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Production and purification of keratinase using chicken feather bioconversion by a newly isolated Aspergillus fumigatus TKF1: detection of valuable metabolites

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Abstracts

The strain Aspergillus fumigatus TKF1 (Genbank-JQ268555), isolated from soil of poultry waste site, produced extracellular keratinase in basal medium [0.05 % K2HPO4, 0.025 % MgSO4, 0.02 % CaCO3, 0.5 % NaCl and 0.015 % FeSO4 (w/v)] at 30 °C in pH 6 with 0.5 % supplementation of raw chicken feather after 84 h. Further statistical modeling (response surface methodology) was applied to optimized condition for keratinase production was followed by 84.33 h fermentation time at 30.2 °C and 0.74 % (w/v) feather supplement with initial pH 6.4. The response surface data and parametric optimization of the fermentation medium (one variable at a time) both condition satisfactorily gave 2.5-fold higher production than its unoptimized state. This enzyme preparation could disintegrate whole chicken feathers and reduced of disulphide bridges were also detected, increasing with incubation time. The purified keratinase was 24.3-kDa molecular weight, monomer in nature, and exhibited a significant stability and compatibility with temperature and pH. Feather degradation lead to an increase in oligopeptides and free amino acids such as cystein, therionine, phenylalanine, leucine, valine, and isoleucine. Moreover, methionine and phenylalanine were also produced as microbial metabolites. The importance of these finding is appropriate for future applications in agricultural and pharmaceutical industries.

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Acknowledgment

The authors are thankful to the University Grant Commission [sanction no. F.11-114/2008 (BRS)], Government of India, India, for the financial assistance. The assistance with HPLC and MALDI-TOF MS analysis by Dr. Santi Mohan Mandal, IIT-Khargpur, India, is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Microbiology, Vidyasagar University, Midnapore, 721102, West Bengal, India

    Tanmay Paul, Arpan Das, Suman K Halder, Pradeep K. DasMohapatra, Bikas R. Pati & Keshab C. Mondal

  2. Department of Microbiology, Raja N. L. Khan women’s College, Midnapore, 721102, West Bengal, India

    Arpita Mandal

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  1. Tanmay Paul
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  2. Arpan Das
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Correspondence to Keshab C. Mondal.

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Paul, T., Das, A., Mandal, A. et al. Production and purification of keratinase using chicken feather bioconversion by a newly isolated Aspergillus fumigatus TKF1: detection of valuable metabolites. Biomass Conv. Bioref. 4, 137–148 (2014). https://doi.org/10.1007/s13399-013-0090-6

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