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Metabolic profile of mixed culture acidogenic fermentation of lignocellulosic residues and the effect of upstream substrate fractionation by steam explosion

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Abstract

Lignocellulosic biomass residues have attracted attention for the sustainable production of molecules for material and energetic use through biochemical conversion. Their recalcitrant structure prevents a broader use and asks for the development of sustainable techniques that can efficiently separate, recover and valorize the constituting components. In a cascading concept, residual streams of such processes can be further exploited in an attempt to valorize the largest possible fraction of the initial material. Three lignocellulosic substrates, namely dried sugar beet pulp, wheat bran and miscanthus straw, were upstream fractionated by steam explosion to extract the hemicellulose fraction. This study evaluated the valorization of the residual solid fraction through mixed acidogenic fermentation for the production of volatile fatty acids (VFA) as platform chemicals. Batch experiments have been conducted for the reference material (non-treated) and the solid fraction remaining after steam explosion, with and without the addition of an external mixed inoculum. Steam explosion residues contained less hemicellulose than the initial materials. The difference in the fermentation profile between steam explosion residues and non-treated substrates is dependent on the substrate. Maximum total VFA (tVFA) concentration was 18.8 gCOD/kgmixed_liquor, and maximum yield of chemical oxygen demand (COD) conversion into tVFA was 33 % for the case of non-treated inoculated beet pulp.

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Abbreviations

W:

Wheat bran

B:

Dried sugar beet pulp

M:

Miscanthus straw

VFA:

Volatile fatty acids

tVFA:

Total VFA

CODs:

Soluble chemical oxygen demand

CODt:

Total chemical oxygen demand

ML:

Mixed liquor

TS:

Total solids

VS:

Volatile solids

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Acknowledgments

The authors thank the Walloon Region for financial support in the frame of the LIGNOFUEL and WAL-AID programs (convention no. 716721 and 6089, respectively). We thank Matthieu Leclercq for his technical assistance.

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Authors and Affiliations

  1. Bioengineering Group, Earth and Life Institute-Applied Microbiology, Université catholique de Louvain, Croix du Sud 2, L7.05.19, 1348, Louvain-la-Neuve, Belgium

    Anastasios Perimenis, Ingrid M. van Aarle, Thomas Nicolay & Patrick A. Gerin

  2. Unit of Biological and Industrial Chemistry, Gembloux Agro-Bio Tech, Université de Liège, Passage des Déportés 2, 5030, Gembloux, Belgium

    Nicolas Jacquet, Laurence Meyer & Aurore Richel

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  1. Anastasios Perimenis
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  2. Ingrid M. van Aarle
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Correspondence to Patrick A. Gerin.

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Perimenis, A., van Aarle, I.M., Nicolay, T. et al. Metabolic profile of mixed culture acidogenic fermentation of lignocellulosic residues and the effect of upstream substrate fractionation by steam explosion. Biomass Conv. Bioref. 6, 25–37 (2016). https://doi.org/10.1007/s13399-015-0164-8

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  • DOI: https://doi.org/10.1007/s13399-015-0164-8

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