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Structural Distinction of Diacyl-, Alkylacyl, and Alk-1-Enylacyl Glycerophosphocholines as [M – 15]– Ions by Multiple-Stage Linear Ion-Trap Mass Spectrometry with Electrospray Ionization

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Journal of The American Society for Mass Spectrometry

Abstract

We describe a linear ion-trap (LIT) multiple-stage (MSn) mass spectrometric approach towards differentiation of alkylacyl, alk-1-enylacyl- and diacyl-glycerophoscholines (PCs) as the [M – 15]– ions desorbed by electrospray ionization (ESI) in the negative-ion mode. The MS4 mass spectra of the [M – 15 – R2′CH = CO]– ions originated from the three PC subfamilies are readily distinguishable, resulting in unambiguous distinction of the lipid classes. This method is applied to two alkyl ether rich PC mixtures isolated from murine bone marrow neutrophils and kidney, respectively, to explore its utility in the characterization of complex PC mixture of biological origin, resulting in the realization of the detailed structures of the PC species, including various classes and many minor isobaric isomers.

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Acknowledgments

The authors acknowledge support for this research by US Public Health Service grants P41-GM103422, P60-DK-20579, P30-DK56341, DK076729, DK088083, and K99 DK094874.

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Authors and Affiliations

  1. Mass Spectrometry Resource, Washington University School of Medicine, St. Louis, MO, 63110, USA

    Fong-Fu Hsu & John Turk

  2. Division of Endocrinology, Metabolism, and Lipid Research, Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO, 63110, USA

    Fong-Fu Hsu, Irfan J. Lodhi, John Turk & Clay F. Semenkovich

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  1. Fong-Fu Hsu
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  2. Irfan J. Lodhi
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  3. John Turk
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  4. Clay F. Semenkovich
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Correspondence to Fong-Fu Hsu.

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Hsu, FF., Lodhi, I.J., Turk, J. et al. Structural Distinction of Diacyl-, Alkylacyl, and Alk-1-Enylacyl Glycerophosphocholines as [M – 15]– Ions by Multiple-Stage Linear Ion-Trap Mass Spectrometry with Electrospray Ionization. J. Am. Soc. Mass Spectrom. 25, 1412–1420 (2014). https://doi.org/10.1007/s13361-014-0908-x

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  • DOI: https://doi.org/10.1007/s13361-014-0908-x

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