Nyoman J Wistara

  2. Research Center for Biomaterial, Indonesian Institute of Sciences (LIPI), Bogor, Indonesia

    Widya Fatriasari

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  1. Martua Yan Steward Nababan
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Correspondence to Nyoman J Wistara.

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Nababan, M.Y.S., Fatriasari, W. & Wistara, N.J. Response surface methodology for enzymatic hydrolysis optimization of jabon alkaline pulp with Tween 80 surfactant addition. Biomass Conv. Bioref. 12, 2165–2174 (2022). https://doi.org/10.1007/s13399-020-00807-w

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