Abstract
We screened for viral DNA in cerebrospinal fluid samples using next-generation sequencing (NGS) technology to diagnose CNS viral infections. We collected CSF samples from four cases with clinically suspected viral meningoencephalitis. DNA extracted from the samples was analyzed with NGS, and the results were further validated using PCR. Herpes simplex virus 1 (HSV-1) was detected in the CSF of two patients, HSV-2 and human herpes virus type 3 (HHV-3, VZV) in the CSF of two other patients separately. The number of unique reads of the identified viral genes ranged from 144 to 44205 (93.51 to 99.57 %). The coverage of identified viral genes ranged from 12 to 98 % with a depth value of 1.1 to 35, respectively. The results were further confirmed using PCR in three cases. The clinical presentation and outcomes of these four cases were consistent with the diagnostic results of NGS. NGS of CSF samples can be used as a diagnostic assay for CNS viral infection. Its further application for “pan-viral” or even “pan-microbial” screening of CSF might influence the diagnosis of CNS infectious diseases.
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Abbreviations
- CSF:
-
Cerebrospinal fluid
- NGS:
-
Next-generation sequencing
- EEG:
-
Electroencephalogram
- HSV:
-
Human herpes virus
- VZV:
-
Varicella zoster virus
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Authors’ contributions
Hongzhi Guan, MD, is the principal investigator who drafted the original manuscript. Ao Shen, PhD, participated in laboratory analysis of CSF and drafted parts of the original manuscript. Xia Lv, MD, was involved in case and sample collection as well as analysis or interpretation of data. Xunzhe Yang, MD, was involved in case and sample collection, analysis, or interpretation of the data. Haitao Ren, BA, Yanhuan Zhao, BA, Yinxin Zhang, PhD, and Yanping Gong, PhD, participated in the laboratory analysis of CSF. Peixiang Ni, PhD, analyzed or interpreted the data. Honglong Wu, PhD, Yicheng Zhu, MD, and Liying Cui, MD, conceptualized the study and revised the manuscript.
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We declare that we have no financial and personal relationships with other people or organizations that may inappropriately influence our work. There is no professional or other personal interest of any nature or kind in any product, service, and/or company that could be construed as influencing the position presented in or the review of the manuscript.
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Hongzhi Guan and Ao Shen contributed equally to this work.
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Guan, H., Shen, A., Lv, X. et al. Detection of virus in CSF from the cases with meningoencephalitis by next-generation sequencing. J. Neurovirol. 22, 240–245 (2016). https://doi.org/10.1007/s13365-015-0390-7
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DOI: https://doi.org/10.1007/s13365-015-0390-7