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Investigation of simian virus 40 (SV40) and human JC, BK, MC, KI, and WU polyomaviruses in glioma

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Abstract

The gliomagenesis remains not fully established and their etiological factors still remain obscure. Polyomaviruses were detected and involved in several human tumors. Their potential implication in gliomas has been not yet surveyed in Africa and Arab World. Herein, we investigated the prevalence of six polyomaviruses (SV40, JCPyV, BKPyV, MCPyV, KIPyV, and WUPyV) in 112 gliomas from Tunisian patients. The DNA sequences of polyomaviruses were examined by PCR assays. Viral infection was confirmed by DNA in situ hybridization (ISH) and/or immunohistochemistry (IHC). The relationships between polyomavirus infection and tumor features were evaluated. Specific SV40 Tag, viral regulatory, and VP1 regions were identified in 12 GBM (10.7%). DNA ISH targeting the whole SV40 genome and SV40 Tag IHC confirmed the PCR findings. Five gliomas yielded JCPyV positivity by PCR and DNA ISH (2.7%). However, no BKPyV, KIPyV, and WUPyV DNA sequences were identified in all samples. MCPyV DNA was identified in 30 gliomas (26.8%). For GBM samples, MCPyV was significantly related to patient age (p = 0.037), tumor recurrence (p = 0.024), and SV40 (p = 0.045) infection. No further significant association was identified with the remaining tumor features (p > 0.05) and patient survival (Log Rank, p > 0.05). Our study indicates the presence of SV40, JCPyV, and MCPyV DNA in Tunisian gliomas. Further investigations are required to more elucidate the potential involvement of polyomaviruses in these destructive malignancies.

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Acknowledgments

We thank Mrs. Intissar Klibi Toumi and Mrs. Souhir Hassayoun for their technical assistance.

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Authors and Affiliations

  1. Pathology Department, Farhet Hached University Hospital, 4000, Sousse, Tunisia

    Sarra Limam, Ahlem Bdioui, Mohamed Taher Yacoubi & Moncef Mokni

  2. Faculty of Sciences and Techniques of Sidi Bouzid, Kairouan University, Kairouan, Tunisia

    Nabiha Missaoui

  3. Neurosurgery Department, Sahloul University Hospital, 4000, Sousse, Tunisia

    Hedi Krifa

  4. Laboratory of Bioresources, Integrative Biology and Exploiting, ISB, 5000, Monastir, Tunisia

    Boulbeba Selmi

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  1. Sarra Limam
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  2. Nabiha Missaoui
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Contributions

S.L. conceived the work, designed, and performed the experiments; N.M. conceived the work, analyzed data, and wrote the draft of the manuscript; A.B., M.T.Y., H.K., and M.M. provide clinical, surgical, and pathological data. S.L. and N.M. revised the manuscript for the critical scientific content; all the authors read and approved the manuscript. The study was supervised by M.M.

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Correspondence to Nabiha Missaoui.

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This survey was approved by the local Human Ethics Committee at Farhet Hached University Hospital and it conformed to the provisions of Helsinki Declaration.

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Limam, S., Missaoui, N., Bdioui, A. et al. Investigation of simian virus 40 (SV40) and human JC, BK, MC, KI, and WU polyomaviruses in glioma. J. Neurovirol. 26, 347–357 (2020). https://doi.org/10.1007/s13365-020-00833-4

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