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Comparison of Proteins in Whole Blood and Dried Blood Spot Samples by LC/MS/MS

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Journal of The American Society for Mass Spectrometry

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Abstract

Dried blood spot (DBS) sampling methods are desirable for population-wide biomarker screening programs because of their ease of collection, transportation, and storage. Immunoassays are traditionally used to quantify endogenous proteins in these samples but require a separate assay for each protein. Recently, targeted mass spectrometry (MS) has been proposed for generating highly-multiplexed assays for biomarker proteins in DBS samples. In this work, we report the first comparison of proteins in whole blood and DBS samples using an untargeted MS approach. The average number of proteins identified in undepleted whole blood and DBS samples by liquid chromatography (LC)/MS/MS was 223 and 253, respectively. Protein identification repeatability was between 77 %–92 % within replicates and the majority of these repeated proteins (70 %) were observed in both sample formats. Proteins exclusively identified in the liquid or dried fluid spot format were unbiased based on their molecular weight, isoelectric point, aliphatic index, and grand average hydrophobicity. In addition, we extended this comparison to include proteins in matching plasma and serum samples with their dried fluid spot equivalents, dried plasma spot (DPS), and dried serum spot (DSS). This work begins to define the accessibility of endogenous proteins in dried fluid spot samples for analysis by MS and is useful in evaluating the scope of this new approach.

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Acknowledgments

The authors thank Genome Canada, Genome BC, and the Western Economic Diversification program for funding the Science and Technology Innovation Centre in Proteomics. In addition, the authors acknowledge Dr. Carol E. Parker for helpful discussions during the preparation of this manuscript.

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Authors and Affiliations

  1. Genome British Columbia Proteomics Centre, University of Victoria, Vancouver Island Technology Park, Victoria, BC, V8Z 7X8, Canada

    Andrew G. Chambers, Andrew J. Percy, Darryl B. Hardie & Christoph H. Borchers

  2. Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC, V8P 5C2, Canada

    Christoph H. Borchers

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  1. Andrew G. Chambers
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  2. Andrew J. Percy
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  4. Christoph H. Borchers
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Correspondence to Christoph H. Borchers.

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Chambers, A.G., Percy, A.J., Hardie, D.B. et al. Comparison of Proteins in Whole Blood and Dried Blood Spot Samples by LC/MS/MS. J. Am. Soc. Mass Spectrom. 24, 1338–1345 (2013). https://doi.org/10.1007/s13361-013-0678-x

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